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Article

The role of p21 3'UTR gene polymorphism in the risk of prostate cancer: A pilot study

  • Authors:
    • Monika Kmeťová Sivoňová
    • Marta Vilčková
    • Jana Jurečeková
    • Jozef Hatok
    • Dušan Dobrota
    • Róbert Dušenka
    • Ján Kliment
  • View Affiliations / Copyright

    Affiliations: Department of Medical Biochemistry, Comenius University in Bratislava, Jessenius Faculty of Medicine, Martin, Slovak Republic, Department of Urology, Comenius University in Bratislava, Jessenius Faculty of Medicine and University Hospital of Martin, Martin, Slovak Republic
  • Pages: 986-990
    |
    Published online on: December 18, 2012
       https://doi.org/10.3892/mmr.2012.1242
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Abstract

The cell cycle regulator p21 plays an important role in regulating critical cell activities including cell cycle control, DNA repair and apoptosis. Consequently, it may affect the efficacy of the response to DNA damage and tumor development. The aim of our study was to evaluate the frequencies of the p21 C70T polymorphism, the association between this genetic variant and smoking status, and the serum prostate-specific antigen (PSA) levels and Gleason score in 118 prostate cancer patients and 130 males routinely screened for prostate cancer in the Slovak population. Blood samples were collected from all individuals for DNA isolation, used for subsequent genotyping assays via PCR-RFLP methods. Overall, we did not observe any significant association between this polymorphism and prostate cancer risk. An analysis of the association between the p21 genotypes and smoking was then conducted. Among smokers, CC and CT genotypes were associated with a non‑significant increased risk (OR=1.48; 95% CI, 0.80-2.76 and OR=1.15; 95% CI, 0.27‑4.77, respectively; p>0.05) in comparison to non-smokers with the CC genotype. Patients with a CT genotype and serum PSA levels ≥10 ng/ml had an 84% decrease in prostate cancer risk (OR=0.16; 95% CI, 0.03-0.75; p<0.05) compared to cases with serum PSA levels <10 ng/ml and the CC genotype. No significant association was detected between Gleason score and prostate cancer risk. Based on these results, we concluded that the p21 C70T polymorphism is associated with decreased risk of prostate cancer in Slovak men. To confirm these findings, a systematic approach is required to identify sequence variants in this and other related genes, and subsequently, to test for an association between such variants, smoking status and tumor-specific clinicopathological characteristics in large samples.
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Copy and paste a formatted citation
Spandidos Publications style
Sivoňová MK, Vilčková M, Jurečeková J, Hatok J, Dobrota D, Dušenka R and Kliment J: The role of p21 3'UTR gene polymorphism in the risk of prostate cancer: A pilot study. Mol Med Rep 7: 986-990, 2013.
APA
Sivoňová, M.K., Vilčková, M., Jurečeková, J., Hatok, J., Dobrota, D., Dušenka, R., & Kliment, J. (2013). The role of p21 3'UTR gene polymorphism in the risk of prostate cancer: A pilot study. Molecular Medicine Reports, 7, 986-990. https://doi.org/10.3892/mmr.2012.1242
MLA
Sivoňová, M. K., Vilčková, M., Jurečeková, J., Hatok, J., Dobrota, D., Dušenka, R., Kliment, J."The role of p21 3'UTR gene polymorphism in the risk of prostate cancer: A pilot study". Molecular Medicine Reports 7.3 (2013): 986-990.
Chicago
Sivoňová, M. K., Vilčková, M., Jurečeková, J., Hatok, J., Dobrota, D., Dušenka, R., Kliment, J."The role of p21 3'UTR gene polymorphism in the risk of prostate cancer: A pilot study". Molecular Medicine Reports 7, no. 3 (2013): 986-990. https://doi.org/10.3892/mmr.2012.1242
Copy and paste a formatted citation
x
Spandidos Publications style
Sivoňová MK, Vilčková M, Jurečeková J, Hatok J, Dobrota D, Dušenka R and Kliment J: The role of p21 3'UTR gene polymorphism in the risk of prostate cancer: A pilot study. Mol Med Rep 7: 986-990, 2013.
APA
Sivoňová, M.K., Vilčková, M., Jurečeková, J., Hatok, J., Dobrota, D., Dušenka, R., & Kliment, J. (2013). The role of p21 3'UTR gene polymorphism in the risk of prostate cancer: A pilot study. Molecular Medicine Reports, 7, 986-990. https://doi.org/10.3892/mmr.2012.1242
MLA
Sivoňová, M. K., Vilčková, M., Jurečeková, J., Hatok, J., Dobrota, D., Dušenka, R., Kliment, J."The role of p21 3'UTR gene polymorphism in the risk of prostate cancer: A pilot study". Molecular Medicine Reports 7.3 (2013): 986-990.
Chicago
Sivoňová, M. K., Vilčková, M., Jurečeková, J., Hatok, J., Dobrota, D., Dušenka, R., Kliment, J."The role of p21 3'UTR gene polymorphism in the risk of prostate cancer: A pilot study". Molecular Medicine Reports 7, no. 3 (2013): 986-990. https://doi.org/10.3892/mmr.2012.1242
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