Zeranol enhances leptin-induced proliferation in primary cultured human breast cancer epithelial cells

  • Authors:
    • Pingping Xu
    • Weiping Ye
    • Hong Li
    • Shu-Hong Lin
    • Chieh-Ti Kuo
    • Eric Feng
    • Young C. Lin
  • View Affiliations

  • Published online on: July 26, 2010     https://doi.org/10.3892/mmr.2010.342
  • Pages: 795-800
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Abstract

Breast cancer is the leading type of cancer in women in the United States. One of the known risk factors of breast cancer is obesity. Leptin is a product of the obese (ob) gene and plays an important role in breast cancer development. Its expression is up-regulated in obesity and it promotes breast cancer cell growth. Exposure to environmental estrogenic disruptors has been found to be directly related to the increase in the incidence of breast cancer. Zeranol (Z) is a non-steroidal anabolic growth promoter with potent estrogenic activity that is widely used in the US beef industry. The objective of this study was to determine the mechanisms of Z- and leptin-induced proliferation of primary cultured human breast cancer epithelial cells (HBCECs). A cell proliferation assay was used to determine the extent to which Z is capable of enhancing the mitogenic activity of leptin in HBCECs. RT-PCR was used to explore the possible mechanisms by quantifying the transcription of cyclin D1 and ObR genes. Our results demonstrated that when the HBCECs were pre-treated with 3 nM leptin for 24 h, the sensitivity to Z exposure greatly enhanced the mitogenic action of leptin. The experimental data observed show that there is interaction between leptin and Z in HBCEC growth.

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September-October 2010
Volume 3 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Xu P, Ye W, Li H, Lin S, Kuo C, Feng E and Lin YC: Zeranol enhances leptin-induced proliferation in primary cultured human breast cancer epithelial cells. Mol Med Rep 3: 795-800, 2010
APA
Xu, P., Ye, W., Li, H., Lin, S., Kuo, C., Feng, E., & Lin, Y.C. (2010). Zeranol enhances leptin-induced proliferation in primary cultured human breast cancer epithelial cells. Molecular Medicine Reports, 3, 795-800. https://doi.org/10.3892/mmr.2010.342
MLA
Xu, P., Ye, W., Li, H., Lin, S., Kuo, C., Feng, E., Lin, Y. C."Zeranol enhances leptin-induced proliferation in primary cultured human breast cancer epithelial cells". Molecular Medicine Reports 3.5 (2010): 795-800.
Chicago
Xu, P., Ye, W., Li, H., Lin, S., Kuo, C., Feng, E., Lin, Y. C."Zeranol enhances leptin-induced proliferation in primary cultured human breast cancer epithelial cells". Molecular Medicine Reports 3, no. 5 (2010): 795-800. https://doi.org/10.3892/mmr.2010.342