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Article

Polydatin alleviates non-alcoholic fatty liver disease in rats by inhibiting the expression of TNF-α and SREBP-1c

  • Authors:
    • Jingming Zhang
    • Yingying Tan
    • Fanrong Yao
    • Qi Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Traditional Chinese Medicine, Basic Medical College, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712046, P.R. China, Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND 58102, USA
  • Pages: 815-820
    |
    Published online on: July 31, 2012
       https://doi.org/10.3892/mmr.2012.1015
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Abstract

The pathophysiology of non-alcoholic fatty liver disease remains incompletely elucidated, and available treatments are not entirely satisfactory. Polydatin, a stilbenoid compound derived from the rhizome of Polygonum cuspidatum, has been recognised to possess hepatoprotective and anti-inflammatory activities. The purpose of the present study was to determine whether polydatin has a protective effect against hepatic steatosis induced by a high-fat diet (HFD) and to elucidate its underlying molecular mechanisms in rats. Male Sprague-Dawley rats were randomly divided into four groups, including normal control, HFD model and polydatin‑treated groups with polydatin levels of 30 and 90 mg/kg. Following the experimental period, plasma total cholesterol (TC), triglyceride (TG) and hepatic lipid concentrations were determined. To identify a possible mechanism, we examined the changes in liver tumor necrosis factor-α (TNF-α), lipid peroxidation levels and sterol-regulatory element binding protein (SREBP-1c) mRNA and its target genes. Both 30 and 90 mg/kg polydatin treatment alleviated hepatic steatosis and reduced plasma and liver TG, TC and free fatty acid (FFA) concentration significantly in HFD rats. In addition, TNF-α, and malondialdehyde and 4-hexanonenal levels were markedly suppressed by polydatin in the liver of HFD-fed rats. Polydatin also decreased the gene expression of SREBP-1c and its target genes involved in lipogenesis, including fatty acid synthase (FAS) and stearoly-CoA desaturase 1 (SCD1) in HFD-fed rats. These results suggest that the protective effects of polydatin against HFD-induced hepatic steatosis may be partly associated with reduced liver TNF-α expression, lipid peroxidation level and SREBP-1c-mediated lipogenesis.
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1 

Duvnjak M, Lerotić I, Barsić N, Tomasić V, Virović Jukić L and Velagić V: Pathogenesis and management issues for non-alcoholic fatty liver disease. World J Gastroenterol. 13:4539–4550. 2007.PubMed/NCBI

2 

Angulo P: Non-alcoholic fatty liver disease. N Engl J Med. 346:1221–1231. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Yeh MM and Brunt EM: Pathology of non-alcoholic fatty liver disease. Am J Clin Pathol. 128:837–847. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Farrell GC and Larter CZ: Non-alcoholic fatty liver disease: from steatosis to cirrhosis. Hepatology. 43:S99–S112. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Postic C and Girard J: The role of the lipogenic pathway in the development of hepatic steatosis. Diabetes Metab. 34:643–648. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Ayyad C and Andersen T: Long-term efficacy of dietary treatment of obesity: a systematic review of studies published between 1931 and 1999. Obes Rev. 1:113–119. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Xue L: Progress in the pharmacological study of Chinese herbal drug: Polygonum cuspidatum. Zhongguo Zhong Yao Za Zhi. 25:651–653. 2000.PubMed/NCBI

8 

Luper S: A review of plants used in the treatment of liver disease: part two. Altern Med Rev. 4:178–188. 1999.PubMed/NCBI

9 

Kimura Y: Pharmacological studies on resveratrol. Methods Find Exp Clin Pharmacol. 25:297–310. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Zhang H, Dou C and Gu F: Advances in the study on pharmacological actions of Polygonum cuspidatum Sieb: clearing heat and detoxication. Zhong Yao Cai. 26:606–610. 2003.PubMed/NCBI

11 

Huang ZS, Wang ZW, Liu MP, Zhong SQ, Li QM and Rong XL: Protective effects of polydatin against CCl(4)-induced injury to primarily cultured rat hepatocytes. World J Gastroenterol. 5:41–44. 1999.PubMed/NCBI

12 

Ji H, Zhang X, Du Y, Liu H, Li S and Li L: Polydatin modulates inflammation by decreasing NF-κB activation and oxidative stress by increasing Gli1, Ptch1, SOD1 expression and ameliorates blood-brain barrier permeability for its neuroprotective effect in pMCAO rat brain. Brain Res Bull. 87:50–59. 2012.PubMed/NCBI

13 

Du J, Sun LN, Xing WW, et al: Lipid-lowering effects of polydatin from Polygonum cuspidatum in hyperlipidemic hamsters. Phytomedicine. 16:652–658. 2009. View Article : Google Scholar

14 

Xing WW, Wu JZ, Jia M, Du J, Zhang H and Qin LP: Effects of polydatin from Polygonum cuspidatum on lipid profile in hyperlipidemic rabbits. Biomed Pharmacother. 63:457–462. 2009.

