Detection of IL-6 by magnetic nanoparticles grown with the assistance of mid-infrared lighting

Jiang,Xiufeng; Zhang,Ye; Miao,Xiaofei; Li,Zenghui; Bao,Zengtao; Wang,Tong

doi:10.3892/mmr.2012.1136

Detection of IL-6 by magnetic nanoparticles grown with the assistance of mid-infrared lighting

Authors:
- Xiufeng Jiang
- Ye Zhang
- Xiaofei Miao
- Zenghui Li
- Zengtao Bao
- Tong Wang
View Affiliations

Affiliations: Department of General Surgery, Wuxi People's Hospital, Wuxi, Jiangsu 214023, P.R. China
Published online on: October 19, 2012 https://doi.org/10.3892/mmr.2012.1136
Pages: 73-76

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Nanomedical systems have attracted considerable attention primarily due to suitability in applications for specific cell selection through biomolecular targeting and rare cell detection enhancement in a diverse, multicellular population. In the present study, magnetic nanoparticles were prepared for use in high accuracy cell sensing. Magnetic nanoparticle growth was assisted by mid-infrared lighting. By this mechanism, a narrow window, estimated to be 2%, was achieved for the dimension distribution of grown nanoparticles. Combined with silicon nanowire (SiNW) transistors, a sensor with ultra high sensitivity for the detection of specific potential low abundance biomarkers has been achieved, which has been specifically used to detect interleukin-6 (IL-6) at extremely low concentrations. A novel biosensor with high sensitivity has been fabricated and utilized in the detection of IL-6 at 75 fM to 50 pM. The system consists of an SiNW transistor and magnetic nanoparticles with even dimension distribution. The novel sensor system is suitable for quantifying IL-6 at low concentrations in protein samples.

View Figures

View References

1	Macy EM, Hayes TE and Tracy RP: Variability in the measurement of C-reactive protein in healthy subjects: implications for reference intervals and epidemiological applications. Clin Chem. 43:52–58. 1997.PubMed/NCBI
2	Engvall E and Perlmann P: Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 8:871–874. 1971. View Article : Google Scholar : PubMed/NCBI
3	Alivisatos P: The use of nanocrystals in biological detection. Nat Biotechnol. 22:47–52. 2004. View Article : Google Scholar : PubMed/NCBI
4	Gao X, Cui Y, Levenson RM, et al: In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol. 22:969–976. 2004. View Article : Google Scholar : PubMed/NCBI
5	Soukka T, Paukkunen J, Harma H, Lonnberg S, Lindroos H and Lovgren T: Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology. Clin Chem. 47:1269–1278. 2001.PubMed/NCBI
6	Nam JM, Thaxton CS and Mirkin CA: Nanoparticle-based bio-bar codes for the ultrasensitive detection of proteins. Science. 301:1884–1886. 2003. View Article : Google Scholar : PubMed/NCBI
7	Wu G, Datar RH, Hansen KM, et al: Bioassay of prostate-specific antigen (PSA) using microcantilevers. Nat Biotechnol. 19:856–860. 2001. View Article : Google Scholar : PubMed/NCBI
8	Chen RJ, Bangsaruntip S, Drouvalakis KA, et al: Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors. Proc Natl Acad Sci USA. 100:4984–4989. 2003. View Article : Google Scholar : PubMed/NCBI
9	Chen RJ, Choi HC, Bangsaruntip S, et al: An investigation of the mechanisms of electronic sensing of protein adsorption on carbon nanotube devices. J Am Chem Soc. 126:1563–1568. 2004. View Article : Google Scholar : PubMed/NCBI
10	Bosch FX, Ribes J, Diaz M and Cleries R: Primary liver cancer: worldwide incidence and trends. Gastroenterology. 127:S5–S16. 2004. View Article : Google Scholar : PubMed/NCBI
11	Scheller J and Rose-John S: Interleukin-6 and its receptor: from bench to bedside. Med Microbiol Immunol. 195:173–183. 2006. View Article : Google Scholar : PubMed/NCBI
12	Pedersen BK and Febbraio MA: Muscle as an endocrine organ: focus on muscle-derived interleukin-6. Physiol Rev. 88:1379–1406. 2008. View Article : Google Scholar : PubMed/NCBI
13	Mora LB, Buettner R, Seigne J, et al: Constitutive activation of Stat3 in human prostate tumors and cell lines: direct inhibition of Stat3 signaling induces apoptosis of prostate cancer cells. Cancer Res. 62:6659–6666. 2002.PubMed/NCBI
14	Bromberg JF, Wrzeszczynska MH, Devgan G, et al: Stat3 as an oncogene. Cell. 98:295–303. 1999. View Article : Google Scholar
15	Swift DC, Gammel JT and Clegg SM: Treatment of compounds and alloys in radiation hydrodynamics simulations of ablative laser loading. Phys Rev E Stat Nonlin Soft Matter Phys. 69:564012004. View Article : Google Scholar : PubMed/NCBI
16	Lee GH, Huh SH, Jeong JW, et al: Structural and magnetic properties of bimetallic FeCo nanoclusters. Korean Phys Soc. 42:367–370. 2003.
17	Wang ZH, Choi CJ, Kim BK, et al: Microstructure and magnetic property of Fe-Co nanoparticles prepared by chemical vapor condensation process. J Alloys Compounds. 351:319–323. 2005. View Article : Google Scholar
18	Dong XL, Zhang ZD, Jin SR and Kim BK: Characterization and magnetic properties of Fe-Co ultrafine particles. J Magn Magn Mater. 210:143–144. 2000. View Article : Google Scholar
19	Basu S and Chakravorty D: Optical properties of nanocomposites with iron core-iron oxide shell structure. J Non Cryst Solids. 352:380–385. 2006. View Article : Google Scholar
20	Ong PL, Mahmood S, Zhang T, et al: Synthesis of cobalt ferrite nanoparticles by pulsed laser deposition in a diffusion cloud chamber. Surf Sci. 254:1909–1914. 2008. View Article : Google Scholar
21	Yanfeng J and Yamin Z: Influence of gold particle size on melting temperature of VLS grown silicon nanowire. J Semicond. 31:120022010. View Article : Google Scholar