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Article

In vivo and in vitro evidence of the neurotoxic effects of ropivacaine: The role of the Akt signaling pathway

  • Authors:
    • Zhihua Sun
    • Huining Liu
    • Qulian Guo
    • Xiaoping Xu
    • Zhong Zhang
    • Na Wang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, P.R. China, Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, P.R. China, Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, P.R. China
  • Pages: 1455-1459
    |
    Published online on: October 1, 2012
       https://doi.org/10.3892/mmr.2012.1115
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Abstract

There is a growing body of evidence that suggests common complications in regional anesthesia, including transient neurological syndrome, are caused by the neurotoxicity of local anesthetics (LAs). Ropivacaine is thought to be one of the safest LAs, however, there have been several studies detailing possible neurotoxic effects. At present, the exact molecular mechanism of ropivacaine-mediated neurotoxicity is unknown. The present study was designed to explore the possible mechanisms underlying the neurotoxicity of ropivacaine. The neurotoxic effects of ropivacaine were assessed in spinal cord by TUNEL staining for apoptosis and in cultured PC12 cells by cell viability assays. Protein kinase B (Akt) activation was evaluated by immunoblotting. Ropivacaine promoted apoptosis and caused cell death in a treatment group compared with a sham-operated group. Furthermore, ropivacaine significantly diminished Akt activation. There were significantly lower Akt levels in cells exposed to ropivacaine compared with controls. The present study demonstrated ropivacaine neurotoxicity in vivo and in vitro, mediated by the Akt signaling pathway. The neurotoxicity of apoptosis with concomitant cell death, mediated by ropivacaine, may offer an explanation for its adverse effects (e.g., transient neurological syndrome).
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Copy and paste a formatted citation
Spandidos Publications style
Sun Z, Liu H, Guo Q, Xu X, Zhang Z and Wang N: In vivo and in vitro evidence of the neurotoxic effects of ropivacaine: The role of the Akt signaling pathway. Mol Med Rep 6: 1455-1459, 2012.
APA
Sun, Z., Liu, H., Guo, Q., Xu, X., Zhang, Z., & Wang, N. (2012). In vivo and in vitro evidence of the neurotoxic effects of ropivacaine: The role of the Akt signaling pathway. Molecular Medicine Reports, 6, 1455-1459. https://doi.org/10.3892/mmr.2012.1115
MLA
Sun, Z., Liu, H., Guo, Q., Xu, X., Zhang, Z., Wang, N."In vivo and in vitro evidence of the neurotoxic effects of ropivacaine: The role of the Akt signaling pathway". Molecular Medicine Reports 6.6 (2012): 1455-1459.
Chicago
Sun, Z., Liu, H., Guo, Q., Xu, X., Zhang, Z., Wang, N."In vivo and in vitro evidence of the neurotoxic effects of ropivacaine: The role of the Akt signaling pathway". Molecular Medicine Reports 6, no. 6 (2012): 1455-1459. https://doi.org/10.3892/mmr.2012.1115
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Z, Liu H, Guo Q, Xu X, Zhang Z and Wang N: In vivo and in vitro evidence of the neurotoxic effects of ropivacaine: The role of the Akt signaling pathway. Mol Med Rep 6: 1455-1459, 2012.
APA
Sun, Z., Liu, H., Guo, Q., Xu, X., Zhang, Z., & Wang, N. (2012). In vivo and in vitro evidence of the neurotoxic effects of ropivacaine: The role of the Akt signaling pathway. Molecular Medicine Reports, 6, 1455-1459. https://doi.org/10.3892/mmr.2012.1115
MLA
Sun, Z., Liu, H., Guo, Q., Xu, X., Zhang, Z., Wang, N."In vivo and in vitro evidence of the neurotoxic effects of ropivacaine: The role of the Akt signaling pathway". Molecular Medicine Reports 6.6 (2012): 1455-1459.
Chicago
Sun, Z., Liu, H., Guo, Q., Xu, X., Zhang, Z., Wang, N."In vivo and in vitro evidence of the neurotoxic effects of ropivacaine: The role of the Akt signaling pathway". Molecular Medicine Reports 6, no. 6 (2012): 1455-1459. https://doi.org/10.3892/mmr.2012.1115
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