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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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November-December 2011 Volume 4 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Molecular cloning and tissue distribution of a Schistosoma japonicum gene encoding AMY-1

  • Authors:
    • Dong Hu
    • Jing Wu
    • Xiaoping Tang
    • Fengyu Hu
    • Yabo Yang
    • Jiuwei Du
    • Song Ye
    • Rongbo Zhang

  • View Affiliations / Copyright

    Affiliations: Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, Anhui 232001, P.R. China, Institute of Infectious Disease, Guangzhou No.8 People's Hospital, Guangzhou 510050, P.R. China, The Second Affiliated Hospital Of Sun Yat-sen University, Guangzhou 510120, P.R. China
  • Pages: 1267-1271
    |
    Published online on: August 22, 2011
       https://doi.org/10.3892/mmr.2011.565
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Abstract

It is well known that the mammalian associate of Myc-1 (AMY-1) plays a significant role in spermatogenesis or cellular differentiation. A full-length complementary DNA (cDNA) encoding AMY-1 of Schistosoma japonicum (SjAMY-1) was identified and isolated from a cDNA library. The gene contained an open reading frame of 315 nucleotides, encoding 105 amino acids. Sequence analysis showed that SjAMY-1 shares 65.7% homology with Homo sapiens AMY-1 amino acids and contains a conserved domain from the AMY-1 family. In this study, we cloned and expressed a recombinant SjAMY-1 (rSjAMY-1) with a molecular size of 14 kDa. The native SjAMY-1 in soluble worm antigen was identified by anti-rSjAMY-1 sera in the Western blot analysis, which demonstrated the presence of this protein in the parasite. Immunofluorescence studies indicated a localization of SjAMY-1 in various tissues and organs including the tegument and subtegumental muscles in adult worms, the ventral sucker in cercariae and the internal structures of eggs. Given the key roles of mammalian AMY-1 in cell proliferation and differentiation, the characterization of SjAMY-1 may allow for a better understanding of the development of S. japonicum.

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Copy and paste a formatted citation
Spandidos Publications style
Hu D, Wu J, Tang X, Hu F, Yang Y, Du J, Ye S and Zhang R: Molecular cloning and tissue distribution of a Schistosoma japonicum gene encoding AMY-1. Mol Med Rep 4: 1267-1271, 2011.
APA
Hu, D., Wu, J., Tang, X., Hu, F., Yang, Y., Du, J. ... Zhang, R. (2011). Molecular cloning and tissue distribution of a Schistosoma japonicum gene encoding AMY-1. Molecular Medicine Reports, 4, 1267-1271. https://doi.org/10.3892/mmr.2011.565
MLA
Hu, D., Wu, J., Tang, X., Hu, F., Yang, Y., Du, J., Ye, S., Zhang, R."Molecular cloning and tissue distribution of a Schistosoma japonicum gene encoding AMY-1". Molecular Medicine Reports 4.6 (2011): 1267-1271.
Chicago
Hu, D., Wu, J., Tang, X., Hu, F., Yang, Y., Du, J., Ye, S., Zhang, R."Molecular cloning and tissue distribution of a Schistosoma japonicum gene encoding AMY-1". Molecular Medicine Reports 4, no. 6 (2011): 1267-1271. https://doi.org/10.3892/mmr.2011.565
Copy and paste a formatted citation
x
Spandidos Publications style
Hu D, Wu J, Tang X, Hu F, Yang Y, Du J, Ye S and Zhang R: Molecular cloning and tissue distribution of a Schistosoma japonicum gene encoding AMY-1. Mol Med Rep 4: 1267-1271, 2011.
APA
Hu, D., Wu, J., Tang, X., Hu, F., Yang, Y., Du, J. ... Zhang, R. (2011). Molecular cloning and tissue distribution of a Schistosoma japonicum gene encoding AMY-1. Molecular Medicine Reports, 4, 1267-1271. https://doi.org/10.3892/mmr.2011.565
MLA
Hu, D., Wu, J., Tang, X., Hu, F., Yang, Y., Du, J., Ye, S., Zhang, R."Molecular cloning and tissue distribution of a Schistosoma japonicum gene encoding AMY-1". Molecular Medicine Reports 4.6 (2011): 1267-1271.
Chicago
Hu, D., Wu, J., Tang, X., Hu, F., Yang, Y., Du, J., Ye, S., Zhang, R."Molecular cloning and tissue distribution of a Schistosoma japonicum gene encoding AMY-1". Molecular Medicine Reports 4, no. 6 (2011): 1267-1271. https://doi.org/10.3892/mmr.2011.565
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