C/EBP homologous protein induces mesangial cell apoptosis under hyperglycemia

He,Daqiang; Li,Jianqiong; Zhao,Jiayao; Fei,Jing; Zhang,Xiaoming

doi:10.3892/mmr.2012.1234

C/EBP homologous protein induces mesangial cell apoptosis under hyperglycemia

Authors:
- Daqiang He
- Jianqiong Li
- Jiayao Zhao
- Jing Fei
- Xiaoming Zhang
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Affiliations: Department of Anatomy and Cell Biology, School of Medicine, Zhejiang University, Zhejiang 310058, P.R. China
Published online on: December 14, 2012 https://doi.org/10.3892/mmr.2012.1234
Pages: 445-448

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Abstract

Diabetes mellitus is known to cause kidney impairment; however, the mechanism remains elusive. The aim of this study was to investigate the role of C/EBP homologous protein (CHOP), an important protein in endoplasmic reticulum stress‑mediated mesangial cell apoptosis in hyperglycemia. Mesangial cells were cultured in normal (control group) and high glucose medium (high glucose group). TUNEL staining was performed to assess apoptotic cells in the groups. The expression of CHOP and caspase‑3 was also assayed by immunohistochemistry and western blot analysis. Following 24 h culture in high glucose medium, TUNEL‑positive cells were observed to be significantly increased (P<0.01). The expression of CHOP and caspase‑3 in mesangial cells was also found to be significantly enhanced under high glucose conditions compared with the normal group (P<0.01). The results indicate that CHOP mediates apoptosis in mesangial cells under hyperglycemia and may play a role in the development of diabetic nephropathy.

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