15 

Zhang Q, Zhao Y, Zhang DB and Sun LJ: Effect of Sinai san decoction on the development of non-alcoholic steatohepatitis in rats. World J Gastroenterol. 11:1392–1395. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Brunt EM, Janney CG, Di Bisceglie AM, Neuschwander-Tetri BA and Bacon BR: Nonalcoholic steatohepatitis: a proposal for grading and staging the histological lesions. Am J Gastroenterol. 94:2467–2474. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Folch J, Lees M, Sloane and Starley GH: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 226:497–509. 1957.

18 

Bartlett GR: Phosphorous assay in column chromatography. J Biol Chem. 234:466–468. 1959.PubMed/NCBI

19 

Zhang Q and Tan Y: Nerve growth factor augments neuronal responsiveness to noradrenaline in cultured dorsal root ganglion neurons of rats. Neuroscience. 193:72–79. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Zhang Q, Yao F, Raizada MK, O'Rourke ST and Sun C: Apelin gene transfer into the rostral ventrolateral medulla induces chronic blood pressure elevation in normotensive rats. Circ Res. 104:1421–1428. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Diehl AM, Li ZP, Lin HZ and Yang SQ: Cytokines and the pathogenesis of non-alcoholic steatohepatitis. Gut. 54:303–306. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Tilg H and Diehl AM: Cytokines in alcoholic and nonalcoholic steatohepatitis. N Engl J Med. 343:1467–1476. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Crespo J, Cayon A, Fernandez-Gil P, et al: Gene expression of tumor necrosis factor alpha and TNF-receptors, p55 and p75, in nonalcoholic steatohepatitis patients. Hepatology. 34:1158–1163. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Abiru S, Migita K, Maeda Y, et al: Serum cytokine and soluble cytokine receptor levels in patients with non-alcoholic steatohepatitis. Liver Int. 26:39–45. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Poniachik J, Csendes A, Diaz JC, et al: Increased production of IL-1alpha and TNFalpha in lipopolysaccharide-stimulated blood from obese patients with non-alcoholic fatty liver disease. Cytokine. 33:252–257. 2006. View Article : Google Scholar

26 

Tomita K, Tamiya G, Ando S, et al: Tumour necrosis factor alpha signaling through activation of Kupffer cells plays an essential role in liver fibrosis of non-alcoholic steatohepatitis in mice. Gut. 55:415–424. 2006. View Article : Google Scholar

27 

Hui JM, Hodge A, Farrell GC, Kench JG, Kriketos A and George J: Beyond insulin resistance in NASH: TNF-alpha or adiponectin. Hepatology. 40:46–54. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Lanzilli G, Cottarelli A, Nicotera G, Guida S, Ravagnan G and Fuggetta MP: Anti-inflammatory effect of resveratrol and polydatin by in vitro IL-17 modulation. Inflammation. 35:240–248. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Wang X, Song R, Bian HN, Brunk UT, Zhao M and Zhao KS: Polydatin protects arterial smooth muscle cells against mitochondrial dysfunction and lysosomal destabilization following hemorrhagic shock. Am J Physiol Regul Integr Comp Physiol. Jan 25–2002.(Epub ahead of print).

30 

Shimano H: Sterol regulatory element-binding proteins (SREBPs): transcriptional regulators of lipid synthetic genes. Prog Lipid Res. 40:439–452. 2001. View Article : Google Scholar

31 

Raghow R, Yellaturu C, Deng X, Park EA and Elam MB: SREBPs: the crossroads of physiological and pathological lipid homeostasis. Trends Endocrinol Metab. 19:65–73. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Yan D, Lehto M, Rasilainen L, et al: Oxysterol binding protein induces upregulation of SREBP-1c and enhances hepatic lipogenesis. Arterioscler Thromb Vasc Biol. 27:1108–1114. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Yahagi N, Shimano H, Hasty AH, Matsuzaka T, Ide T and Yoshikawa T: Absence of sterol regulatory element-binding protein-1 (SREBP-1) ameliorates fatty livers but not obesity or insulin resistance in Lep(ob)/Lep(ob) mice. J Biol Chem. 277:19353–19357. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Shimomura I, Bashmakov Y and Horton JD: Increased levels of nuclear SREBP-1c associated with fatty livers in two mouse models of diabetes mellitus. J Biol Chem. 274:30028–30032. 1999. View Article : Google Scholar : PubMed/NCBI

