Inhibitory effects of Akebia quinata ethanol extract on TNF-α-mediated vascular inflammation in human aortic smooth muscle cells

Koo,Hyun  Jung; Sung,Yoon   Young; Kim,Ho   Kyoung

doi:10.3892/mmr.2012.1193

Inhibitory effects of Akebia quinata ethanol extract on TNF-α-mediated vascular inflammation in human aortic smooth muscle cells

Authors:
- Hyun Jung Koo
- Yoon Young Sung
- Ho Kyoung Kim
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Affiliations: Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea
Published online on: November 21, 2012 https://doi.org/10.3892/mmr.2012.1193
Pages: 379-383

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Abstract

Atherosclerosis is a chronic inflammatory disease of the arterial wall. The expression of adhesion molecules in aortic smooth muscle cells facilitates the accumulation of transmigrated leukocytes within the atherosclerotic vascular wall. The stem of Akebia quinata (A. quinata) has previously been used as a crude drug for treating urinary disorders and inflammatory disease in Korea, China and Japan. In the present study, we investigated the effect of an A. quinata ethanol extract (AQEE) on the expression of adhesion molecules and cyclooxygenase-2 (COX‑2) in tumor necrosis factor α (TNF-α)-induced human aortic smooth muscle cells (HASMCs). The results of the present study demonstrated that AQEE attenuated intercellular adhesion molecule 1, E‑selectin and COX‑2 expression in TNF‑α-stimulated HASMCs and inhibited THP-1 cell adhesion to activated HASMCs. Furthermore, AQEE suppressed the TNF‑α induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and reduced nuclear factor κB (NF-κB; p65) nuclear translocation. The present study demonstrated that AQEE possesses anti‑inflammatory properties and regulates TNF-α-induced expression of adhesion molecules by inhibition of the p38 MAPK/NF-κB signaling pathway.

