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Article

Anticancer activities of tanshinone microemulsion against hepatocellular carcinoma in vitro and in vivo

  • Authors:
    • Hui Ma
    • Qing Fan
    • Jia Yu
    • Jile Xin
    • Ce Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116027, P.R. China
  • Pages: 59-64
    |
    Published online on: October 12, 2012
       https://doi.org/10.3892/mmr.2012.1129
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Abstract

The natural product tanshinone (Tan) induces apoptosis and differentiation in hepatocellular carcinoma (HCC) cells, but its clinical use is limited by its poor water solubility and the lack of appropriate formulations. In this study, Tan was encapsulated into a microemulsion (ME) composed of phospholipid, ethyl oleate, glycerol and Pluronic F68. The anticancer effects and mechanisms of action of Tan ME were tested using in vitro and in vivo HCC models. The mRNA and protein levels of apoptosis related molecules (Bcl-2 and Bax) were analyzed in H22 murine hepatoma cells and H22 tumor-bearing mice by flow cytometry, RT-PCR and immunofluorescence staining. Compared with empty ME and drug solution groups, the mRNA levels of Bax were upregulated and the mRNA and protein levels of Bcl-2 were downregulated in the H22 cells treated with Tan ME in a dose-dependent manner. The mRNA and protein levels of Bax were upregulated and the Bcl-2 levels were downregulated in the H22 tumors of animals treated with Tan ME in a dose-dependent manner. Our results suggest that as a drug delivery system, the ME enhances the antitumor effect of Tan.
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1 

Voiculescu M, Winkler RE, Moscovici M and Neuman MG: Chemotherapies and targeted therapies in advanced hepatocellular carcinoma: from laboratory to clinic. J Gastrointestin Liver Dis. 17:315–322. 2008.PubMed/NCBI

2 

Zhang HT, Luo H, Wu J, et al: Galangin induces apoptosis of hepatocellular carcinoma cells via the mitochondrial pathway. World J Gastroenterol. 16:3377–3384. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Tian HL, Yu T, Xu NN, et al: A novel compound modified from tanshinone inhibits tumor growth in vivo via activation of the intrinsic apoptotic pathway. Cancer Lett. 297:18–30. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Du JR, Li X, Zhang R and Qian ZM: Tanshinone inhibits intimal hyperplasia in the ligated carotid artery in mice. J Ethnopharmacol. 98:319–322. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Kai G, Xu H, Zhou C, et al: Metabolic engineering tanshinone biosynthetic pathway in Salvia miltiorrhiza hairy root cultures. Metab Eng. 13:319–327. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Brown D, Lydon J, McLaughlin M, Stuart-Tilley A, Tyszkowski R and Alper S: Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS). Histochem Cell Biol. 105:261–267. 1996. View Article : Google Scholar : PubMed/NCBI

7 

Su CC and Lin YH: Tanshinone IIA down-regulates the protein expression of ErbB-2 and up-regulates TNF-α in colon cancer cells in vitro and in vivo. Int J Mol Med. 22:847–851. 2008.PubMed/NCBI

8 

Zhou L, Chan WK, Xu N, et al: Tanshinone IIA, an isolated compound from Salvia miltiorrhiza Bunge, induces apoptosis in HeLa cells through mitotic arrest. Life Sci. 83:394–403. 2008.PubMed/NCBI

9 

Han DH, Jin ZH, Jin YZ, Yin XZ, Shen YY and Gao ZG: Thermal reversible microemulsion system for poorly water-soluble YH439 for oral delivery. Chem Pharm Bull (Tokyo). 58:11–15. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Lawrence MJ and Rees GD: Microemulsion-based media as novel drug delivery systems. Adv Drug Deliv Rev. 45:89–121. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Jadhav KR, Shaikh IM, Ambade KW and Kadam VJ: Applications of microemulsion based drug delivery system. Curr Drug Deliv. 3:267–273. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Fan Q, Fan GJ, Yang PM and Zhao JY: Effect of tanshinone microemulsion on reversing MDR in human tumor cells. Zhongguo Zhong Yao Za Zhi. 29:1079–1081. 2004.(In Chinese).

