Effect of microtopographic structures of silk fibroin on endothelial cell behavior

Tan,Jin-Yun; Wen,Jian-Chuan; Shi,Wei-Hao; He,Qing; Zhu,Lei; Liang,Kun; Shao,Zheng-Zhong; Yu,Bo

doi:10.3892/mmr.2012.1127

Effect of microtopographic structures of silk fibroin on endothelial cell behavior

Authors:
- Jin-Yun Tan
- Jian-Chuan Wen
- Wei-Hao Shi
- Qing He
- Lei Zhu
- Kun Liang
- Zheng-Zhong Shao
- Bo Yu
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Affiliations: Department of Surgery, Huashan Hospital of Fudan University, Shanghai 200040, P.R. China, Laboratory of Advanced Materials of Fudan University, Shanghai 200438, P.R. China, Department of Surgery, Pudong Hospital of Fudan University, Shanghai 201399, P.R. China
Published online on: October 12, 2012 https://doi.org/10.3892/mmr.2012.1127
Pages: 292-298

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Abstract

Stent implantation has become the preferred revascularization treatment for occlusive blood vessel disease; however, there are occasionally complications resulting in re-narrowing of the treated artery. One approach to overcoming this problem is to establish a confluent monolayer of endothelial cells (ECs) on the stent, and a coating would facilitate the attachment of ECs. Silk fibroin was reported to be used as an ideal coating applied to stent for the culture of human ECs. The aim of the present study is to gain more insight into the influence of the internal microtopographical structure of silk fibroin on cell behavior, such as attachment and growth, and to further investigate its molecular mechanism using human umbilical vein ECs (HUVECs). Our results evaluated the effect of different microtopographical structures on cell behavior. In addition, we analyzed the cell cycle and investigated relevant molecules involved. The results indicated that the microtopographic structure of silk fibroin was associated with EC morphology, attachment and proliferation.

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1	Hinohara T: Percutaneous coronary intervention: current perspective. Keio J Med. 50:152–160. 2001. View Article : Google Scholar : PubMed/NCBI
2	Alfonso F, Pérez-Vizcayno MJ, Cruz A, García J, Jimenez-Quevedo P, Escaned J and Hernandez R: Treatment of patients with in-stent restenosis. EuroIntervention. 5(Suppl D): D70–D78. 2009.PubMed/NCBI
3	Thansayari P, Kathir K, Celemajer DS and Adams MR: Endothelial dysfunction and restenosis following percutaneous coronary intervention. Int J Cardiol. 119:362–367. 2007. View Article : Google Scholar : PubMed/NCBI
4	Bonetti PO, Lerman LO and Lerman A: Endothelial dysfunction: a marker of atherosclerotic risk. Arterioscler Thromb Vasc Biol. 23:168–175. 2003. View Article : Google Scholar : PubMed/NCBI
5	Kipshidze N, Baker J and Nikolaychik N: Fibrin coated stents as an improved vehicle for endothelial cell seeding. Circulation. 90:I-5971994.
6	Flugelman MY, Virmani R, Leon MB, Bowman RL and Dichek DA: Genetically engineered endothelial cells remain adherent and viable after stent deployment and exposure to flow in vitro. Circ Res. 70:348–354. 1992. View Article : Google Scholar
7	Scott NA, Candal FJ, Robinson KA and Ades EW: Seeding of intracoronary stents with immortalized human microvascular endothelial cells. Am Heart J. 129:860–866. 1995. View Article : Google Scholar : PubMed/NCBI
8	Van der Giessen WJ, Serruys PW, Visser WJ, Verdouw PD, van Schalkwijk WP and Jongkind JF: Endothelialization of intravascular stents. J Intervent Cardiol. 1:109–120. 1988.
9	Dichek DA, Neville RF, Zwiebel JA, Freeman SM, Leon MB and Anderson WF: Seeding of intravascular stents with genetically engineered endothelial cells. Circulation. 80:1347–1353. 1989. View Article : Google Scholar : PubMed/NCBI
10	Chen MC, Liang HF, Chiu YL, Chang Y, Wei HJ and Sung HW: A novel drug-eluting stent spray-coated with multi-layers of collagen and sirolimus. J Control Release. 108:178–189. 2005. View Article : Google Scholar : PubMed/NCBI
11	Thierry B, Winnik FM, Merhi Y, Silver J and Tabrizian M: Bioactive coatings of endovascular stents based on polyelectrolyte multilayers. Biomacromolecules. 4:1564–1571. 2003. View Article : Google Scholar : PubMed/NCBI
12	Zhang X, Baughman CB and Kaplan DL: In vitro evaluation of electrospun silk fibroin scaffolds for vascular cell growth. Biomaterials. 29:2217–2227. 2008. View Article : Google Scholar : PubMed/NCBI
13	Wang X, Zhang X, Castellot J, Herman I, Iafrati M and Kaplan DL: Controlled release from multilayer silk biomaterial coatings to modulate vascular cell responses. Biomaterials. 29:894–903. 2008. View Article : Google Scholar : PubMed/NCBI
14	Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J and Kaplan DL: Silk-based biomaterials. Biomaterials. 24:401–416. 2003. View Article : Google Scholar : PubMed/NCBI
15	Diener A, Nebe B, Lüthen F, Becker P, Beck U, Neumann HG and Rychly J: Control of focal adhesion dynamics by material surface characteristics. Biomaterials. 26:383–392. 2005. View Article : Google Scholar : PubMed/NCBI
16	Karuri NW, Porri TJ, Albrecht RM, Murphy CJ and Nealey PF: Nano- and microscale holes modulate cell-substrate adhesion, cytoskeletal organization, and -beta1 integrin localization in SV40 human corneal epithelial cells. IEEE Trans Nanobioscience. 5:273–280. 2006. View Article : Google Scholar
17	Jaffe EA, Nachman RL, Becker CG and Minick CR: Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 52:2745–2756. 1973. View Article : Google Scholar : PubMed/NCBI
18	Teixeira AI, Nealey PF and Murphy CJ: Responses of human keratocytes to micro- and nano structured substrates. J Biomed Mater Res A. 71:369–376. 2004. View Article : Google Scholar : PubMed/NCBI
19	Rebollar E, Frischauf I, Olbrich M, Peterbauer T, Hering S, Preiner J, Hinterdorfer P, Romanin C and Heitz J: Proliferation of aligned mammalian cells on laser-nanostructured polystyrene. Biomaterials. 29:1796–1806. 2008. View Article : Google Scholar : PubMed/NCBI
20	Roca-Cusachs P, Alcaraz J, Sunyer R, Samitier J, Farré R and Navajas D: Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation. Biophys J. 94:4984–4995. 2008.PubMed/NCBI