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Article

Dexamethasone induces rapid promotion of norepinephrine‑mediated vascular smooth muscle cell contraction

  • Authors:
    • Ting Zhang
    • Wen‑Lei Shi
    • Jeffrey G. Tasker
    • Jiang‑Rui Zhou
    • Yun‑Li Peng
    • Chao‑Yu Miao
    • Yong‑Ji Yang
    • Chun‑Lei Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Nautical Medicine and Laboratory of Stress Medicine, Second Military Medical University, Shanghai 200433, P.R. China, Department of Nautical Medicine and Laboratory of Stress Medicine, Second Military Medical University, Shanghai 200433, P.R. China, Neurobiology Division, Department of Cell and Molecular Biology, Tulane University, New Orleans, LA 70118, USA, Department of Pharmacology, Second Military Medical University, Shanghai 200433, P.R. China, Department of Biophysics, Second Military Medical University, Shanghai 200433, P.R. China
  • Pages: 549-554
    |
    Published online on: November 22, 2012
       https://doi.org/10.3892/mmr.2012.1196
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Abstract

The aim of the present study was to identify the rapid effect of dexamethasone (Dex) on norepinephrine (NE)‑mediated contraction of vascular smooth muscle cells (VSMCs) and to establish the underlying mechanism(s). Rat VSMCs were preincubated with lipopolysaccharide to simulate acute septic shock. Myosin light chain (MLC20) phosphorylation of VSMCs was detected by western blot analysis to observe the effects of Dex on NE‑mediated contraction. Activation of the RhoA/ RhoA kinase (ROCK), extracellular signal‑regulated kinase (ERK) and p38 signaling pathways was detected by western blot analysis to explore the mechanism. It was identified that Dex rapidly promoted NE‑induced phosphorylation of MLC20 in VSMCs and this effect may be non‑genomic. The RhoA/ROCK, ERK and p38 pathways were demonstrated to be important for the rapid effect of Dex‑induced promotion of NE‑mediated contraction in VSMCs. The present results indicate that Dex may rapidly reverse the hyporeactivity of vasoconstriction to NE in vitro and this effect may be mediated by specific non‑genomic mechanisms through increased activation of the RhoA/ROCK, ERK and p38 signaling pathways.
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View References

1 

Somlyo AP and Somlyo AV: Signal transduction and regulation in smooth muscle. Nature. 372:231–236. 1994. View Article : Google Scholar : PubMed/NCBI

2 

Kamm KE and Stull JT: The function of myosin and myosin light chain kinase phosphorylation in smooth muscle. Annu Rev Pharmacol Toxicol. 25:593–620. 1985. View Article : Google Scholar : PubMed/NCBI

3 

Wickman G, Lan C and Vollrath B: Functional roles of the rho/rho kinase pathway and protein kinase C in the relevance regulation of cerebrovascular constriction mediated by hemoglobin: relevance to subarachnoid hemorrhage and vasospasm. Circ Res. 92:809–816. 2003. View Article : Google Scholar

4 

Ito M, Nakano T, Erdodi F and Hartshorne DJ: Myosin phosphatase: structure, regulation and function. Mol Cell Biochem. 259:197–209. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Somlyo AP and Somlyo AV: Signal transduction by G-proteins, rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II. J Physiol. 522:177–185. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Hedges JC, Oxhorn BC, Carty M, Adam LP, Yamboliev IA and Gerthoffer WT: Phosphorylation of caldesmon by ERK MAP kinases in smooth muscle. Am J Physiol Cell Physiol. 278:718–726. 2000.PubMed/NCBI

7 

Yamboliev IA, Hedges JC, Mutnick JL, Adam LP and Gerthoffer WT: Evidence for modulation of smooth muscle force by the p38 MAP kinase/HSP27 pathway. Am J Physiol Heart Circ Physiol. 278:1899–1907. 2000.PubMed/NCBI

8 

Gorenne I, Su X and Moreland RS: Caldesmon phosphorylation is catalyzed by two kinases in permeabilized and intact vascular smooth muscle. J Cell Physiol. 198:461–469. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Zhou J, Li M, Liu L, Sheng CQ, Li Z, Wang Y, Zhou JR, Jiang ZP, Chen YZ and Jiang CL: A novel strategy for development of glucocorticoids through non-genomic mechanism. Cell Mol Life Sci. 9:1–10. 2010.

