microrna expression signature of gastric cancer cells relative to normal gastric mucosa

Yu,Bei-Qin; Su,Li-Ping; Li,Jian-Fang; Cai,Qu; Yan,Min; Chen,Xue-Hua; Yu,Ying-Yan; Gu,Qin-Long; Zhu,Zheng-Gang; Liu,Bing-Ya

doi:10.3892/mmr.2012.1006

October 2012 Volume 6 Issue 4

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October 2012 Volume 6 Issue 4

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Article

microrna expression signature of gastric cancer cells relative to normal gastric mucosa

Authors:
- Bei-Qin Yu
- Li-Ping Su
- Jian-Fang Li
- Qu Cai
- Min Yan
- Xue-Hua Chen
- Ying-Yan Yu
- Qin-Long Gu
- Zheng-Gang Zhu
- Bing-Ya Liu
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Affiliations: Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China
Pages: 821-826
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Published online on: July 26, 2012

https://doi.org/10.3892/mmr.2012.1006
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Abstract

micrornas (miRNAs) play an important role in a wide range of physiological and developmental processes by negatively regulating the expression of target genes at the post-transcriptional level. In this study, we investigated the differential miRNA expression signature between gastric cancer cells and normal gastric mucosa to determine changes in miRNA expression during gastric cancer development. We analyzed the global miRNA expression profiles of 9 gastric cancer cell lines and 6 normal gastric mucosa lines using miRNA microarrays. In addition, we performed quantitative real-time PCR (Q-PCR) to validate the results. Correlations between the miRNA expression profile and tumor clinicopathological parameters were analyzed. We found that 17 miRNAs were upregulated in gastric cancer cell lines and 146 miRNAs were downregulated compared to normal gastric mucosa. Using microarray data and Q-PCR validation, 15 miRNAs were finally selected. These candidate miRNAs were associated with gastric cancer clinicopathology to various degrees. High expression levels of hsa-miR-93 were found to predict poor survival (median, 16 vs. 40 months; log-rank test p<0.05). These findings suggest that miRNAs play vital roles in human gastric cancer. The findings may also provide clues toward understanding the molecular functions of miRNAs in various biological processes.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

microrna expression signature of gastric cancer cells relative to normal gastric mucosa

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Abstract

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