Epigallocatechin-3-gallate inhibits bladder cancer cell invasion via suppression of NF-κB‑mediated matrix metalloproteinase-9 expression

Qin,Jie; Wang,Yunbin; Bai,Yu; Yang,Kai; Mao,Qiqi; Lin,Yiwei; Kong,Debo; Zheng,Xiangyi; Xie,Liping

doi:10.3892/mmr.2012.1054

November 2012 Volume 6 Issue 5

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November 2012 Volume 6 Issue 5

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Article

Epigallocatechin-3-gallate inhibits bladder cancer cell invasion via suppression of NF-κB‑mediated matrix metalloproteinase-9 expression

Authors:
- Jie Qin
- Yunbin Wang
- Yu Bai
- Kai Yang
- Qiqi Mao
- Yiwei Lin
- Debo Kong
- Xiangyi Zheng
- Liping Xie
View Affiliations / Copyright

Affiliations: Department of Urology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China , Department of Urology, First Affiliated Hospital, Inner Mongolia Medical College, Hohhot, Inner Mongolia Autonomous Region 010050, P.R. China, Department of Urology, Tumor Hospital of Yunnan, Kunming, Yunnan 650118, P.R. China
Pages: 1040-1044
|
Published online on: August 30, 2012

https://doi.org/10.3892/mmr.2012.1054
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Abstract

Bladder cancer is the fourth most common cancer in males and the ninth most common in females. However, despite the numerous advances made in the past few decades, the prognosis of patients with bladder cancer remains poor. Metastasis is one of the major causes of mortality in bladder cancer patients. Therefore, the inhibition of metastasis is one of the most significant issues in bladder cancer research. The present study was conducted to evaluate the anti-metastatic potential of (-)-epigallocatechin gallate (EGCG, the major phytochemical in green tea) against bladder cancer and its mechanism of action. EGCG efficiently and dose-dependently inhibited adhesion, migration and invasion of T24 human bladder cancer cells. Mechanistically, EGCG inhibited phosphatidylinositol 3'-kinase/Akt activation that resulted in inactivation of nuclear factor-κB (NF-κB) and the inhibition of the expression of matrix metalloproteinase-9 (MMP-9), ultimately suppressing invasion and metastasis. These findings suggest that EGCG is a potential therapeutic candidate against tumor invasion.

