mRNA expression of interleukins and Th1/Th2 imbalance in patients with pulmonary embolism

Duan,Qianglin; Lv,Wei; Wang,Lemin; Gong,Zhu; Wang,Qiang; Song,Haoming; Wang,Hao

doi:10.3892/mmr.2012.1142

January 2013 Volume 7 Issue 1

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January 2013 Volume 7 Issue 1

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Article

mRNA expression of interleukins and Th1/Th2 imbalance in patients with pulmonary embolism

Authors:
- Qianglin Duan
- Wei Lv
- Lemin Wang
- Zhu Gong
- Qiang Wang
- Haoming Song
- Hao Wang
View Affiliations / Copyright

Affiliations: Department of Cardiology, Tongji Hospital of Tongji University, Shanghai 200065, P.R. China
Pages: 332-336
|
Published online on: October 23, 2012

https://doi.org/10.3892/mmr.2012.1142
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Abstract

Few studies have investigated the changes of Th1- and Th2-type cytokines in pulmonary embolism (PE) patients. In this study, the gene expression of interleukins and the balance of Th1- and Th2-type cytokines in the peripheral blood mononuclear cells (PBMCs) of PE patients and controls were investigated. A total of 20 PE patients and 20 gender- and age-matched controls were included in the study. Human cDNA microarray analysis was used to detect the differences in cytokine gene expression between the two groups and a random variance model corrected t-test was used to analyze the statistical data. In comparison with the controls, 12 genes were found to be downregulated, specifically IL1A, IL9, IL17B, IL19, IL23A, IL25 (p<0.05), IL2, IL3, IL13, IL22, IL24 and IL31 (p<0.01), and 2 genes were found to be upregulated, specifically IL10 and IL28A, in the PE patients. The expression levels of IFN-γ and IL2 mRNA in the PE patients were significantly lower than those in the control group (p<0.01), while the IL20 mRNA expression levels were significantly upregulated (p<0.01). We conclude that there are significant differences in interleukin gene expression between the PE patients and the control group. A shift of the Th1/Th2 balance comprising enhanced Th2 activity and reduced Th1 activity in the PE patients is also demonstrated.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

mRNA expression of interleukins and Th1/Th2 imbalance in patients with pulmonary embolism

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