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Article

Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase

  • Authors:
    • Zhenran Wang
    • Wenjing Lu
    • Yang Li
    • Bo Tang
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, P.R. China, Department of Medical Oncology, Affiliated Hospital of Guilin Medical University, Guilin 541001, P.R. China, Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, P.R. China
  • Pages: 915-920
    |
    Published online on: December 18, 2012
       https://doi.org/10.3892/mmr.2012.1243
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Abstract

Alpinetin is a natural flavonoid widely distributed in Zingiberaceae. Previous studies have demonstrated that alpinetin markedly inhibits tumour growth. However, the molecular mechanisms underlying the antitumour effects of alpinetin are unclear. Bcl‑2‑associated X protein (Bax) translocation is known to activate the mitochondrial‑dependent endogenous apoptosis pathway. The aim of the current study was to investigate the roles of Bax and the mitochondrial pathway during alpinetin‑induced gastric cancer cell apoptosis and the effects of alpinetin on the cell cycle. Human gastric cancer cells were treated with various doses of alpinetin and an MTT assay was performed to measure cell viability, flow cytometry to measure the apoptotic rate, changes in the cell cycle and mitochondrial membrane potential and western blot analysis to detect the expression levels of relevant proteins. Results demonstrate that alpinetin induces apoptosis in human gastric cancer cells in a dose‑ and time‑dependent manner. During the early stages of apoptosis, alpinetin may alter mitochondrial membrane potential leading to release of cytochrome c from mitochondria, activation of caspase family members and ultimately apoptosis of human gastric cancer cells. Results of the present study indicate that alpinetin‑induced human gastric cancer cell apoptosis is associated with the mitochondrial pathway.
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1 

Kamangar F, Dores GM and Anderson WF: Patterns of cancer incidence, mortality and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world. J Clin Oncol. 24:2137–2150. 2006. View Article : Google Scholar

2 

Mackenzie M, Spithoff K and Jonker D: Systemic therapy for advanced gastric cancer: a clinical practice guideline. Curr Oncol. 18:e202–e209. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Wagner AD, Grothe W, Haerting J, Kleber G, Grothey A and Fleig WE: Chemotherapy in advanced gastric cancer: a systematic review and meta-analysis based on aggregate data. J Clin Oncol. 24:2903–2909. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Ngeow J, Tan IB and Choo SP: Targeted therapies in the treatment of gastric cancer. Asia Pac J Clin Oncol. 7:224–235. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Dikken JL, van de Velde CJ, Coit DG, Shah MA, Verheij M and Cats A: Treatment of resectable gastric cancer. Therap Adv Gastroenterol. 5:49–69. 2012. View Article : Google Scholar

6 

Takahashi H, Chen MC, Pham H, Angst E, King JC, Park J, Brovman EY, Ishiguro H, Harris DM, Reber HA, Hines OJ, Gukovskaya AS, Go VL and Eibl G: Baicalein, a component of Scutellaria baicalensis, induces apoptosis by Mcl-1 down-regulation in human pancreatic cancer cells. Biochim Biophys Acta. 1813:1465–1474. 2011.

7 

Gao J, Morgan WA, Sanchez-Medina A and Corcoran O: The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells. Toxicol Appl Pharmacol. 254:221–228. 2011.PubMed/NCBI

8 

Lee DH, Kim C, Zhang L and Lee YJ: Role of p53, PUMA and Bax in wogonin-induced apoptosis in human cancer cells. Biochem Pharmacol. 75:2020–2033. 2008. View Article : Google Scholar : PubMed/NCBI

9 

He W, Li Y, Tang J, Luan F, Jin J and Hu Z: Comparison of the characterization on binding of alpinetin and cardamonin to lysozyme by spectroscopic methods. Int J Biol Macromol. 39:165–173. 2006. View Article : Google Scholar : PubMed/NCBI

10 

In LL, Azmi MN, Ibrahim H, Awang K and Nagoor NH: 1′S-1′-acetoxyeugenol acetate: a novel phenylpropanoid from Alpinia conchigera enhances the apoptotic effects of paclitaxel in MCF-7 cells through NF-κB inactivation. Anticancer Drugs. 22:424–434. 2011.

11 

Malek SN, Phang CW, Ibrahim H, Norhanom AW and Sim KS: Phytochemical and cytotoxic investigations of Alpinia mutica rhizomes. Molecules. 16:583–589. 2011. View Article : Google Scholar : PubMed/NCBI

12 

He ZH, Ge W, Yue GG, Lau CB, He MF and But PP: Anti-angiogenic effects of the fruit of Alpinia oxyphylla. J Ethnopharmacol. 132:443–449. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Du J, Tang B, Wang J, Sui H, Jin X, Wang L and Wang Z: Antiproliferative effect of alpinetin in BxPC-3 pancreatic cancer cells. Int J Mol Med. 29:607–612. 2012.PubMed/NCBI

14 

Tang B, Zhang Y, Liang R, Yuan P, Du J, Wang H and Wang L: Activation of the δ-opioid receptor inhibits serum deprivation-induced apoptosis of human liver cells via the activation of PKC and the mitochondrial pathway. Int J Mol Med. 28:1077–1085. 2011.

