MicroRNA-125b expression in gastric adenocarcinoma and its effect on the proliferation of gastric cancer cells

Yang,Zhao-Xu; Lu,Cheng-Yi; Yang,Yan-Ling; Dou,Ke-Feng; Tao,Kai-Shan

doi:10.3892/mmr.2012.1156

January 2013 Volume 7 Issue 1

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January 2013 Volume 7 Issue 1

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Article

MicroRNA-125b expression in gastric adenocarcinoma and its effect on the proliferation of gastric cancer cells

Authors:
- Zhao-Xu Yang
- Cheng-Yi Lu
- Yan-Ling Yang
- Ke-Feng Dou
- Kai-Shan Tao
View Affiliations / Copyright

Affiliations: Department of Hepatobiliary Surgery, Xijing Hospital of the Fourth Military Medical University, Xi'an 710032, P.R. China, Department of Information, Tangdu Hospital of the Fourth Military Medical University, Xi'an 710038, P.R. China
Pages: 229-232
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Published online on: October 26, 2012

https://doi.org/10.3892/mmr.2012.1156
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Abstract

MicroRNAs exert regulatory effects on a number of genes, thereby contributing to both physiological and pathological processes. The functions of microRNAs in tumorigenesis are becoming increasingly clear. In the present study, we investigated the role of microRNA-125b (miR‑125b), previously implicated in prostate and breast cancer, in gastric cancer, particularly regarding proliferation and apoptosis of gastric cancer cells. The expression of miR‑125b was measured in 50 samples of gastric cancer tissues and corresponding para-cancerous tissues by real-time PCR. The levels of miR‑125b expression in the gastric cancer tissues were significantly higher compared to the adjacent normal tissues (P<0.05). To begin to understand how the increased expression of miR‑125b may promote gastric cancer, the miR‑125b mimic was transfected into the gastric cancer cell line, HGC‑27, for the determination of proliferation (CCK8) and apoptosis (Annexin V) by flow cytometry. The results demonstrated that the proliferation significantly increased and apoptosis significantly decreased in the HGC‑27 cells following transfection with the miR‑125b mimic, compared to the untreated and scramble‑treated controls (P<0.05). Thus, miR‑125b may act as an oncogene in gastric cancer by dysregulating gastric cell proliferation and apoptosis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

MicroRNA-125b expression in gastric adenocarcinoma and its effect on the proliferation of gastric cancer cells

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