Immunogenicity of Cpn0425 and its localization in cells infected with Chlamydophila pneumoniae

Liu,Liangzhuan; You,Xiaoxing; Chen,Liesong; Zeng,Yanhua; Tang,Shuangyang; Yu,Minjun; Wu,Yimou; Xhen,Xi

doi:10.3892/mmr.2012.1083

December 2012 Volume 6 Issue 6

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

December 2012 Volume 6 Issue 6

Full Size Image

Article

Immunogenicity of Cpn0425 and its localization in cells infected with Chlamydophila pneumoniae

Authors:
- Liangzhuan Liu
- Xiaoxing You
- Liesong Chen
- Yanhua Zeng
- Shuangyang Tang
- Minjun Yu
- Yimou Wu
- Xi Xhen
View Affiliations / Copyright

Affiliations: Pathogenic Biology Institute, University of South China, Hengyang 421001, P.R. China, Hunan Provincial Center of Disease Prevention and Control, Changsha 410005, P.R. China
Pages: 1239-1242
|
Published online on: September 13, 2012

https://doi.org/10.3892/mmr.2012.1083
Expand metrics +

Abstract

The present study aimed to determine the intracellular localization of Cpn0425 in Chlamydophila pneumoniae-infected cells. The recombinant plasmid pGEX-6p/Cpn0425 was transformed into E.coli Bl21 cells to express the fusion protein. Following purification with glutathione S-transferase (GST) resin chromatography, the Cpn0425 fusion protein was used to induce immunity in mice to develop monoclonal and polyclonal antibodies, which were subsequently used to localize the endogenous Cpn0425 protein by indirect immunofluorescence assay (IFA). ELISA was used to determine the immunogenicity of the Cpn0425 plasmid protein by recognizing the pool sera of patients infected with Chlamydia trachomatis and the pool sera of mice immunized with the Cpn0425 fusion protein. The Cpn0425 gene was expressed as the GST-Cpn0425 fusion protein in E. coli and its antibody was prepared by immunizing mice with the fusion protein. An anti-GST-Cpn0425 antibody was used to localize the protein in cells infected with Chlamydophila pneumoniae AR-39 using an IFA. The anti-GST-CT058 antibody detected an inclusion signal in the IFA. Cpn0425 protein strongly reacted with antiserum. Although Cpn0425 protein is not a secreted protein, it has good immunogenicity. Therefore, this protein may be useful for developing vaccines against Chlamydophila pneumoniae infection.

View Figures

View References

1	Lui G, Ip M, Lee N, et al: Role of ‘atypical pathogens’ among adult hospitalized patients with community-acquired pneumonia. Respirology. 14:1098–1105. 2009.
2	Damy SB, Higuchi ML, Timenetsky J, et al: Mycoplasma pneumoniae and/or Chlamydophila pneumoniae inoculation causing different aggravations in cholesterol-induced atherosclerosis in apoE KO male mice. BMC Microbiol. 9:1942009. View Article : Google Scholar
3	Papaetis GS, Anastasakou E and Orphanidou D: Chlamydophila pneumoniae infection and COPD: more evidence for lack of evidence? Eur J Intern Med. 20:579–785. 2009. View Article : Google Scholar
4	Song JH, Thamlikitkul V and Hsueh PR: Clinical and economic burden of community-acquired pneumonia amongst adults in the Asia-Pacific region. Int J Antimicrob Agents. 38:108–117. 2011.PubMed/NCBI
5	Jackson RW, Vinatzer B, Arnold DL, Dorus S and Murillo J: The influence of the accessory genome on bacterial pathogen evolution. Mob Genet Elements. 1:55–65. 2011. View Article : Google Scholar : PubMed/NCBI
6	Mota LJ: Cornelis. The bacterial injection kit: type III secretion systems. Ann Med. 37:234–249. 2005. View Article : Google Scholar : PubMed/NCBI
7	Koizumi Y, Toma C, Higa N, Nohara T, Nakasone N and Suzuki T: Inflammasome activation via intracellular NLRs triggered by bacterial infection. Cell Microbiol. 14:149–154. 2012. View Article : Google Scholar : PubMed/NCBI
8	Diaz MR, King JM and Yahr TL: Intrinsic and extrinsic regulation of type III secretion gene expression in pseudomonas aeruginosa. Front Microbiol. 2:892011.PubMed/NCBI
9	Dean P: Functional domains and motifs of bacterial type III effector proteins and their roles in infection. FEMS Microbiol Rev. 35:1100–1125. 2011. View Article : Google Scholar : PubMed/NCBI
10	Park SH, Kwon SJ, Lee SJ, Kim YC, Hwang KY, Kang YH and Lee KJ: Identification of immunogenic antigen candidate for Chlamydophila pneumoniae diagnosis. J Proteome Res. 8:2933–2943. 2009. View Article : Google Scholar : PubMed/NCBI
11	Tammiruusu A, Penttilä T, Lahesmaa R, Sarvas M, Puolakkainen M and Vuola JM: Intranasal administration of chlamydial outer protein N (CopN) induces protection against pulmonary Chlamydia pneumoniae infection in a mouse model. Vaccine. 25:283–290. 2007. View Article : Google Scholar : PubMed/NCBI
12	Kalman S, Mitchell W, Marathe R, et al: Comparative genomes of Chlamydia pneumoniae and C. trachomatis. Nat Genet. 21:385–389. 1999. View Article : Google Scholar
13	Chen CQ, Wu YM, Li ZY, et al: Chlamydia trachomatis outer membrane protein expression of recombinant protein purification and immunological identification. Chin Lab Med. 28:697–699. 2005.
14	Lugert R, Kuhns M, Polch T and Gross U: Expression and localization of type III secretion-related proteins of Chlamydia pneumoniae. Med Microbiol Immunol. 193:163–171. 2004. View Article : Google Scholar : PubMed/NCBI
15	Timms P, Good D, Wan C, Theodoropoulos C, Mukhopadhyay S, Summersgill J and Mathews S: Differential transcriptional responses between the interferon-gamma-induction and iron-limitation models of persistence for Chlamydia pneumoniae. J Microbiol Immunol Infect. 42:27–37. 2009.PubMed/NCBI
16	Chen C, Chen D, Sharma J, et al: The hypothetical protein CT813 is localized in the Chlamydia trachomatis inclusion membrane and is immunogenic in women urogenitally infected with C. trachomatis. Infect Immun. 74:4826–4840. 2006.PubMed/NCBI
17	Huang Z, Feng Y, Chen D, et al: Structural basis for activation and inhibition of the secreted chlamydia protease CPAF. Cell Host Microbe. 4:529–542. 2008. View Article : Google Scholar : PubMed/NCBI
18	Pirbhai M, Dong F, Zhong Y, et al: The secreted protease factor CPAF is responsible for degrading pro-apoptotic BH3-only proteins in Chlamydia trachomatis-infected cells. J Biol Chem. 281:31495–31501. 2006. View Article : Google Scholar : PubMed/NCBI
19	Li ZY, Wang SP, Wu YM, et al: Immunization with chlamydial plasmid protein pORF5 DNA vaccine induces protective immunity against genital chlamydial infection in mice. Sci China C Life Sci. 51:973–980. 2008. View Article : Google Scholar : PubMed/NCBI
20	Toh H, Miura K, Shirai M, et al: In silico inference of inclusion membrane protein family in obligate intracellular parasites chlamydiae. DNA Res. 10:9–17. 2003. View Article : Google Scholar

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Immunogenicity of Cpn0425 and its localization in cells infected with Chlamydophila pneumoniae

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Related Articles