Escin inhibits lipopolysaccharide-induced inflammation in human periodontal ligament cells

Liu,Shutai; Wang,Huaizhou; Qiu,Caiqing; Zhang,Jing; Zhang,Taowen; Zhou,Wenjuan; Lu,Zhishan; Rausch-Fan,Xiaohui; Liu,Zhonghao

doi:10.3892/mmr.2012.1031

November 2012 Volume 6 Issue 5

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November 2012 Volume 6 Issue 5

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Article

Escin inhibits lipopolysaccharide-induced inflammation in human periodontal ligament cells

Authors:
- Shutai Liu
- Huaizhou Wang
- Caiqing Qiu
- Jing Zhang
- Taowen Zhang
- Wenjuan Zhou
- Zhishan Lu
- Xiaohui Rausch-Fan
- Zhonghao Liu
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Affiliations: Department of Periodontology, Yantai Stomatological Hospital, Binzhou Medical University, Yantai 264008, P.R. China, Department of Anesthesiology, Yantai Stomatological Hospital, Binzhou Medical University, Yantai 264008, P.R. China, Department of Implantology, Yantai Stomatological Hospital, Binzhou Medical University, Yantai 264008, P.R. China, Department of Periodontology, Bernhard‑Gottlieb University, Clinic of Dentistry, Medical University of Vienna, Vienna A-1090, Austria
Pages: 1150-1154
|
Published online on: August 10, 2012

https://doi.org/10.3892/mmr.2012.1031
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Abstract

Periodontitis is a chronic inflammatory disease associated with gram-negative subgingival microflora infection. Accumulating experimental evidence suggests that escin exerts anti-inflammatory and anti-edematous effects. This study was designed to investigate the in vitro effects of escin on the inflammatory reaction of human periodontal ligament cells (hPDLs). hPDLs were stimulated with lipopolysaccharide (LPS). The cells were treated with various concentrations of escin. The viability of hPDLs was evaluated using the MTT method. The expression of Toll-like receptor 2 (TLR2) in hPDLs and the levels of IL-1β, TNF-α and IL-6 in the supernatant were measured. Escin significantly attenuated LPS-induced cytotoxicity in a concentration-dependent manner in hPDLs. Treatment with escin partly blocked the expression of TLR2. Escin also lowered the increase of pro-inflammatory cytokines (IL-1β, TNF-α and IL-6) induced by LPS. The present findings show that escin exerts a protective effect against LPS-induced inflammation in hPDLs. It was also shown that escin is a promising medicine for the treatment of periodontitis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Escin inhibits lipopolysaccharide-induced inflammation in human periodontal ligament cells

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