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Article

Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages

  • Authors:
    • Yi Zhong
    • Tingrong Liu
    • Wenyan Lai
    • Ying Tan
    • Di Tian
    • Zhigang Guo
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, P.R. China, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
  • Pages: 242-246
    |
    Published online on: October 22, 2012
       https://doi.org/10.3892/mmr.2012.1138
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Abstract

Monocyte chemoattractant protein 1 (MCP-1) is a CC chemokine which stimulates mononuclear leukocytes and is significant in the pathogenesis of inflammatory disease. Curcumin, extracted from Curcumae longae, has been shown to possess anti-inflammatory activity, in inflammation associated with the induction of MCP-1 expression. However, the underlying mechanisms involved in the effect of curcumin on MCP-1 expression remain unclear. In the current study, we investigated the effect of curcumin on the production of MCP-1 induced by lipopolysaccharide (LPS) in macrophages and the possible mechanisms involved. The results revealed that curcumin decreased MCP-1 production in a concentration-dependent manner and reduced the generation of reactive oxygen species (ROS) induced by LPS in RAW264.7 macrophages. Additionally, zinc protoporphyrin, a heme oxygenase-1 (HO-1) inhibitor, blocked the inhibitory effect of curcumin on the LPS-induced MCP-1 expression. The exposure of cells to curcumin was found to enhance HO-1 expression. Furthermore, additional experiments indicated that the inhibitory effect of curcumin on LPS-induced MCP-1 expression was significantly attenuated in the presence of N-acetylcysteine (an effective ROS scavenger). The results presented in our study suggest that curcumin enhances the expression of HO-1 to reduce the LPS-induced production of ROS, which leads to the inhibition of MCP-1 expression in RAW264.7 macrophages.
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Copy and paste a formatted citation
Spandidos Publications style
Zhong Y, Liu T, Lai W, Tan Y, Tian D and Guo Z: Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages. Mol Med Rep 7: 242-246, 2013.
APA
Zhong, Y., Liu, T., Lai, W., Tan, Y., Tian, D., & Guo, Z. (2013). Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages. Molecular Medicine Reports, 7, 242-246. https://doi.org/10.3892/mmr.2012.1138
MLA
Zhong, Y., Liu, T., Lai, W., Tan, Y., Tian, D., Guo, Z."Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages". Molecular Medicine Reports 7.1 (2013): 242-246.
Chicago
Zhong, Y., Liu, T., Lai, W., Tan, Y., Tian, D., Guo, Z."Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages". Molecular Medicine Reports 7, no. 1 (2013): 242-246. https://doi.org/10.3892/mmr.2012.1138
Copy and paste a formatted citation
x
Spandidos Publications style
Zhong Y, Liu T, Lai W, Tan Y, Tian D and Guo Z: Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages. Mol Med Rep 7: 242-246, 2013.
APA
Zhong, Y., Liu, T., Lai, W., Tan, Y., Tian, D., & Guo, Z. (2013). Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages. Molecular Medicine Reports, 7, 242-246. https://doi.org/10.3892/mmr.2012.1138
MLA
Zhong, Y., Liu, T., Lai, W., Tan, Y., Tian, D., Guo, Z."Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages". Molecular Medicine Reports 7.1 (2013): 242-246.
Chicago
Zhong, Y., Liu, T., Lai, W., Tan, Y., Tian, D., Guo, Z."Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages". Molecular Medicine Reports 7, no. 1 (2013): 242-246. https://doi.org/10.3892/mmr.2012.1138
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