10 

Zhou J, Liu DF, Liu C, Kang ZM, Shen XH, Chen YZ, Xu T and Jiang C: Glucocorticoids inhibit degranulation of mast cells in allergic asthma via nongenomic mechanism. Allergy. 63:1177–1185. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Sun HW, Miao CY, Liu L, Zhou J, Su DF, Wang YX and Jiang CL: Rapid inhibitory effect of glucocorticoids on airway smooth muscle contractions in guinea pigs. Steriods. 71:154–159. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Liu L, Wang YX, Zhou J, Long F, Sun HW, Liu Y, Chen YZ and Jiang CL: Rapid nongenomic inhibitory effects of glucocorticoids on human neutrophil degranulation. Inflam Res. 54:37–41. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Yang S and Zhang L: Glucocorticoids and vascular reactivity. Curr Vasc Pharmacol. 2:1–12. 2004. View Article : Google Scholar

14 

Mansart A, Bollaert PE, Giummelly P, Capdeville-Atkinson C and Atkinson J: Effects of dexamethasone and L-canavanine on the intracellular calcium-contraction relation of the rat tail artery during septic shock. Am J Physiol Heart Circ Physiol. 291:H1177–H1182. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Takakura K, Xiaohong W, Takeuchi K, Yasuda Y and Fukuda S: Deactivation of norepinephrine by peroxynitrite as a new pathogenesis in the hypotension of septic shock. Anesthesiology. 98:928–934. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Jeon SB, Jin F, Kim JI, Kim SH, Suk K, Chae SC, Jun JE, Park WH and Kim IK: A role for rho kinase in vascular contraction evoked by sodium fluoride. Biochem Biophys Res Commun. 343:27–33. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Somlyo AP and Somlyo AV: Ca2+ sensitivity of smooth muscle and nonmuscle myosin: modulated by G proteins, kinases andmyosin phosphatase. Physiol Rev. 83:1325–1358. 2003.

18 

Mansart A, Bollaert PE, Seguin C, Levy B, Longrois D and Mallie JP: Hemodynamic effects of early versus late glucocorticoid administration in experimental septic shock. Shock. 19:38–44. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Zaloga GP and Marik P: Hypothalamic-pituitary-adrenal insufficiency. Crit Care Clin. 17:25–41. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Annane D, Bellissant E, Sebille V, Lesieur O, Mathieu B, Raphael JC and Gajdos P: Impaired pressor sensitivity to noradrenaline in septic shock patients with and without impaired adrenal function reserve. Br J Clin Pharmacol. 46:589–597. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Hilgers RH and Webb RC: Molecular aspects of arterial smooth muscle contraction: focus on Rho. Exp Biol Med. 230:829–835. 2005.PubMed/NCBI

22 

Ihara E, Moffat L, Ostrander J, Walsh MP and Macdonald JA: Characterization of protein kinase pathways responsible for Ca2+ sensitization in rat ileal longitudinal smooth muscle. Am J Physiol Gastrointest Liver Physiol. 293:699–710. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Loirand G, Guerin P and Pacaud P: Rho kinases in cardiovascular physiology and pathophysiology. Circ Res. 98:322–334. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Noma K, Oyama N and Liao JK: Physiological role of ROCKs in the cardiovascular system. Am J Physiol Cell Physiol. 290:C661–C668. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Shimokawa H and Takeshita A: Rho-kinase is an important therapeutic target in cardiovascular medicine. Arterioscler Thromb Vasc Biol. 25:1767–1775. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Hirokazu T, Hiroki C, Tadashi I, Jun K, Haruhiko M, Yoshisato T, Miyako T, Kazuhiko Y, Masaki N and Hisashi S: Diverse activation states of RhoA in human lung cancer cells: contribution of G protein coupled receptors. Int J Oncol. 30:709–715. 2007.PubMed/NCBI

27 

Bellizzi A, Mangia A, Chiriatti A, Petroni S, Quaranta M, Schittulli F, Malfettone A, Cardone RA, Paradiso A and Reshkin SJ: RhoA protein expression in primary breast cancers and matched lymphocytes is associated with progression of the disease. Int J Mol Med. 22:25–31. 2008.PubMed/NCBI

28 

Baek I, Jeon SB, Kim J, Seok YM, Song MJ, Chae SC, Jun JE, Park WH and Kim IK: A role for Rho-kinase in Ca2+ independent contractions induced by phorbol-12,13-dibutyrate. Clin Exp Pharmacol Physiol. 36:256–261. 2009.

