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December 2012 Volume 6 Issue 6

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Article

Cytokine expression and the role of Th17 cells in a mouse model of colitis

  • Authors:
    • Ying He
    • Lian-Jie Lin
    • Chang-Qing Zheng
    • Yu Jin
    • Yan Lin
  • View Affiliations / Copyright

    Affiliations: Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
  • Pages: 1438-1442
    |
    Published online on: September 28, 2012
       https://doi.org/10.3892/mmr.2012.1111
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Abstract

The aim of this study was to explore the expression of cytokines by Th17 cells and their mechanisms of action in a mouse model of 2,4,6-trinitrobenzenesulfonic acid (TNBS)‑induced inflammatory bowel disease (IBD). ELISA was used to detect the expression of the Th17 cytokine interleukin, (IL)-17, and that of the Th1 cytokine, interferon-γ (IFN-γ), in colon tissues. Western blot analysis was used to detect IL-17 expression in the peripheral blood mononuclear cells (PBMCs), spleen mononuclear cells (SMCs), mesenteric lymph node cells and colon tissues of the colitic mice. RT-PCR analysis was used to detect the effect of anti-IL-17 antibody application on the tumor necrosis factor (TNF)-α, IFN-γ and IL-6 mRNA levels in the SMCs of the colitic mice. The Th17 cytokine, IL-17, and the Th1 cytokine, IFN-γ, were expressed at high levels in the TNBS‑induced colitic mice. In addition, the expression of the Th17 cytokine appeared earlier than that of the Th1 cytokine. The IL-17 levels in the SMCs, mesenteric lymph node cells and colon tissues of the disease model group were significantly different from those of the normal control group (p<0.01), while the IL-17 levels in the PBMCs of the disease model group were not significantly different (p>0.05) from those of the control group. Following the application of 10 µg/ml anti‑IL‑7 antibody, the TNF-α, IL-6 and IFN-γ mRNA levels in the SMCs of the model group demonstrated no significant differences from those of the non-antibody‑treated control group (p>0.05). In conclusion, Th17 and Th1 cells are involved in TNBS-induced IBD and the effect of the Th17 cells may be mediated through the induction of the secretion of pro-inflammatory cytokines.
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Copy and paste a formatted citation
Spandidos Publications style
He Y, Lin L, Zheng C, Jin Y and Lin Y: Cytokine expression and the role of Th17 cells in a mouse model of colitis. Mol Med Rep 6: 1438-1442, 2012.
APA
He, Y., Lin, L., Zheng, C., Jin, Y., & Lin, Y. (2012). Cytokine expression and the role of Th17 cells in a mouse model of colitis. Molecular Medicine Reports, 6, 1438-1442. https://doi.org/10.3892/mmr.2012.1111
MLA
He, Y., Lin, L., Zheng, C., Jin, Y., Lin, Y."Cytokine expression and the role of Th17 cells in a mouse model of colitis". Molecular Medicine Reports 6.6 (2012): 1438-1442.
Chicago
He, Y., Lin, L., Zheng, C., Jin, Y., Lin, Y."Cytokine expression and the role of Th17 cells in a mouse model of colitis". Molecular Medicine Reports 6, no. 6 (2012): 1438-1442. https://doi.org/10.3892/mmr.2012.1111
Copy and paste a formatted citation
x
Spandidos Publications style
He Y, Lin L, Zheng C, Jin Y and Lin Y: Cytokine expression and the role of Th17 cells in a mouse model of colitis. Mol Med Rep 6: 1438-1442, 2012.
APA
He, Y., Lin, L., Zheng, C., Jin, Y., & Lin, Y. (2012). Cytokine expression and the role of Th17 cells in a mouse model of colitis. Molecular Medicine Reports, 6, 1438-1442. https://doi.org/10.3892/mmr.2012.1111
MLA
He, Y., Lin, L., Zheng, C., Jin, Y., Lin, Y."Cytokine expression and the role of Th17 cells in a mouse model of colitis". Molecular Medicine Reports 6.6 (2012): 1438-1442.
Chicago
He, Y., Lin, L., Zheng, C., Jin, Y., Lin, Y."Cytokine expression and the role of Th17 cells in a mouse model of colitis". Molecular Medicine Reports 6, no. 6 (2012): 1438-1442. https://doi.org/10.3892/mmr.2012.1111
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