15 

Hohenberger P and Gretschel S: Gastric cancer. Lancet. 362:305–315. 2003. View Article : Google Scholar

16 

Lee JH, Kim KM, Cheong JH and Noh SH: Current management and future strategies of gastric cancer. Yonsei Med J. 53:248–257. 2012. View Article : Google Scholar : PubMed/NCBI

17 

He W, Li Y, Xue C, Hu Z, Chen X and Sheng F: Effect of Chinese medicine alpinetin on the structure of human serum albumin. Bioorg Med Chem. 13:1837–1845. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Singh R, Singh S, Kumar S and Arora S: Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn. Food Chem Toxicol. 45:1216–1223. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Li F, Li C, Zhang H, Lu Z, Li Z, You Q, Lu N and Guo Q: VI-14, a novel flavonoid derivative, inhibits migration and invasion of human breast cancer cells. Toxicol Appl Pharmacol. 261:217–226. 2012. View Article : Google Scholar : PubMed/NCBI

20 

White JB, Beckford J, Yadegarynia S, Ngo N, Lialiutska T and d’Alarcao M: Some natural flavonoids are competitive inhibitors of Caspase-1, -3 and -7 despite their cellular toxicity. Food Chem. 131:1453–1459. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Salmela AL, Pouwels J, Kukkonen-Macchi A, Waris S, Toivonen P, Jaakkola K, Mäki-Jouppila J, Kallio L and Kallio MJ: The flavonoid eupatorin inactivates the mitotic checkpoint leading to polyploidy and apoptosis. Exp Cell Res. 318:578–592. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Wang B and Zhang X: Inhibitory effects of Broccolini leaf flavonoids on human cancer cells. Scanning. 34:1–5. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Konan NA, Lincopan N, Collantes Díaz IE, de Fátima Jacysyn J, Tanae Tiba MM, Amarante Mendes JG, Bacchi EM and Spira B: Cytotoxicity of cashew flavonoids towards malignant cell lines. Exp Toxicol Pathol. 64:435–440. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Tang J, Li N, Dai H and Wang K: Chemical constituents from seeds of Alpinia katsumadai, inhibition on NF-kappaB activation and anti-tumor effect. Zhongguo Zhong Yao Za Zhi. 35:1710–1714. 2010.(In Chinese).

25 

Evan G and Littlewood T: A matter of life and cell death. Science. 281:1317–1322. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Williams GT: Programmed cell death: apoptosis and oncogenesis. Cell. 65:1097–1098. 1991. View Article : Google Scholar : PubMed/NCBI

27 

Powathil GG, Gordon KE, Hill LA and Chaplain MA: Modelling the effects of cell-cycle heterogeneity on the response of a solid tumour to chemotherapy: Biological insights from a hybrid multiscale cellular automaton model. J Theor Biol. 308:1–19. 2012. View Article : Google Scholar

28 

Wang XM, Cui JW, Li W, Cai L, Song W and Wang GJ: Silencing of the COPS3 gene by siRNA reduces proliferation of lung cancer cells most likely via induction of cell cycle arrest and apoptosis. Asian Pac J Cancer Prev. 13:1043–1048. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Pietrantonio F, Biondani P, de Braud F, Pellegrinelli A, Bianchini G, Perrone F, Formisano B and Di Bartolomeo M: Bax expression is predictive of favorable clinical outcome in chemonaive advanced gastric cancer patients treated with capecitabine, oxaliplatin and irinotecan regimen. Transl Oncol. 5:155–159. 2012. View Article : Google Scholar

30 

Burlacu A: Regulation of apoptosis by Bcl-2 family proteins. J Cell Mol Med. 7:249–257. 2003. View Article : Google Scholar : PubMed/NCBI

31 

Szabò I, Soddemann M, Leanza L, Zoratti M and Gulbins E: Single-point mutations of a lysine residue change function of Bax and Bcl-xL expressed in Bax- and Bak-less mouse embryonic fibroblasts: novel insights into the molecular mechanisms of Bax-induced apoptosis. Cell Death Differ. 18:427–438. 2011.

32 

Zeng L, Li T, Xu DC, Liu J, Mao G, Cui MZ, Fu X and Xu X: Death receptor 6 induces apoptosis not through type I or type II pathways, but via a unique mitochondria-dependent pathway by interacting with bax protein. J Biol Chem. 287:29125–29133. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Riedl SJ and Shi Y: Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol. 5:897–907. 2004. View Article : Google Scholar : PubMed/NCBI

34 

Eldering E, Mackus WJ, Derks IA, Evers LM, Beuling E, Teeling P, Lens SM, van Oers MH and van Lier RA: Apoptosis via the B cell antigen receptor requires Bax translocation and involves mitochondrial depolarization, cytochrome C release and caspase-9 activation. Eur J Immunol. 34:1950–1960. 2004. View Article : Google Scholar

35 

Chen M and Wang J: Initiator caspases in apoptosis signaling pathways. Apoptosis. 7:313–319. 2002. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Lu W, Li Y and Tang B: Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase. Mol Med Rep 7: 915-920, 2013.
APA
Wang, Z., Lu, W., Li, Y., & Tang, B. (2013). Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase. Molecular Medicine Reports, 7, 915-920. https://doi.org/10.3892/mmr.2012.1243
MLA
Wang, Z., Lu, W., Li, Y., Tang, B."Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase". Molecular Medicine Reports 7.3 (2013): 915-920.
Chicago
Wang, Z., Lu, W., Li, Y., Tang, B."Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase". Molecular Medicine Reports 7, no. 3 (2013): 915-920. https://doi.org/10.3892/mmr.2012.1243
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Lu W, Li Y and Tang B: Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase. Mol Med Rep 7: 915-920, 2013.
APA
Wang, Z., Lu, W., Li, Y., & Tang, B. (2013). Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase. Molecular Medicine Reports, 7, 915-920. https://doi.org/10.3892/mmr.2012.1243
MLA
Wang, Z., Lu, W., Li, Y., Tang, B."Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase". Molecular Medicine Reports 7.3 (2013): 915-920.
Chicago
Wang, Z., Lu, W., Li, Y., Tang, B."Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase". Molecular Medicine Reports 7, no. 3 (2013): 915-920. https://doi.org/10.3892/mmr.2012.1243
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