13 

Cibelli M, Fidalgo AR, Terrando N, et al: Role of interleukin-1beta in postoperative cognitive dysfunction. Ann Neurol. 68:360–368. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Yan D, Bao HY, Bau T, Li Y and Kim YH: Antitumor components from Naematoloma fasciculare. J Microbiol Biotechnol. 19:1135–1138. 2009.

15 

Sawada K, Kalam-Azad A, Sakata-Haga H, Lee NS, Jeong YG and Fukui Y: Striking pattern of Purkinje cell loss in cerebellum of an ataxic mutant mouse, tottering. Acta Neurobiol Exp (Warsaw). 69:138–145. 2009.PubMed/NCBI

16 

Nornoo AO, Osborne DW and Chow DS: Cremophor-free intravenous microemulsions for paclitaxel I: formulation, cytotoxicity and hemolysis. Int J Pharm. 349:108–116. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Tsai YH, Hsieh YH, Huang YB, Chang JS, Huang CT and Wu PC: Microemulsions for intravesical delivery of gemcitabine. Chem Pharm Bull (Tokyo). 58:1461–1465. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Debatin KM: Apoptosis pathways in cancer and cancer therapy. Cancer Immunol Immunother. 53:153–159. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Liu XD, Fan RF, Zhang Y, et al: Down-regulation of telomerase activity and activation of caspase-3 are responsible for tanshinone I-induced apoptosis in monocyte leukemia cells in vitro. Int J Mol Sci. 11:2267–2280. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Wang W, Guo QL, You QD, et al: The anticancer activities of wogonin in murine sarcoma S180 both in vitro and in vivo. Biol Pharm Bull. 29:1132–1137. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Kernt M, Neubauer AS, Eibl KH, et al: Minocycline is cytoprotective in human trabecular meshwork cells and optic nerve head astrocytes by increasing expression of XIAP, survivin, and Bcl-2. Clin Ophthalmol. 4:591–604. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Su CC and Lin YH: Tanshinone IIA inhibits human breast cancer cells through increased Bax to Bcl-xL ratios. Int J Mol Med. 22:357–361. 2008.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ma H, Fan Q, Yu J, Xin J and Zhang C: Anticancer activities of tanshinone microemulsion against hepatocellular carcinoma in vitro and in vivo. Mol Med Rep 7: 59-64, 2013.
APA
Ma, H., Fan, Q., Yu, J., Xin, J., & Zhang, C. (2013). Anticancer activities of tanshinone microemulsion against hepatocellular carcinoma in vitro and in vivo. Molecular Medicine Reports, 7, 59-64. https://doi.org/10.3892/mmr.2012.1129
MLA
Ma, H., Fan, Q., Yu, J., Xin, J., Zhang, C."Anticancer activities of tanshinone microemulsion against hepatocellular carcinoma in vitro and in vivo". Molecular Medicine Reports 7.1 (2013): 59-64.
Chicago
Ma, H., Fan, Q., Yu, J., Xin, J., Zhang, C."Anticancer activities of tanshinone microemulsion against hepatocellular carcinoma in vitro and in vivo". Molecular Medicine Reports 7, no. 1 (2013): 59-64. https://doi.org/10.3892/mmr.2012.1129
Copy and paste a formatted citation
x
Spandidos Publications style
Ma H, Fan Q, Yu J, Xin J and Zhang C: Anticancer activities of tanshinone microemulsion against hepatocellular carcinoma in vitro and in vivo. Mol Med Rep 7: 59-64, 2013.
APA
Ma, H., Fan, Q., Yu, J., Xin, J., & Zhang, C. (2013). Anticancer activities of tanshinone microemulsion against hepatocellular carcinoma in vitro and in vivo. Molecular Medicine Reports, 7, 59-64. https://doi.org/10.3892/mmr.2012.1129
MLA
Ma, H., Fan, Q., Yu, J., Xin, J., Zhang, C."Anticancer activities of tanshinone microemulsion against hepatocellular carcinoma in vitro and in vivo". Molecular Medicine Reports 7.1 (2013): 59-64.
Chicago
Ma, H., Fan, Q., Yu, J., Xin, J., Zhang, C."Anticancer activities of tanshinone microemulsion against hepatocellular carcinoma in vitro and in vivo". Molecular Medicine Reports 7, no. 1 (2013): 59-64. https://doi.org/10.3892/mmr.2012.1129
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