Regulation of glucose transporter protein-1 and vascular endothelial growth factor by hypoxia inducible factor 1α 
under hypoxic conditions in Hep-2 human cells

Xu,Ou; Li,Xiaoming; Qu,Yongtao; Liu,Shuang; An,Jie; Wang,Maoxin; Sun,Qingjia; Zhang,Wen; Lu,Xiuying; Pi,Lihong; Zhang,Min; Shen,Yupeng

doi:10.3892/mmr.2012.1075

December 2012 Volume 6 Issue 6

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December 2012 Volume 6 Issue 6

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Article

Regulation of glucose transporter protein-1 and vascular endothelial growth factor by hypoxia inducible factor 1α under hypoxic conditions in Hep-2 human cells

Authors:
- Ou Xu
- Xiaoming Li
- Yongtao Qu
- Shuang Liu
- Jie An
- Maoxin Wang
- Qingjia Sun
- Wen Zhang
- Xiuying Lu
- Lihong Pi
- Min Zhang
- Yupeng Shen
View Affiliations / Copyright

Affiliations: Department of Otorhinolaryngology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China, Department of Otorhinolaryngology Head Neck Surgery, Bethune International Peace Hospital, Shijiazhuang, Hebei 050082, P.R. China, Department of Pathology, Bethune International Peace Hospital, Shijiazhuang, Hebei 050082, P.R. China
Pages: 1418-1422
|
Published online on: September 11, 2012

https://doi.org/10.3892/mmr.2012.1075
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Abstract

The present study evaluated the regulation of glucose transporter protein-1 (Glut-1) and vascular endothelial growth factor (VEGF) by hypoxia inducible factor 1α (HIF‑1α) under hypoxic conditions in Hep-2 human cells to explore the feasibility of these three genes as tumor markers. Hep-2 cells were cultured under hypoxic and normoxic conditions for 6, 12, 24, 36 and 48 h. The proliferation of Hep-2 cells was evaluated using an MTT assay. The protein and mRNA expression levels of HIF-1α, Glut-1 and VEGF were detected using the S-P immunocytochemical method, western blotting and reverse transcription polymerase chain reaction (RT-PCR). The results revealed that the expression levels of HIF-1α, Glut-1 and VEGF protein in Hep-2 cells were significantly elevated under hypoxic conditions compared with those under normoxic conditions over 36 h. Under hypoxic conditions, mRNA levels of HIF-1α were stable, while mRNA levels of Glut-1 and VEGF changed over time. In conclusion, Glut-1 and VEGF were upregulated by HIF-1α under hypoxic conditions in a time-dependent manner in Hep-2 cells and their co-expression serves as a tumor marker.

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