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1	Ashughyan VR, Marihart S and Djavan B: Chemopreventive trials in urologic cancer. Rev Urol. 8:8–13. 2006.
2	Pectasides D, Pectasides M and Nikolaou M: Adjuvant and neoadjuvant chemotherapy in muscle invasive bladder cancer: literature review. Eur Urol. 48:60–67; discussion 67–68. 2005. View Article : Google Scholar : PubMed/NCBI
3	Khan N, Afaq F, Saleem M, Ahmad N and Mukhtar H: Targeting multiple signaling pathways by green tea polyphenol (−)-epigallo-catechin-3-gallate. Cancer Res. 66:2500–2505. 2006.
4	Yang CS, Maliakal P and Meng X: Inhibition of carcinogenesis by tea. Annu Rev Pharmacol Toxicol. 42:25–54. 2002. View Article : Google Scholar : PubMed/NCBI
5	Kemberling JK, Hampton JA, Keck RW, Gomez MA and Selman SH: Inhibition of bladder tumor growth by the green tea derivative epigallocatechin-3-gallate. J Urol. 170:773–776. 2003. View Article : Google Scholar : PubMed/NCBI
6	Liotta LA and Stetler-Stevenson WG: Tumor invasion and metastasis: an imbalance of positive and negative regulation. Cancer Res. 51(Suppl 18): 5054s–5059s. 1991.PubMed/NCBI
7	Choi YD, Cho NH, Ahn HS, Cho KS, Cho SY and Yang WJ: Matrix metalloproteinase expression in the recurrence of superficial low grade bladder transitional cell carcinoma. J Urol. 177:1174–1178. 2007. View Article : Google Scholar : PubMed/NCBI
8	Di Carlo A, Terracciano D, Mariano A and Macchia V: Urinary gelatinase activities (matrix metalloproteinases 2 and 9) in human bladder tumors. Oncol Rep. 15:1321–1326. 2006.PubMed/NCBI
9	Liu J, Zhang X, Yang F, Li T, Wei D and Ren Y: Antimetastatic effect of a lipophilic ascorbic acid derivative with antioxidation through inhibition of tumor invasion. Cancer Chemother Pharmacol. 57:584–590. 2006. View Article : Google Scholar : PubMed/NCBI
10	Suzuki Y and Isemura M: Inhibitory effect of epigallocatechin gallate on adhesion of murine melanoma cells to laminin. Cancer Lett. 173:15–20. 2001. View Article : Google Scholar : PubMed/NCBI
11	Hochman E, Castiel A, Jacob-Hirsch J, Amariglio N and Izraeli S: Molecular pathways regulating pro-migratory effects of Hedgehog signaling. J Biol Chem. 281:33860–33870. 2006. View Article : Google Scholar : PubMed/NCBI
12	Garbisa S, Biggin S, Cavallarin N, Sartor L, Benelli R and Albini A: Tumor invasion: molecular shears blunted by green tea. Nat Med. 5:12161999. View Article : Google Scholar : PubMed/NCBI
13	Meng Q, Qi M, Chen DZ, et al: Suppression of breast cancer invasion and migration by indole-3-carbinol: associated with up-regulation of BRCA1 and E-cadherin/catenin complexes. J Mol Med (Berl). 78:155–165. 2000. View Article : Google Scholar : PubMed/NCBI
14	Qin J, Xie LP, Zheng XY, et al: A component of green tea, (−)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins. Biochem Biophys Res Commun. 354:852–857. 2007.
15	Overall CM and López-Otin C: Strategies for MMP inhibition in cancer: innovations for the post-trial era. Nat Rev Cancer. 2:657–672. 2002. View Article : Google Scholar : PubMed/NCBI
16	Chakraborti S, Mandal M, Das S, Mandal A and Chakraborti T: Regulation of matrix metalloproteinases: an overview. Mol Cell Biochem. 253:269–285. 2003. View Article : Google Scholar : PubMed/NCBI
17	Kim D, Kim S, Koh H, et al: Akt/PKB promotes cancer cell invasion via increased motility and metalloproteinase production. FASEB J. 15:1953–1962. 2001. View Article : Google Scholar : PubMed/NCBI
18	Rangaswami H, Bulbule A and Kundu GC: Nuclear factor-inducing kinase plays a crucial role in osteopontin-induced MAPK/IκBα kinase-dependent nuclear factor κB-mediated promatrix metalloproteinase-9 activation. J Biol Chem. 279:38921–38935. 2004.PubMed/NCBI
19	Chiang GJ, Billmeyer BR, Canes D, et al: The src-family kinase inhibitor PP2 suppresses the in vitro invasive phenotype of bladder carcinoma cells via modulation of Akt. BJU Int. 96:416–422. 2005. View Article : Google Scholar : PubMed/NCBI
20	Rieger-Christ KM, Lee P, Zagha R, et al: Novel expression of N-cadherin elicits in vitro bladder cell invasion via the Akt signaling pathway. Oncogene. 23:4745–4753. 2004. View Article : Google Scholar : PubMed/NCBI
21	Wu X, Obata T, Khan Q, Highshaw RA, De Vere White R and Sweeney C: The phosphatidylinositol-3 kinase pathway regulates bladder cancer cell invasion. BJU Int. 93:143–150. 2004. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Epigallocatechin-3-gallate inhibits bladder cancer cell invasion via suppression of NF-κB‑mediated matrix metalloproteinase-9 expression

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