Effect of microtopographic structures of silk fibroin on endothelial cell behavior

Tan,Jin-Yun; Wen,Jian-Chuan; Shi,Wei-Hao; He,Qing; Zhu,Lei; Liang,Kun; Shao,Zheng-Zhong; Yu,Bo

doi:10.3892/mmr.2012.1127

January 2013 Volume 7 Issue 1

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January 2013 Volume 7 Issue 1

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Article

Effect of microtopographic structures of silk fibroin on endothelial cell behavior

Authors:
- Jin-Yun Tan
- Jian-Chuan Wen
- Wei-Hao Shi
- Qing He
- Lei Zhu
- Kun Liang
- Zheng-Zhong Shao
- Bo Yu
View Affiliations / Copyright

Affiliations: Department of Surgery, Huashan Hospital of Fudan University, Shanghai 200040, P.R. China, Laboratory of Advanced Materials of Fudan University, Shanghai 200438, P.R. China, Department of Surgery, Pudong Hospital of Fudan University, Shanghai 201399, P.R. China
Pages: 292-298
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Published online on: October 12, 2012

https://doi.org/10.3892/mmr.2012.1127
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Abstract

Stent implantation has become the preferred revascularization treatment for occlusive blood vessel disease; however, there are occasionally complications resulting in re-narrowing of the treated artery. One approach to overcoming this problem is to establish a confluent monolayer of endothelial cells (ECs) on the stent, and a coating would facilitate the attachment of ECs. Silk fibroin was reported to be used as an ideal coating applied to stent for the culture of human ECs. The aim of the present study is to gain more insight into the influence of the internal microtopographical structure of silk fibroin on cell behavior, such as attachment and growth, and to further investigate its molecular mechanism using human umbilical vein ECs (HUVECs). Our results evaluated the effect of different microtopographical structures on cell behavior. In addition, we analyzed the cell cycle and investigated relevant molecules involved. The results indicated that the microtopographic structure of silk fibroin was associated with EC morphology, attachment and proliferation.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of microtopographic structures of silk fibroin on endothelial cell behavior

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