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Article

Inhalation of carbon monoxide following resuscitation ameliorates hemorrhagic shock-induced lung injury

  • Authors:
    • Susumu Kawanishi
    • Toru Takahashi
    • Hiroshi Morimatsu
    • Hiroko Shimizu
    • Emiko Omori
    • Kenji Sato
    • Masaki Matsumi
    • Shigeru Maeda
    • Atsunori Nakao
    • Kiyoshi Morita
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology and Resuscitology, Okayama University Medical School, Okayama 700‑8558, Japan, Department of Dental Anesthesiology, Okayama University Dental School, Okayama 700-8558, Japan, Department of Emergency and Critical Care Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan
  • Pages: 3-10
    |
    Published online on: November 8, 2012
       https://doi.org/10.3892/mmr.2012.1173
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Abstract

Even after successful resuscitation, hemorrhagic shock frequently causes pulmonary inflammation that induces acute lung injury (ALI). We previously demonstrated that when CO is inhaled at a low concentration both prior to and following hemorrhagic shock and resuscitation (HSR) it ameliorates HSR-induced ALI in rats due to its anti-inflammatory effects. In the present study, we administered CO to the same model of ALI only after resuscitation and examined whether it exerted a therapeutic effect without adverse events on HSR-induced ALI, since treatment of animals with CO prior to HSR did not prevent lung injury. HSR were induced by bleeding animals to achieve a mean arterial pressure of 30 mmHg for 1 h followed by resuscitation with the removed blood. HSR resulted in the upregulation of inflammatory gene expression and increased the rate of apoptotic cell death in the lungs. This was determined from an observed increase in the number of cells positive for transferase-mediated dUTP-fluorescein isothiocyanate (FITC), nick-end labeling staining and activated caspase-3. HSR also resulted in prominent histopathological damage, including congestion, edema, cellular infiltration and hemorrhage. By contrast, CO inhalation for 3 h following resuscitation significantly ameliorated these inflammatory events, demonstrated by reduced histological damage, inflammatory mediators and apoptotic cell death. The protective effects of CO against lung injury were notably associated with an increase in the protein expression level of peroxisome proliferator-activated receptor (PPAR)-γ, an anti-inflammatory transcriptional regulator in the lung. Moreover, CO inhalation did not affect the hemodynamic status or tissue oxygenation during HSR. These findings suggest that inhalation of CO at a low concentration exerts a potent therapeutic effect against HSR-induced ALI and attenuates the inflammatory cascade by increasing PPAR-γ protein expression.
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Copy and paste a formatted citation
Spandidos Publications style
Kawanishi S, Takahashi T, Morimatsu H, Shimizu H, Omori E, Sato K, Matsumi M, Maeda S, Nakao A, Morita K, Morita K, et al: Inhalation of carbon monoxide following resuscitation ameliorates hemorrhagic shock-induced lung injury. Mol Med Rep 7: 3-10, 2013.
APA
Kawanishi, S., Takahashi, T., Morimatsu, H., Shimizu, H., Omori, E., Sato, K. ... Morita, K. (2013). Inhalation of carbon monoxide following resuscitation ameliorates hemorrhagic shock-induced lung injury. Molecular Medicine Reports, 7, 3-10. https://doi.org/10.3892/mmr.2012.1173
MLA
Kawanishi, S., Takahashi, T., Morimatsu, H., Shimizu, H., Omori, E., Sato, K., Matsumi, M., Maeda, S., Nakao, A., Morita, K."Inhalation of carbon monoxide following resuscitation ameliorates hemorrhagic shock-induced lung injury". Molecular Medicine Reports 7.1 (2013): 3-10.
Chicago
Kawanishi, S., Takahashi, T., Morimatsu, H., Shimizu, H., Omori, E., Sato, K., Matsumi, M., Maeda, S., Nakao, A., Morita, K."Inhalation of carbon monoxide following resuscitation ameliorates hemorrhagic shock-induced lung injury". Molecular Medicine Reports 7, no. 1 (2013): 3-10. https://doi.org/10.3892/mmr.2012.1173
Copy and paste a formatted citation
x
Spandidos Publications style
Kawanishi S, Takahashi T, Morimatsu H, Shimizu H, Omori E, Sato K, Matsumi M, Maeda S, Nakao A, Morita K, Morita K, et al: Inhalation of carbon monoxide following resuscitation ameliorates hemorrhagic shock-induced lung injury. Mol Med Rep 7: 3-10, 2013.
APA
Kawanishi, S., Takahashi, T., Morimatsu, H., Shimizu, H., Omori, E., Sato, K. ... Morita, K. (2013). Inhalation of carbon monoxide following resuscitation ameliorates hemorrhagic shock-induced lung injury. Molecular Medicine Reports, 7, 3-10. https://doi.org/10.3892/mmr.2012.1173
MLA
Kawanishi, S., Takahashi, T., Morimatsu, H., Shimizu, H., Omori, E., Sato, K., Matsumi, M., Maeda, S., Nakao, A., Morita, K."Inhalation of carbon monoxide following resuscitation ameliorates hemorrhagic shock-induced lung injury". Molecular Medicine Reports 7.1 (2013): 3-10.
Chicago
Kawanishi, S., Takahashi, T., Morimatsu, H., Shimizu, H., Omori, E., Sato, K., Matsumi, M., Maeda, S., Nakao, A., Morita, K."Inhalation of carbon monoxide following resuscitation ameliorates hemorrhagic shock-induced lung injury". Molecular Medicine Reports 7, no. 1 (2013): 3-10. https://doi.org/10.3892/mmr.2012.1173
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