Pingmu decoction enhances apoptosis of orbital adipocytes derived from patients with Graves' ophthalmophathy

Li,Hong; Wang,Yaqiong; Xu,Rongjuan

doi:10.3892/mmr.2012.1080

December 2012 Volume 6 Issue 6

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December 2012 Volume 6 Issue 6

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Article

Pingmu decoction enhances apoptosis of orbital adipocytes derived from patients with Graves' ophthalmophathy

Authors:
- Hong Li
- Yaqiong Wang
- Rongjuan Xu
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Affiliations: Department of Endocrinology, Longhua Hospital, Shanghai, P.R. China
Pages: 1361-1366
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Published online on: September 12, 2012

https://doi.org/10.3892/mmr.2012.1080
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Abstract

Graves' ophthalmopathy (GO), an autoimmune disease, has been demonstrated to result from an increased volume of orbital contents, including adipose, connective and extraocular muscle tissues. In our previous study, we showed that Pingmu decoction is capable of alleviating GO progression. In this study, to further investigate the underlying mechanism(s), we examined the effects of Pingmu decoction-containing serum on the proliferation and apoptosis of preadipocytes and adipocytes derived from the orbital adipose tissue of GO patients. Our data demonstrate for the first time that Pingmu decoction-containing serum significantly reduces preadipocyte proliferation and increases adipocyte apoptosis as measured by MTT assay and Annexin V/FITC staining, respectively. Moreover, Pingmu decoction elevated the levels of cleaved caspase-3, 8 and 9 and decreased the levels of cell cycle-related genes compared to those of the control. In addition, cell cycle arrest at the G0/G1 phase was observed following Pingmu decoction treatment. This study shows a mechanism by which Pingmu decoction serves as an effective GO medicine by downregulating preadipocyte proliferation and increasing adipocyte apoptosis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Pingmu decoction enhances apoptosis of orbital adipocytes derived from patients with Graves' ophthalmophathy

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