Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January 2013 Volume 7 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January 2013 Volume 7 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effects of probucol on angiotensin II-induced BMP-2 expression in human umbilical vein endothelial cells

  • Authors:
    • Ming Zhang
    • Jian Wang
    • Jing-Hua Liu
    • Shu-Juan Chen
    • Bin Zhen
    • Chang-Hua Wang
    • Hua He
    • Chen-Xi Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Beijing Anzhen Hospital, Capital University of Medical Sciences, Beijing 100029, P.R. China
  • Pages: 177-182
    |
    Published online on: October 24, 2012
       https://doi.org/10.3892/mmr.2012.1145
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Bone morphogenetic protein-2 (BMP-2) participates significantly in vascular development and pathophysiological processes. Angiotensin II (AngII) has been demonstrated to be critical in the initiation and progression of atherosclerosis. However, the effects of AngII on BMP-2 expression and of probucol on the AngII-induced BMP-2 expression in human umbilical vein endothelial cells (HUVECs) are unknown. The aim of our study was to investigate these effects. HUVECs were cultured and stimulated with various agents. The total superoxide dismutase (SOD) activity and the concentrations of malondialdehyde (MDA) and BMP-2 were measured by standard methods. Northern blotting was used to detect the expression of BMP-2 mRNA. The activation of NF-κB in the HUVECs was also determined. The AngII treatment significantly increased BMP-2 expression levels and activated NF-κB. These effects were suppressed by treatment with pyrrolidine dithiocarbamate (PDTC) or probucol. Furthermore, the increased levels of MDA in the conditioned medium and the decrease in the total SOD activity caused by the AngII treatment were reversed by treatment with probucol or PDTC. Probucol downregulated the AngII‑induced BMP-2 expression. These effects of probucol may be mediated by the inhibition of NF-κB activation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Csiszar A, Smith KE, Koller A, Kaley G, Edwards JG and Ungvari Z: Regulation of bone morphogenetic protein-2 expression in endothelial cells: role of nuclear factor-kappaB activation by tumor necrosis factor-alpha, H2O2, and high intravascular pressure. Circulation. 111:2364–2372. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Abedin M, Tintut Y and Demer LL: Vascular calcification: mechanisms and clinical ramifications. Arterioscler Thromb Vasc Biol. 24:1161–1170. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Shanahan CM, Cary NR, Metcalfe JC and Weissberg PL: High expression of genes for calcification-regulating proteins in human atherosclerotic plaques. J Clin Invest. 93:2393–2402. 1994. View Article : Google Scholar : PubMed/NCBI

4 

Parhami F, Boström K, Watson K and Demer LL: Role of molecular regulation in vascular calcification. J Atheroscler Thromb. 3:90–94. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Hruska KA, Mathew S and Saab G: Bone morphogenetic proteins in vascular calcification. Circ Res. 97:105–114. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Boström KI, Jumabay M, Matveyenko A, Nicholas SB and Yao Y: Activation of vascular bone morphogenetic protein signaling in diabetes mellitus. Circ Res. 108:446–457. 2011.PubMed/NCBI

7 

Libby P, Ridker PM and Maseri A: Inflammation and Atherosclerosis. Circulation. 105:1135–1143. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Kranzhöfer R, Schmidt J, Pfeiffer CA, Hagl S, Libby P and Kübler W: Angiotensin induces inflammatory activation of human vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 19:1623–1629. 1999.PubMed/NCBI

9 

Tummala PE, Chen XL, Sundell CL, Laursen JB, Hammes CP, Alexander RW, Harrison DG and Medford RM: Angiotensin II induces vascular cell adhesion molecule-1 expression in rat vasculature: A potential link between the renin-angiotensin system and atherosclerosis. Circulation. 100:1223–1229. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Yung LM, Wong WT, Tian XY, Leung FP, Yung LH, Chen ZY, Yao X, Lau CW and Huang Y: Inhibition of renin-angiotensin system reverses endothelial dysfunction and oxidative stress in estrogen deficient rats. PLoS One. 29:e174372011. View Article : Google Scholar : PubMed/NCBI

11 

Hernández-Presa M, Bustos C, Ortego M, Tuñon J, Renedo G, Ruiz-Ortega M and Egido J: Angiotensin-converting enzyme inhibition prevents arterial nuclear factor-kappaB activation, monocyte chemoattractant protein-1 expression, and macrophage infiltration in a rabbit model of early accelerated atherosclerosis. Circulation. 95:1532–1541. 1997.