35 

Ahmed MH and Byrne CD: Modulation of sterol regulatory element binding proteins (SREBPs) as potential treatments for non-alcoholic fatty liver disease (NAFLD). Drug Discov Today. 12:740–747. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Ntambi JM, Miyazaki M, Stoehr JP, et al: Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity. Proc Natl Acad Sci USA. 99:11482–11486. 2002. View Article : Google Scholar : PubMed/NCBI

37 

Shrestha S, Ehlers SJ, Lee JY, Fernandez ML and Koo SI: Dietary green tea extract lowers plasma and hepatic triglycerides and decreases the expression of sterol regulatory element-binding protein-1c mRNA and its responsive genes in fructose-fed, ovariectomized rats. J Nutr. 139:640–645. 2009. View Article : Google Scholar

38 

Saltiel A and Kahn CR: Insulin signalling and the regulation of glucose and lipid metabolism. Nature. 414:799–806. 2001. View Article : Google Scholar : PubMed/NCBI

39 

Browning J and Horton J: Molecular mediators of hepatic steatosis and liver injury. J Clin Invest. 114:147–152. 2004. View Article : Google Scholar : PubMed/NCBI

40 

Utzschneider KM and Kahn SE: Review: the role of insulin resistance in nonalcoholic fatty liver disease. J Clin Endocrinol Metab. 91:4753–4761. 2006. View Article : Google Scholar : PubMed/NCBI

41 

Previs SF, Withers DJ, Ren JM, White MF and Shulman GI: Contrasting effects of IRS-1 versus IRS-2 gene disruption on carbohydrate and lipid metabolism in vivo. J Biol Chem. 275:38990–38994. 2000. View Article : Google Scholar : PubMed/NCBI

42 

Samuel VT, Liu ZX, Qu X, et al: Mechanism of hepatic insulin resistance in non-alcoholic fatty liver disease. J Biol Chem. 279:32345–32353. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Tan Y, Yao F and Zhang Q: Polydatin alleviates non-alcoholic fatty liver disease in rats by inhibiting the expression of TNF-α and SREBP-1c. Mol Med Rep 6: 815-820, 2012.
APA
Zhang, J., Tan, Y., Yao, F., & Zhang, Q. (2012). Polydatin alleviates non-alcoholic fatty liver disease in rats by inhibiting the expression of TNF-α and SREBP-1c. Molecular Medicine Reports, 6, 815-820. https://doi.org/10.3892/mmr.2012.1015
MLA
Zhang, J., Tan, Y., Yao, F., Zhang, Q."Polydatin alleviates non-alcoholic fatty liver disease in rats by inhibiting the expression of TNF-α and SREBP-1c". Molecular Medicine Reports 6.4 (2012): 815-820.
Chicago
Zhang, J., Tan, Y., Yao, F., Zhang, Q."Polydatin alleviates non-alcoholic fatty liver disease in rats by inhibiting the expression of TNF-α and SREBP-1c". Molecular Medicine Reports 6, no. 4 (2012): 815-820. https://doi.org/10.3892/mmr.2012.1015
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Tan Y, Yao F and Zhang Q: Polydatin alleviates non-alcoholic fatty liver disease in rats by inhibiting the expression of TNF-α and SREBP-1c. Mol Med Rep 6: 815-820, 2012.
APA
Zhang, J., Tan, Y., Yao, F., & Zhang, Q. (2012). Polydatin alleviates non-alcoholic fatty liver disease in rats by inhibiting the expression of TNF-α and SREBP-1c. Molecular Medicine Reports, 6, 815-820. https://doi.org/10.3892/mmr.2012.1015
MLA
Zhang, J., Tan, Y., Yao, F., Zhang, Q."Polydatin alleviates non-alcoholic fatty liver disease in rats by inhibiting the expression of TNF-α and SREBP-1c". Molecular Medicine Reports 6.4 (2012): 815-820.
Chicago
Zhang, J., Tan, Y., Yao, F., Zhang, Q."Polydatin alleviates non-alcoholic fatty liver disease in rats by inhibiting the expression of TNF-α and SREBP-1c". Molecular Medicine Reports 6, no. 4 (2012): 815-820. https://doi.org/10.3892/mmr.2012.1015
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