29 

Li T, Liu L, Liu J, Ming J, Xu J, Yang G and Zhang Y: Mechnisms of Rho kinase regulation of vascular reactivity following hemorrhagic shock in rats. Shock. 29:65–70. 2008.PubMed/NCBI

30 

Chiba Y, Goto K, Hirahara M, Sakai H and Misawa M: Glucocorticoids ameliorate antigen-induced bronchial smooth muscle hyperresponsiveness by inhibiting upregulation of RhoA in rats. J Pharmacol Sci. 106:615–625. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Berridge MJ: Smooth muscle cell calcium activation mechanisms. J Physiol. 586:5047–5061. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Tseng S, Kim R, Kim T, Morgan KG and Hai CM: F-actin disruption attenuates agonist-induced [Ca2+], myosin phosphorylation and force in smooth muscle. Am J Physiol. 41:1960–1967. 1997.PubMed/NCBI

33 

Tang DC, Xiang JZ and Lu WT: Calponin: a new regulatory protein for smooth muscle contraction. Prog Biochem Biophys. 23:325–329. 1996.

34 

Matrougui K, Tanko LB, Loufrani L, Gorny D, Levy BI, Tedgui A and Henrion D: Involvement of rho-kinase and the actin filament network in angiotensin II-induced contraction and extracellular signal-regulated kinase activity in intact rat mesenteric resistance arteries. Arterioscler Thromb Vasc Biol. 21:1288–1293. 2001. View Article : Google Scholar

35 

Roberts RE: The role of Rho kinase and extracellular regulated kinase-mitogen-activated protein kinase in α2-adrenoceptor-mediaed vasoconstriction in the porcine palmar lateral vein. J Pharmacol Exp Ther. 311:742–747. 2004.

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Copy and paste a formatted citation
Spandidos Publications style
Zhang T, Shi WL, Tasker JG, Zhou JR, Peng YL, Miao CY, Yang YJ and Jiang CL: Dexamethasone induces rapid promotion of norepinephrine‑mediated vascular smooth muscle cell contraction. Mol Med Rep 7: 549-554, 2013.
APA
Zhang, T., Shi, W., Tasker, J.G., Zhou, J., Peng, Y., Miao, C. ... Jiang, C. (2013). Dexamethasone induces rapid promotion of norepinephrine‑mediated vascular smooth muscle cell contraction. Molecular Medicine Reports, 7, 549-554. https://doi.org/10.3892/mmr.2012.1196
MLA
Zhang, T., Shi, W., Tasker, J. G., Zhou, J., Peng, Y., Miao, C., Yang, Y., Jiang, C."Dexamethasone induces rapid promotion of norepinephrine‑mediated vascular smooth muscle cell contraction". Molecular Medicine Reports 7.2 (2013): 549-554.
Chicago
Zhang, T., Shi, W., Tasker, J. G., Zhou, J., Peng, Y., Miao, C., Yang, Y., Jiang, C."Dexamethasone induces rapid promotion of norepinephrine‑mediated vascular smooth muscle cell contraction". Molecular Medicine Reports 7, no. 2 (2013): 549-554. https://doi.org/10.3892/mmr.2012.1196
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang T, Shi WL, Tasker JG, Zhou JR, Peng YL, Miao CY, Yang YJ and Jiang CL: Dexamethasone induces rapid promotion of norepinephrine‑mediated vascular smooth muscle cell contraction. Mol Med Rep 7: 549-554, 2013.
APA
Zhang, T., Shi, W., Tasker, J.G., Zhou, J., Peng, Y., Miao, C. ... Jiang, C. (2013). Dexamethasone induces rapid promotion of norepinephrine‑mediated vascular smooth muscle cell contraction. Molecular Medicine Reports, 7, 549-554. https://doi.org/10.3892/mmr.2012.1196
MLA
Zhang, T., Shi, W., Tasker, J. G., Zhou, J., Peng, Y., Miao, C., Yang, Y., Jiang, C."Dexamethasone induces rapid promotion of norepinephrine‑mediated vascular smooth muscle cell contraction". Molecular Medicine Reports 7.2 (2013): 549-554.
Chicago
Zhang, T., Shi, W., Tasker, J. G., Zhou, J., Peng, Y., Miao, C., Yang, Y., Jiang, C."Dexamethasone induces rapid promotion of norepinephrine‑mediated vascular smooth muscle cell contraction". Molecular Medicine Reports 7, no. 2 (2013): 549-554. https://doi.org/10.3892/mmr.2012.1196
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