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Article

Synergistic increase in the sensitivity of osteosarcoma cells to thermochemotherapy with combination of paclitaxel and etoposide

  • Authors:
    • T. Huang
    • W. H. Gong
    • X. C. Li
    • C. P. Zou
    • G. J. Jiang
    • X. H. Li
    • D. P. Feng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
  • Pages: 1013-1017
    |
    Published online on: September 3, 2012
       https://doi.org/10.3892/mmr.2012.1058
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Abstract

Osteosarcoma is a malignant bone tumor which is found most commonly in adolescents and young adults. Local perfusion thermochemotherapy has long been proposed as an alternative strategy for the treatment of osteosarcoma. As a standard anticancer drug, paclitaxel plays a significant role in the treatment of a number of tumors; however, little is known concerning its ability to promote thermochemotherapy. The aim of this study was to evaluate the cytotoxic effects of a combination of paclitaxel and etoposide on an osteosarcoma cell line in the presence of hyperthermia and to investigate the related mechanism. Our study indicated that 1 h after the application of a combination of 10 µg/ml paclitaxel and 5 µg/ml etoposide to OS732 cells at 43˚C, the survival rate of the cells was 14.52% which was significantly lower than when either 10 µg/ml paclitaxel (45.83%) or 5 µg/ml etoposide (43.31%) was applied alone (P<0.01). Moreover, changes in cellular morphology and apoptotic rates indicated that the apoptosis-inducing effect of the combination was much stronger than that of either drug applied individually. Fas expression levels in the OS732 cells were increased by the combination of paclitaxel and etoposide in the presence of hyperthermia. Therefore, paclitaxel enhances the thermochemotherapy of the osteosarcoma cell line and this is primarily accomplished by the upregulation of Fas expression and the induction of apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Huang T, Gong WH, Li XC, Zou CP, Jiang GJ, Li XH and Feng DP: Synergistic increase in the sensitivity of osteosarcoma cells to thermochemotherapy with combination of paclitaxel and etoposide. Mol Med Rep 6: 1013-1017, 2012.
APA
Huang, T., Gong, W.H., Li, X.C., Zou, C.P., Jiang, G.J., Li, X.H., & Feng, D.P. (2012). Synergistic increase in the sensitivity of osteosarcoma cells to thermochemotherapy with combination of paclitaxel and etoposide. Molecular Medicine Reports, 6, 1013-1017. https://doi.org/10.3892/mmr.2012.1058
MLA
Huang, T., Gong, W. H., Li, X. C., Zou, C. P., Jiang, G. J., Li, X. H., Feng, D. P."Synergistic increase in the sensitivity of osteosarcoma cells to thermochemotherapy with combination of paclitaxel and etoposide". Molecular Medicine Reports 6.5 (2012): 1013-1017.
Chicago
Huang, T., Gong, W. H., Li, X. C., Zou, C. P., Jiang, G. J., Li, X. H., Feng, D. P."Synergistic increase in the sensitivity of osteosarcoma cells to thermochemotherapy with combination of paclitaxel and etoposide". Molecular Medicine Reports 6, no. 5 (2012): 1013-1017. https://doi.org/10.3892/mmr.2012.1058
Copy and paste a formatted citation
x
Spandidos Publications style
Huang T, Gong WH, Li XC, Zou CP, Jiang GJ, Li XH and Feng DP: Synergistic increase in the sensitivity of osteosarcoma cells to thermochemotherapy with combination of paclitaxel and etoposide. Mol Med Rep 6: 1013-1017, 2012.
APA
Huang, T., Gong, W.H., Li, X.C., Zou, C.P., Jiang, G.J., Li, X.H., & Feng, D.P. (2012). Synergistic increase in the sensitivity of osteosarcoma cells to thermochemotherapy with combination of paclitaxel and etoposide. Molecular Medicine Reports, 6, 1013-1017. https://doi.org/10.3892/mmr.2012.1058
MLA
Huang, T., Gong, W. H., Li, X. C., Zou, C. P., Jiang, G. J., Li, X. H., Feng, D. P."Synergistic increase in the sensitivity of osteosarcoma cells to thermochemotherapy with combination of paclitaxel and etoposide". Molecular Medicine Reports 6.5 (2012): 1013-1017.
Chicago
Huang, T., Gong, W. H., Li, X. C., Zou, C. P., Jiang, G. J., Li, X. H., Feng, D. P."Synergistic increase in the sensitivity of osteosarcoma cells to thermochemotherapy with combination of paclitaxel and etoposide". Molecular Medicine Reports 6, no. 5 (2012): 1013-1017. https://doi.org/10.3892/mmr.2012.1058
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