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Article

Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells

  • Authors:
    • Liang-Liang Sun
    • Le Zhang
    • Xiang-Lan Meng
    • Feng Zhang
    • Yun Zhao
    • Xin Jin
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, School of Medicine, Xiamen University, Xiamen 361005, Fujian, P.R. China
  • Pages: 110-114
    |
    Published online on: October 22, 2012
       https://doi.org/10.3892/mmr.2012.1137
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Abstract

To investigate the molecular mechanisms of laminar shear stress on the inhibition of apoptosis in endothelial cells, human umbilical vein endothelial cells (HUVECs) were starved in medium containing 2% fetal bovine serum (FBS) and treated with 15 dyne/cm2 shear stress. We confirmed that 15 dyne/cm2 shear stress inhibited the expression of Omi/HtrA2 at the mRNA and protein levels in cultured HUVECs. Furthermore, the release of Omi/HtrA2 from the mitochondria was induced by removal of basic fibroblast growth factor and decrease of FBS in the medium, while shear stress inhibited its release under the same conditions. These results suggest that downregulation of Omi/HtrA2 may contribute to the potent anti-atherosclerotic effect of shear stress by preventing endothelial cells from entering apoptosis.
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1 

Dimmeler S, Haendeler J, Rippman V, Nehls M and Zeiher AM: Shear stress inhibits apoptosis of human endothelial cells. FEBS Lett. 399:71–74. 1996. View Article : Google Scholar : PubMed/NCBI

2 

Haga M, Chen A, Gortler D, Dardik A and Sumpio BE: Shear stress and cyclic strain may suppress apoptosis in endothelial cells by different pathways. Endothelium. 10:149–157. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Ku DN, Giddens DP, Zarins CK and Glagov S: Pulsatile flow and atherosclerosis in the human carotid bifurcation: positive correlation between plaque location and low oscillating shear stress. Arteriosclerosis. 5:293–302. 1985. View Article : Google Scholar

4 

Asakura T and Karino T: Flow patterns and spatial distribution of atherosclerotic lesions in human coronary arteries. Circ Res. 66:1045–1066. 1990. View Article : Google Scholar : PubMed/NCBI

5 

Kaiser D, Freyberg MA and Friedl P: Lack of hemodynamic forces triggers apoptosis in vascular endothelial cells. Biochem Biophys Res Commun. 231:586–590. 1997. View Article : Google Scholar : PubMed/NCBI

6 

Jin X, Mitsumata M, Yamane T and Yoshida Y: Induction of human inhibitor of apoptosis protein-2 by shear stress in endothelial cells. FEBS Letters. 529:286–292. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Jin X, Shu Q, Ling GL and Wang HL: X-linked inhibitor of apoptosis protein is regulated by laminar shear stress in cultured endothelial cells. Chin J Pharmacol Toxicol. 17:328–332. 2003.

8 

Suzuki Y, Imai Y, Nakayama H, Takahashi K, Takio K and Takahashi R: A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol Cell. 8:613–621. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Soustiel JF and Larisch S: Mitochondrial damage: A target for new therapeutic horizons. Neurotherapeutics. 7:13–21. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Pruefer FG, Lizarraga F, Maldonado V and Melendez-Zajgla J: Participation of Omi/Htra2 serine-protease activity in the apoptosis induced by cisplatin on SW480 colon cancer cells. J Chemother. 20:348–354. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Liu QB, Liu LL, Lu YM, Tao RR, Huang JY, Han F and Lou YJ: The induction of reactive oxygen species and loss of mitochondrial Omi/HtrA2 is associated with S-nitrosoglutathione-induced apoptosis in human endothelial cells. Toxicol Appl Pharmacol. 244:374–384. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Kim J, Kim DS, Park MJ, Cho HJ, Zervos AS, Bonventre JV and Park KM: Omi/HtrA2 protease is associated with tubular cell apoptosis and fibrosis induced by unilateral ureteral obstruction. Am J Physiol Renal Physiol. 298:F1332–F1340. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Tricot O, Mallat Z, Heymes C, Belmin J, Leseche G and Tedgui A: Relation between endothelial cell apoptosis and blood flow direction in human atherosclerotic plaques. Circulation. 101:2450–2453. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Bartling B, Tostlebe H, Darmer D, Holtz J, Silber RE and Morawietz H: Shear stress dependent expression of apoptosis regulating genes in endothelial cells. Biochem Biophys Res Commun. 278:740–746. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Dimmeler S, Assmus B, Hermann C, Haendeler J and Zeiher AM: Fluid shear stress stimulates phosphorylation of Akt in human endothelial cells: involvement in suppression of apoptosis. Circ Res. 83:334–341. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Li P, Nijhawan D, Budihardjo I, et al: Cytochrome C and dATP dependent formation of Apaf-1/ caspase-9 complex initiates an apoptotic protease cascade. Cell. 91:479–489. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Hegde R, Srinivasula SM, Zhang Z, et al: Identification of Omi/ HtrA2 as a mitochondrial apoptotic serine protease that disrupts inhibitor of apoptosis protein-caspase interaction. J Biol Chem. 277:432–438. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun L, Zhang L, Meng X, Zhang F, Zhao Y and Jin X: Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells. Mol Med Rep 7: 110-114, 2013.
APA
Sun, L., Zhang, L., Meng, X., Zhang, F., Zhao, Y., & Jin, X. (2013). Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells. Molecular Medicine Reports, 7, 110-114. https://doi.org/10.3892/mmr.2012.1137
MLA
Sun, L., Zhang, L., Meng, X., Zhang, F., Zhao, Y., Jin, X."Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells". Molecular Medicine Reports 7.1 (2013): 110-114.
Chicago
Sun, L., Zhang, L., Meng, X., Zhang, F., Zhao, Y., Jin, X."Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells". Molecular Medicine Reports 7, no. 1 (2013): 110-114. https://doi.org/10.3892/mmr.2012.1137
Copy and paste a formatted citation
x
Spandidos Publications style
Sun L, Zhang L, Meng X, Zhang F, Zhao Y and Jin X: Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells. Mol Med Rep 7: 110-114, 2013.
APA
Sun, L., Zhang, L., Meng, X., Zhang, F., Zhao, Y., & Jin, X. (2013). Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells. Molecular Medicine Reports, 7, 110-114. https://doi.org/10.3892/mmr.2012.1137
MLA
Sun, L., Zhang, L., Meng, X., Zhang, F., Zhao, Y., Jin, X."Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells". Molecular Medicine Reports 7.1 (2013): 110-114.
Chicago
Sun, L., Zhang, L., Meng, X., Zhang, F., Zhao, Y., Jin, X."Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells". Molecular Medicine Reports 7, no. 1 (2013): 110-114. https://doi.org/10.3892/mmr.2012.1137
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