12 

Sawayama Y, Shimizu C, Maeda N, Tatsukawa M, Kinukawa N, Koyanagi S, Kashiwagi S and Hayashi J: Effects of probucol and pravastatin on common carotid atherosclerosis in patients with asymptomatic hypercholesterolemia: Fukuoka Atherosclerosis Trial (FAST). J Am Coll Cardiol. 39:610–616. 2002. View Article : Google Scholar

13 

Mackness B, Durrington PN and Mackness MI: Lack of protection against oxidative modification of LDL by avian HDL. Biochem Biophys Res Commun. 247:443–446. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Lowry OH, Rosebrough NJ, Farr AL and Randall RJ: Protein measurement with the folin-phenol reagent. J Biol Chem. 193:265–275. 1951.PubMed/NCBI

15 

Oyama VI and Eagle H: Measurement of cell growth in tissue culture with a phenol reagent (Folin-Ciocalteau). Proceedings of the Society of Experimental Biology and Medicine. 91:305–307. 1956. View Article : Google Scholar : PubMed/NCBI

16 

Zhang H and Bradley A: Mice deficient for BMP2 are nonviable and have defects in amnion/chorion and cardiac development. Development. 122:2977–2986. 1996.PubMed/NCBI

17 

Suzuki Y, Ruiz-Ortega M, Lorenzo O, Ruperez M, Esteban V and Egido J: Inflammation and angiotensin II. Int J Biochem Cell Biol. 35:881–900. 2003. View Article : Google Scholar

18 

Durante A, Peretto G, Laricchia A, Ancona F, Spartera M, Mangieri A and Cianflone D: Role of the renin-angiotensin-aldosterone system in the pathogenesis of atherosclerosis. Curr Pharm Des. 18:981–1004. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Tardif JC, Grégoire J and L’Allier PL: Prevention of restenosis with antioxidants: mechanisms and implications. Am J Cardiovasc Drugs. 2:323–334. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Buckley MMT, Goa KL, Price AH and Brogden RN: Probucol: a reappraisal of its pharmacological properties and therapeutic use in hypercholestecmia. Drugs. 37:761–800. 1989. View Article : Google Scholar : PubMed/NCBI

21 

Tardif JC, Grégoire J, Schwartz L, Title L, Laramée L, Reeves F, Lespérance J, Bourassa MG, L’Allier PL, Glass M, Lambert J and Guertin MC; Canadian Antioxidant Restenosis Trial (CART-1) Investigators. Effects of AGI-1067 and probucol after percutaneous coronary interventions. Circulation. 107:552–558. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Kasai T, Miyauchi K, Kubota N, Kajimoto K, Amano A and Daida H: Probucol therapy improves long-term (>10-year) survival after complete revascularization: a propensity analysis. Atherosclerosis. 220:463–469. 2012.

23 

Traverso N, Menini S, Maineri EP, Patriarca S, Odetti P, Cottalasso D, Marinari UM and Pronzato MA: Malondialdehyde, a lipoperoxidation-derived aldehyde, can bring about secondary oxidative damage to proteins. J Gerontol A Biol Sci Med Sci. 59:B890–B895. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Memişoğulari R and Bakan E: Levels of ceruloplasmin, transferring, and lipid peroxidation in serum of patients with Type 2 diabetes mellitus. J Diabetes Complications. 18:193–197. 2004.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang M, Wang J, Liu J, Chen S, Zhen B, Wang C, He H and Jiang C: Effects of probucol on angiotensin II-induced BMP-2 expression in human umbilical vein endothelial cells. Mol Med Rep 7: 177-182, 2013.
APA
Zhang, M., Wang, J., Liu, J., Chen, S., Zhen, B., Wang, C. ... Jiang, C. (2013). Effects of probucol on angiotensin II-induced BMP-2 expression in human umbilical vein endothelial cells. Molecular Medicine Reports, 7, 177-182. https://doi.org/10.3892/mmr.2012.1145
MLA
Zhang, M., Wang, J., Liu, J., Chen, S., Zhen, B., Wang, C., He, H., Jiang, C."Effects of probucol on angiotensin II-induced BMP-2 expression in human umbilical vein endothelial cells". Molecular Medicine Reports 7.1 (2013): 177-182.
Chicago
Zhang, M., Wang, J., Liu, J., Chen, S., Zhen, B., Wang, C., He, H., Jiang, C."Effects of probucol on angiotensin II-induced BMP-2 expression in human umbilical vein endothelial cells". Molecular Medicine Reports 7, no. 1 (2013): 177-182. https://doi.org/10.3892/mmr.2012.1145
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang M, Wang J, Liu J, Chen S, Zhen B, Wang C, He H and Jiang C: Effects of probucol on angiotensin II-induced BMP-2 expression in human umbilical vein endothelial cells. Mol Med Rep 7: 177-182, 2013.
APA
Zhang, M., Wang, J., Liu, J., Chen, S., Zhen, B., Wang, C. ... Jiang, C. (2013). Effects of probucol on angiotensin II-induced BMP-2 expression in human umbilical vein endothelial cells. Molecular Medicine Reports, 7, 177-182. https://doi.org/10.3892/mmr.2012.1145
MLA
Zhang, M., Wang, J., Liu, J., Chen, S., Zhen, B., Wang, C., He, H., Jiang, C."Effects of probucol on angiotensin II-induced BMP-2 expression in human umbilical vein endothelial cells". Molecular Medicine Reports 7.1 (2013): 177-182.
Chicago
Zhang, M., Wang, J., Liu, J., Chen, S., Zhen, B., Wang, C., He, H., Jiang, C."Effects of probucol on angiotensin II-induced BMP-2 expression in human umbilical vein endothelial cells". Molecular Medicine Reports 7, no. 1 (2013): 177-182. https://doi.org/10.3892/mmr.2012.1145
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team