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Article

Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds

  • Authors:
    • Yanglin Gu
    • Peng Chen
    • Yusheng Yang
    • Keqin Shi
    • Yubin Wang
    • Wenhui Zhu
    • Guoxing Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Wuxi No. 2 People's Hospital, Jiangsu 214002, P.R. China, Department of Sports Medicine, Dongfang Hospital Affiliated to Tongji University, Shanghai 200120, P.R. China
  • Pages: 1003-1009
    |
    Published online on: December 18, 2012
       https://doi.org/10.3892/mmr.2012.1240
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Abstract

Myoblasts are considered to be an alternative cell source for cell-based meniscal repair due to their multiple differentiation potentials. This study addresses the chondrogenic differentiation of myoblasts seeded into poly-lactide-co-glycolide (PLGA) scaffolds following implantation in a subcutaneous pocket of nude mice. Canine myoblasts isolated from a Beagle were expanded and seeded into PLGA scaffolds and cultured in cartilage-derived morphogenetic protein-2 (CDMP-2) and transforming growth factor-β1 (TGF-β1)-containing medium for 2 weeks in vitro. The constructs were implanted into a subcutaneous pocket of 24 combined immunodeficiency mice and harvested after 8 and 12 weeks, respectively. Hematoxylin and eosin staining of the sections of the engineered cartilage at 8 and 12 weeks revealed the regeneration of fibrocartilage. Immunohistochemical staining confirmed a similar distribution of collagen type Ⅱ in the engineered cartilage as the normal meniscus. At 12 weeks, expression of mRNAs for type Ⅰ collagen, type Ⅱ collagen and aggrecan was detected by RT-PCR. The compressive moduli of engineered cartilage reached 85.72% of the normal meniscus at 12 weeks, with a high level of glycosaminoglycan (GAG) content (no statistical difference from normal). Myoblast-seeded PLGA scaffolds express a stable chondrogenic phenotype in a heterotopic model of cartilage transplantation and represent a suitable tool for tissue engineering of cartilage.
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Copy and paste a formatted citation
Spandidos Publications style
Gu Y, Chen P, Yang Y, Shi K, Wang Y, Zhu W and Zhu G: Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds. Mol Med Rep 7: 1003-1009, 2013.
APA
Gu, Y., Chen, P., Yang, Y., Shi, K., Wang, Y., Zhu, W., & Zhu, G. (2013). Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds. Molecular Medicine Reports, 7, 1003-1009. https://doi.org/10.3892/mmr.2012.1240
MLA
Gu, Y., Chen, P., Yang, Y., Shi, K., Wang, Y., Zhu, W., Zhu, G."Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds". Molecular Medicine Reports 7.3 (2013): 1003-1009.
Chicago
Gu, Y., Chen, P., Yang, Y., Shi, K., Wang, Y., Zhu, W., Zhu, G."Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds". Molecular Medicine Reports 7, no. 3 (2013): 1003-1009. https://doi.org/10.3892/mmr.2012.1240
Copy and paste a formatted citation
x
Spandidos Publications style
Gu Y, Chen P, Yang Y, Shi K, Wang Y, Zhu W and Zhu G: Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds. Mol Med Rep 7: 1003-1009, 2013.
APA
Gu, Y., Chen, P., Yang, Y., Shi, K., Wang, Y., Zhu, W., & Zhu, G. (2013). Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds. Molecular Medicine Reports, 7, 1003-1009. https://doi.org/10.3892/mmr.2012.1240
MLA
Gu, Y., Chen, P., Yang, Y., Shi, K., Wang, Y., Zhu, W., Zhu, G."Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds". Molecular Medicine Reports 7.3 (2013): 1003-1009.
Chicago
Gu, Y., Chen, P., Yang, Y., Shi, K., Wang, Y., Zhu, W., Zhu, G."Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds". Molecular Medicine Reports 7, no. 3 (2013): 1003-1009. https://doi.org/10.3892/mmr.2012.1240
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