Proteomic analysis of the cerebrospinal fluid in multiple sclerosis and neuromyelitis optica patients

Jiang,Shoufeng; Wu,Jiong; Yang,Yi; Liu,Jianren; Ding,Yao; Ding,Meiping

doi:10.3892/mmr.2012.1025

November 2012 Volume 6 Issue 5

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November 2012 Volume 6 Issue 5

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Article

Proteomic analysis of the cerebrospinal fluid in multiple sclerosis and neuromyelitis optica patients

Authors:
- Shoufeng Jiang
- Jiong Wu
- Yi Yang
- Jianren Liu
- Yao Ding
- Meiping Ding
View Affiliations / Copyright

Affiliations: Department of Neurology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Department of Neurology, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China
Pages: 1081-1086
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Published online on: August 8, 2012

https://doi.org/10.3892/mmr.2012.1025
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Abstract

The present study aimed to compare the 2-dimensional (2D) electrophoresis pattern of the cerebrospinal fluid (CSF) in multiple sclerosis (MS), neuromyelitis optica (NMO) and control individuals, to identify the proteins with differential expression and to examine their significance. CSF samples from the three groups were collected and total protein was isolated and quantified using the Bradford method. 2D electrophoresis of the samples was conducted using equal amounts of CSF. In the CSF 2D gel electrophoresis map, 118 points were obtained from the MS group, 155 points from the NMO group and 350 points from the normal control group. Non-matching proteins appeared in 14 spots in the MS group and in 45 spots in the NMO group, and were also expressed in the 2D electrophoresis pattern of the normal control group. Four differential proteins were identified through the HD-MS/MS and MASCOT network search. Pre-albumin (PA) was found only in the CSF 2D gel electrophoresis map of the MS patients. Keratin 1 was expressed in the normal control group, but not in the MS group. The difference in the expression of keratin 9 in the MS group was twice that in the normal control group. The expression of keratin 1, keratin 9 and transferrin in the NMO group was twice that in the normal control group. The expression of PA was found only in the CSF 2D gel electrophoresis map of the MS patients, and not in the NMO group. Keratin 1 was expressed in the NMO group, but not in the MS group. The expression of a variety of proteins in the 2D electrophoresis pattern of CSF was significantly different in the MS, NMO and control groups. PA, keratin 1, transferrin and keratin 9 are identified as significantly differentially expressed proteins.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Proteomic analysis of the cerebrospinal fluid in multiple sclerosis and neuromyelitis optica patients

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