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Effects of sertraline and sorafenib on HepG2 cells with a possible link to autophagy

  • Authors:
    • Gamze Demirel
    • Nil Pinarer
    • Mert Emre Ergin
    • Zeynep Saraçoğlu
    • Ceren Bingöl
    • Zeynep Güneş Özünal
    • Yaprak Dönmez Çakil
  • View Affiliations / Copyright

    Affiliations: Cancer and Stem Cell Center, Maltepe University, 34858 Istanbul, Türkiye, Faculty of Medicine, Maltepe University, 34858 Istanbul, Türkiye, Department of Medical Pharmacology, Faculty of Medicine, Atlas University, 34403 Istanbul, Türkiye, Department of Medical Biology and Genetics, Faculty of Medicine, Maltepe University, 34858 Istanbul, Türkiye
    Copyright: © Demirel et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Article Number: 47
    |
    Published online on: May 29, 2025
       https://doi.org/10.3892/mi.2025.246
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Abstract

Liver cancer is one of the leading causes of cancer‑related mortality worldwide. The current range of treatment options for patients with advanced‑stage disease, including the first approved systemic therapy, sorafenib, has been demonstrated to have limited efficacy. A significant number of patients develop resistance to sorafenib treatment or discontinue its use due to adverse effects. Depression is a common complication of cancer, and the antidepressant, sertraline, has recently garnered considerable attention due to its anticancer activity. Accumulating evidence suggests that autophagy may represent a highly promising target for cancer therapy. Previously, the authors demonstrated that the sorafenib and sertraline combination exerted a synergistic anticancer effect on HepG2 cells. The present study examined the intracellular localization and mRNA expression levels of key autophagy markers, including Beclin‑1, p62 and LC3, as well as the formation of acidic vesicular organelles using acridine orange staining, to further elucidate the link between autophagy and combined treatment of HepG2 cancer cells with sorafenib and sertraline. Drug treatment did not result in significant alterations in the expression levels of the LC3 and Beclin‑1 genes. However, following treatment with sertraline, sorafenib, or both, the development of acidic vesicular organelles and the noticeable formation of LC3 and p62 puncta demonstrated the induction of changes related to autophagic activity. On the whole, the results of the present study support the effects of sertraline and sorafenib, which may, at least in part, be linked to autophagy.
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Copy and paste a formatted citation
Spandidos Publications style
Demirel G, Pinarer N, Ergin ME, Saraçoğlu Z, Bingöl C, Özünal ZG and Dönmez Çakil Y: Effects of sertraline and sorafenib on HepG2 cells with a possible link to autophagy. Med Int 5: 47, 2025.
APA
Demirel, G., Pinarer, N., Ergin, M.E., Saraçoğlu, Z., Bingöl, C., Özünal, Z.G., & Dönmez Çakil, Y. (2025). Effects of sertraline and sorafenib on HepG2 cells with a possible link to autophagy. Medicine International, 5, 47. https://doi.org/10.3892/mi.2025.246
MLA
Demirel, G., Pinarer, N., Ergin, M. E., Saraçoğlu, Z., Bingöl, C., Özünal, Z. G., Dönmez Çakil, Y."Effects of sertraline and sorafenib on HepG2 cells with a possible link to autophagy". Medicine International 5.4 (2025): 47.
Chicago
Demirel, G., Pinarer, N., Ergin, M. E., Saraçoğlu, Z., Bingöl, C., Özünal, Z. G., Dönmez Çakil, Y."Effects of sertraline and sorafenib on HepG2 cells with a possible link to autophagy". Medicine International 5, no. 4 (2025): 47. https://doi.org/10.3892/mi.2025.246
Copy and paste a formatted citation
x
Spandidos Publications style
Demirel G, Pinarer N, Ergin ME, Saraçoğlu Z, Bingöl C, Özünal ZG and Dönmez Çakil Y: Effects of sertraline and sorafenib on HepG2 cells with a possible link to autophagy. Med Int 5: 47, 2025.
APA
Demirel, G., Pinarer, N., Ergin, M.E., Saraçoğlu, Z., Bingöl, C., Özünal, Z.G., & Dönmez Çakil, Y. (2025). Effects of sertraline and sorafenib on HepG2 cells with a possible link to autophagy. Medicine International, 5, 47. https://doi.org/10.3892/mi.2025.246
MLA
Demirel, G., Pinarer, N., Ergin, M. E., Saraçoğlu, Z., Bingöl, C., Özünal, Z. G., Dönmez Çakil, Y."Effects of sertraline and sorafenib on HepG2 cells with a possible link to autophagy". Medicine International 5.4 (2025): 47.
Chicago
Demirel, G., Pinarer, N., Ergin, M. E., Saraçoğlu, Z., Bingöl, C., Özünal, Z. G., Dönmez Çakil, Y."Effects of sertraline and sorafenib on HepG2 cells with a possible link to autophagy". Medicine International 5, no. 4 (2025): 47. https://doi.org/10.3892/mi.2025.246
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