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Article

IGF-1 activates the P13K/AKT signaling pathway via upregulation of secretory clusterin

  • Authors:
    • Xiumei Ma
    • Yongrui Bai
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200120, P.R. China
  • Pages: 1433-1437
    |
    Published online on: September 28, 2012
       https://doi.org/10.3892/mmr.2012.1110
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Abstract

Secretory clusterin (sCLU) is a type of stress-induced, pro-survival glycoprotein elevated in early-stage cancer. It enhances cancer cell survival and is associated with several types of cancer progression. In this study, we measured the PI3K/AKT signaling activity by determining the phosphorylation level of the AKT protein, namely pAKT. A549 human non-small cell lung carcinoma (NSCLC) cells were treated with insulin-like growth factor-1 (IGF-1) for various periods of time. The results showed that IGF-1 activated the PI3K/AKT signaling pathway in the A549 cells in a time-dependent manner. Western blot analysis was performed to determine the expression of sCLU protein in A549 cells treated with IGF-1. IGF-1 elevated the expression of sCLU. To determine whether sCLU is required for the IGF-1 activation of the PI3K/AKT signaling pathway, the A549 cells were treated with IGF-1 and sCLU antisense oligonuleotide (sCLU ASO). sCLU ASO blocked the IGF-1 activation of the PI3K/AKT signaling pathway. These results demonstrate that IGF-1 activates the P13K/AKT signaling pathway via the upregulation of sCLU. The present study implies that this pathway may uncover a new mechanism for cancer progression and reveal new targets for drug development in the treatment of NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Ma X and Bai Y: IGF-1 activates the P13K/AKT signaling pathway via upregulation of secretory clusterin. Mol Med Rep 6: 1433-1437, 2012.
APA
Ma, X., & Bai, Y. (2012). IGF-1 activates the P13K/AKT signaling pathway via upregulation of secretory clusterin. Molecular Medicine Reports, 6, 1433-1437. https://doi.org/10.3892/mmr.2012.1110
MLA
Ma, X., Bai, Y."IGF-1 activates the P13K/AKT signaling pathway via upregulation of secretory clusterin". Molecular Medicine Reports 6.6 (2012): 1433-1437.
Chicago
Ma, X., Bai, Y."IGF-1 activates the P13K/AKT signaling pathway via upregulation of secretory clusterin". Molecular Medicine Reports 6, no. 6 (2012): 1433-1437. https://doi.org/10.3892/mmr.2012.1110
Copy and paste a formatted citation
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Spandidos Publications style
Ma X and Bai Y: IGF-1 activates the P13K/AKT signaling pathway via upregulation of secretory clusterin. Mol Med Rep 6: 1433-1437, 2012.
APA
Ma, X., & Bai, Y. (2012). IGF-1 activates the P13K/AKT signaling pathway via upregulation of secretory clusterin. Molecular Medicine Reports, 6, 1433-1437. https://doi.org/10.3892/mmr.2012.1110
MLA
Ma, X., Bai, Y."IGF-1 activates the P13K/AKT signaling pathway via upregulation of secretory clusterin". Molecular Medicine Reports 6.6 (2012): 1433-1437.
Chicago
Ma, X., Bai, Y."IGF-1 activates the P13K/AKT signaling pathway via upregulation of secretory clusterin". Molecular Medicine Reports 6, no. 6 (2012): 1433-1437. https://doi.org/10.3892/mmr.2012.1110
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