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Article

Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line

  • Authors:
    • Shuangyi Ren
    • Hailong Chen
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    Affiliations: Department of Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China, Department of Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
  • Pages: 166-170
    |
    Published online on: October 12, 2012
       https://doi.org/10.3892/mmr.2012.1124
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Abstract

Apoptosis-associated speck-like protein (ASC) is an adaptor molecule of caspase-1 activation that stimulates the secretion of multiple pro-inflammatory cytokines. We investigated the regulatory effects of ASC in the P388D1 macrophage-like cell line. Data showed that ASC overexpression induced by pEGFP-ASC-C2 transfection significantly increased caspase-1 expression and interleukin (IL)-1β and IL-6 secretion, but did not affect tumor necrosis factor (TNF)-α secretion. However, siRNA against ASC significantly decreased the caspase-1 expression and IL-1β and IL-6 secretion, but did not affect TNF-α secretion in the P388D1 cells. We suggest that ASC acts as an inflammatory response-associated gene by regulating caspase-1 activation and IL-1β and IL-6 secretion, which may correlate with its biological effects.
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Copy and paste a formatted citation
Spandidos Publications style
Ren S and Chen H: Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line. Mol Med Rep 7: 166-170, 2013.
APA
Ren, S., & Chen, H. (2013). Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line. Molecular Medicine Reports, 7, 166-170. https://doi.org/10.3892/mmr.2012.1124
MLA
Ren, S., Chen, H."Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line". Molecular Medicine Reports 7.1 (2013): 166-170.
Chicago
Ren, S., Chen, H."Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line". Molecular Medicine Reports 7, no. 1 (2013): 166-170. https://doi.org/10.3892/mmr.2012.1124
Copy and paste a formatted citation
x
Spandidos Publications style
Ren S and Chen H: Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line. Mol Med Rep 7: 166-170, 2013.
APA
Ren, S., & Chen, H. (2013). Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line. Molecular Medicine Reports, 7, 166-170. https://doi.org/10.3892/mmr.2012.1124
MLA
Ren, S., Chen, H."Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line". Molecular Medicine Reports 7.1 (2013): 166-170.
Chicago
Ren, S., Chen, H."Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line". Molecular Medicine Reports 7, no. 1 (2013): 166-170. https://doi.org/10.3892/mmr.2012.1124
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