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October 2012 Volume 6 Issue 4

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Article

Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-κB signaling in human arterial smooth muscle cells

  • Authors:
    • Hongli Li
    • Hao Xu
    • Baogui Sun
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Shanghai First People's Hospital, College of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China
  • Pages: 774-778
    |
    Published online on: July 27, 2012
       https://doi.org/10.3892/mmr.2012.1010
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Abstract

Matrix metalloproteinases (MMPs) are critical to vascular smooth muscle cell migration in vivo. The dysregulation of MMPs is involved in the pathogenesis of abnormal arterial remodeling, aneurysm formation and atherosclerotic plaque instability. It has been confirmed that lipopolysaccharides (LPS) constitute a strong risk factor for the development of atherosclerosis. In this study, we aimed to determine a potential mechanism of LPS on MMP-9 expression in human arterial smooth muscle cells (HASMCs). RT-PCR analysis was used to detect MMP-9 mRNA expression and western blot analysis was performed to examine MMP-9 protein expression. An electrophoretic mobility shift assay was also employed to determine NF-κB binding activity. Results showed that LPS induced MMP-9 mRNA and protein expression in HASMCs in a TLR4-dependent manner. Notably, upon blocking the NF-κB binding with pyrrolidine dithiocarbamate, it was demonstrated that the expression of MMP-9 by LPS occurs through TLR4/NF-κB pathways. It was concluded that LPS induced MMP-9 expression through the TLR4/NF-κB pathway. Thus, the TLR4/NF-κB pathway may be involved in the pathogenesis of atherosclerosis.
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Copy and paste a formatted citation
Spandidos Publications style
Li H, Xu H and Sun B: Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-κB signaling in human arterial smooth muscle cells. Mol Med Rep 6: 774-778, 2012.
APA
Li, H., Xu, H., & Sun, B. (2012). Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-κB signaling in human arterial smooth muscle cells. Molecular Medicine Reports, 6, 774-778. https://doi.org/10.3892/mmr.2012.1010
MLA
Li, H., Xu, H., Sun, B."Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-κB signaling in human arterial smooth muscle cells". Molecular Medicine Reports 6.4 (2012): 774-778.
Chicago
Li, H., Xu, H., Sun, B."Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-κB signaling in human arterial smooth muscle cells". Molecular Medicine Reports 6, no. 4 (2012): 774-778. https://doi.org/10.3892/mmr.2012.1010
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Xu H and Sun B: Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-κB signaling in human arterial smooth muscle cells. Mol Med Rep 6: 774-778, 2012.
APA
Li, H., Xu, H., & Sun, B. (2012). Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-κB signaling in human arterial smooth muscle cells. Molecular Medicine Reports, 6, 774-778. https://doi.org/10.3892/mmr.2012.1010
MLA
Li, H., Xu, H., Sun, B."Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-κB signaling in human arterial smooth muscle cells". Molecular Medicine Reports 6.4 (2012): 774-778.
Chicago
Li, H., Xu, H., Sun, B."Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-κB signaling in human arterial smooth muscle cells". Molecular Medicine Reports 6, no. 4 (2012): 774-778. https://doi.org/10.3892/mmr.2012.1010
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