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Article

The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts

  • Authors:
    • Qing Cao
    • Leren Tao
    • Mengfang Liu
    • Meng Yin
    • Kun Sun
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Shanghai Children's Medical Center Affiliated to Shanghai Jiaotong University, Shanghai 200127, P.R. China, Institute of Cryogenic Technology and Frozen Foods, Shanghai University of Technology, Shanghai 200093, P.R. China, Department of Pediatric Cardiothoracic Surgery, Shanghai Children's Medical Center Affiliated to Shanghai Jiaotong University, Shanghai 200127, P.R. China, Department of Pediatric Cardiology, Xinhua Hospital Affiliated to Shanghai Jiatong University, Shanghai 200092, P.R. China
  • Pages: 144-148
    |
    Published online on: October 24, 2012
       https://doi.org/10.3892/mmr.2012.1146
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Abstract

Vacuum freeze-dried aorta is a satisfactory material for blood vessel grafting. Previous studies have focused on immunity, however, vacuum freeze-drying is a complicated process of heat and mass transfer, and adopting a programmed cooling process may more completely preserve the mechanical properties of the blood vessels. Irradiation, as a method of removing pathogens, lowers the antigenic activity of the blood vessels. In our study, vacuum freeze-drying combined with radiation was used as a treatment for porcine aorta prior to grafting, aimed at deactivating endogenous retrovirus, shielding masses of endothelial cells and lowering the immunogenicity of the blood vessels. As for the mechanical properties, compared with normal aorta, the maximum axial tensile stress (ATS) decreased by 20%, the maximum circumferential tensile stress (CTS) increased by 30% and the maximum puncture stress (PT) decreased by 20%. Our results revealed that 2 months after of grafting, the host cells had migrated into the graft tissue and propagated to initiate endothelialization, the inflammatory reaction was abated and the PT had returned to normal levels.
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Copy and paste a formatted citation
Spandidos Publications style
Cao Q, Tao L, Liu M, Yin M and Sun K: The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts. Mol Med Rep 7: 144-148, 2013.
APA
Cao, Q., Tao, L., Liu, M., Yin, M., & Sun, K. (2013). The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts. Molecular Medicine Reports, 7, 144-148. https://doi.org/10.3892/mmr.2012.1146
MLA
Cao, Q., Tao, L., Liu, M., Yin, M., Sun, K."The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts". Molecular Medicine Reports 7.1 (2013): 144-148.
Chicago
Cao, Q., Tao, L., Liu, M., Yin, M., Sun, K."The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts". Molecular Medicine Reports 7, no. 1 (2013): 144-148. https://doi.org/10.3892/mmr.2012.1146
Copy and paste a formatted citation
x
Spandidos Publications style
Cao Q, Tao L, Liu M, Yin M and Sun K: The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts. Mol Med Rep 7: 144-148, 2013.
APA
Cao, Q., Tao, L., Liu, M., Yin, M., & Sun, K. (2013). The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts. Molecular Medicine Reports, 7, 144-148. https://doi.org/10.3892/mmr.2012.1146
MLA
Cao, Q., Tao, L., Liu, M., Yin, M., Sun, K."The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts". Molecular Medicine Reports 7.1 (2013): 144-148.
Chicago
Cao, Q., Tao, L., Liu, M., Yin, M., Sun, K."The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts". Molecular Medicine Reports 7, no. 1 (2013): 144-148. https://doi.org/10.3892/mmr.2012.1146
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