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1	Lusis AJ: Atherosclerosis. Nature. 407:233–241. 2000. View Article : Google Scholar : PubMed/NCBI
2	Ross R: Atherosclerosis - an inflammatory disease. N Engl J Med. 340:115–126. 1999. View Article : Google Scholar
3	Bevilacqua MP, Nelson RM, Mannori G and Cecconi O: Endothelial-leukocyte adhesion molecules in human disease. Annu Rev Med. 45:361–378. 1994. View Article : Google Scholar : PubMed/NCBI
4	Bobryshev YV, Lord RS, Rainer SP and Munro VF: VCAM-1 expression and network of VCAM-1 positive vascular dendritic cells in advanced atherosclerotic lesions of carotid arteries and aortas. Acta Histochem. 98:185–194. 1996. View Article : Google Scholar : PubMed/NCBI
5	O'Brien KD, McDonald TO, Chait A, Allen MD and Alpers CE: Neovascular expression of E-selectin, intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in human atherosclerosis and their relation to intimal leukocyte content. Circulation. 93:672–682. 1996. View Article : Google Scholar
6	O'Brien KD, Allen MD, McDonald TO, Chait A, Harlan JM, Fishbein D, McCarty J, Ferguson M, Hudkins K and Benjamin CD: Vascular cell adhesion molecule-1 is expressed in human coronary atherosclerotic plaques. Implications for the mode of progression of advanced coronary atherosclerosis. J Clin Invest. 92:945–951. 1993. View Article : Google Scholar : PubMed/NCBI
7	Owens GK, Kumar MS and Wamhoff BR: Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol Rev. 84:767–801. 2004. View Article : Google Scholar : PubMed/NCBI
8	Falk E: Pathogenesis of atherosclerosis. J Am Coll Cardiol. 47(Suppl 8): C7–C12. 2006. View Article : Google Scholar
9	Braun M, Pietsch P, Schrör K, Baumann G and Felix SB: Cellular adhesion molecules on vascular smooth muscle cells. Cardiovasc Res. 41:395–401. 1999. View Article : Google Scholar : PubMed/NCBI
10	Jang Y, Lincoff AM, Plow EF and Topol EJ: Cell adhesion molecules in coronary artery disease. J Am Coll Cardiol. 24:1591–1601. 1994. View Article : Google Scholar : PubMed/NCBI
11	Davies MJ, Gordon JL, Gearing AJ, Pigott R, Woolf N, Katz D and Kyriakopoulos A: The expression of the adhesion molecules ICAM-1, VCAM-1, PECAM and E-selectin in human atherosclerosis. J Pathol. 171:223–229. 1993. View Article : Google Scholar : PubMed/NCBI
12	Libby P and Li H: Vascular cell adhesion molecule-1 and smooth muscle cell activation during atherogenesis. J Clin Invest. 92:538–539. 1993. View Article : Google Scholar : PubMed/NCBI
13	Yu Printseva O, Peclo MM and Gown AM: Various cell types in human atherosclerotic lesions express ICAM-1. Further immunocytochemical and immunochemical studies employing monoclonal antibody 10F3. Am J Pathol. 140:889–896. 1992.
14	Huo Y and Ley K: Adhesion molecules and atherogenesis. Acta Physiol Scand. 173:35–43. 2001. View Article : Google Scholar : PubMed/NCBI
15	Kitaoka F, Kakiuchi N, Long C, Itoga M, Mitsue A, Mouri C and Mikage M: Molecular characterization of akebia plants and the derived traditional herbal medicine. Biol Pharm Bull. 32:665–670. 2009. View Article : Google Scholar : PubMed/NCBI
16	Mimaki Y, Doi S, Kuroda M and Yokosuka A: Triterpene glycosides from the stems of Akebia quinata. Chem Pharm Bull. 55:1319–1324. 2007. View Article : Google Scholar : PubMed/NCBI
17	Choi J, Jung HJ, Lee KT and Park HJ: Antinociceptive and anti-inflammatory effects of the saponin and sapogenins obtained from the stem of Akebia quinata. J Med Food. 8:78–85. 2005. View Article : Google Scholar : PubMed/NCBI
18	Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T and Tada K: Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer. 26:171–176. 1980. View Article : Google Scholar : PubMed/NCBI
19	Chen C, Chou C, Sun Y and Huang W: Tumor necrosis factor alpha-induced activation of downstream NF-kappaB site of the promoter mediates epithelial ICAM-1 expression and monocyte adhesion. Involvement of PKCalpha, tyrosine kinase and IKK2, but not MAPKs, pathway. Cell Signal. 13:543–553. 2001. View Article : Google Scholar
20	Collins T, Read MA, Neish AS, Whitley MZ, Thanos D and Maniatis T: Transcriptional regulation of endothelial cell adhesion molecules: NF-kappa B and cytokine-inducible enhancers. FASEB J. 9:899–909. 1995.PubMed/NCBI
21	Ju JW, Kim SJ, Jun CD and Chun JS: p38 kinase and c-Jun N-terminal kinase oppositely regulates tumor necrosis factor alpha-induced vascular cell adhesion molecule-1 expression and cell adhesion in chondrosarcoma cells. IUBMB Life. 54:293–299. 2002. View Article : Google Scholar
22	Ho AW, Wong CK and Lam CW: Tumor necrosis factor-alpha up-regulates the expression of CCL2 and adhesion molecules of human proximal tubular epithelial cells through MAPK signaling pathways. Immunobiology. 213:533–544. 2008. View Article : Google Scholar : PubMed/NCBI
23	Brasier AR: The nuclear factor-kappaB-interleukin-6 signalling pathway mediating vascular inflammation. Cardiovasc Res. 86:211–218. 2010. View Article : Google Scholar : PubMed/NCBI
24	Pahl HL: Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene. 18:6853–6866. 1999. View Article : Google Scholar : PubMed/NCBI
25	Waddick KG and Uckun FM: Innovative treatment programs against cancer: II. Nuclear factor-kappaB (NF-kappaB) as a molecular target. Biochem Pharmacol. 57:9–17. 1999. View Article : Google Scholar : PubMed/NCBI