CD151 promotes cancer cell metastasis via integrins α3β1 and α6β1 in vitro

Fei,Yujie; Wang,Ji; Liu,Weifeng; Zuo,Houjuan; Qin,Jin; Wang,Daowen; Zeng,Hesong; Liu,Zhengxiang

doi:10.3892/mmr.2012.1095

December 2012 Volume 6 Issue 6

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December 2012 Volume 6 Issue 6

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Article

CD151 promotes cancer cell metastasis via integrins α3β1 and α6β1 in vitro

Authors:
- Yujie Fei
- Ji Wang
- Weifeng Liu
- Houjuan Zuo
- Jin Qin
- Daowen Wang
- Hesong Zeng
- Zhengxiang Liu
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Affiliations: Department of Cardiology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, P.R. China, Department of General Surgery, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, P.R. China, Department of Cardiology of Central Hospital of Zibo, Zibo 255000, P.R. China
Pages: 1226-1230
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Published online on: September 24, 2012

https://doi.org/10.3892/mmr.2012.1095
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Abstract

CD151 is a member of the tetraspanin family that is implicated as a promoter of the tumor metastasis of malignant cells. Tetraspanins form membrane complexes with integrins. In the present study, we constructed a CD151-AAA mutant to assess the roles of Rac, cdc42 and phospho-Rac/cdc42 (P-Rac/cdc42) and the effects of CD151‑integrin complexes on the proliferation, migration and invasion of HepG2 cells. The pAAV-CD151 and pAAV‑CD151‑AAA mutant plasmids were constructed and used to transiently transfect HepG2 cells using the Qiagen Attractene transfection reagent. Following transfection, the expression of CD151 was determined by western blotting. A cell proliferation assay was performed using the cell counting kit-8 (CCK-8) method, cell migration was assessed by a cell wound-healing assay and cell invasion was evaluated in microchemotaxis chambers using FBS as the chemotactic stimulus. The potential involvement of various signaling pathways was explored using relevant antibodies. The association between CD151 and integrins was evaluated by immunoblotting analysis. We found that CD151 promoted cell proliferation, migration and chemotaxis and increased P-Rac/cdc42 activity. The CD151-AAA mutant had reduced cellular proliferation, migration and invasion compared with the CD151 mutant. Moreover, the CD151-AAA mutant abrogated the association between CD151 and integrins. These data suggest that CD151 forms complexes by interacting with integrins, particularly α3β1 and α6β1, and thereby affects the functioning of the HepG2 cells. The mechanism is possibly related to the Rac, cdc42 and P-Rac/cdc42 signaling pathways.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

CD151 promotes cancer cell metastasis via integrins α3β1 and α6β1 in vitro

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