Downregulation of Id1 by small interfering RNA in gastric cancer inhibits cell growth via the Akt pathway

Yang,Guang; Zhang,Yan; Xiong,Jianjun; Wu,Jing; Yang,Changfu; Huang,Hongbing; Zhu,Zhenyu

doi:10.3892/mmr.2012.749

Downregulation of Id1 by small interfering RNA in gastric cancer inhibits cell growth via the Akt pathway

Authors:
- Guang Yang
- Yan Zhang
- Jianjun Xiong
- Jing Wu
- Changfu Yang
- Hongbing Huang
- Zhenyu Zhu
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Affiliations: Department of Biochemistry and Molecular Biology, Zhongshan Medical College, Sun Yat-Sen University, Guangdong 510080, P.R. China, The Center of Prenatal Diagnosis, Maternal and Child Health Hospital of Guangdong Province, Guangdong, P.R. China, Department of Pharmacy, State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangdong 510080, P.R. China
Published online on: January 12, 2012 https://doi.org/10.3892/mmr.2012.749
Pages: 1075-1079

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Abstract

Inhibitor of differentiation or DNA binding (Id1) is a member of the helix-loop-helix transcription factor family that is overexpressed in various types of cancer, including gastric carcinoma. Previous studies showed that Id1 is a prognostic marker in patients with gastric cancer. However, the role of Id1 in the proliferation of human gastric cancer cells has yet to be clarified. In the present study, we downregulated the Id1 gene in SGC-7901 gastric cancer cells by RNA interference, and we also constructed a recombinant plasmid-expressing Id1 to investigate its effects on the proliferation of SGC-7901 cells. Results showed that the downregulation of Id1 inhibited proliferation of SGC-7901 cells, while the upregulation of Id1 had no effect on SGC-7901 cell proliferation. The potential mechanism was also investigated. The changes of certain proteins associated with cell proliferation, apoptosis and the cell cycle were detected by western blotting. Furthermore, we demonstrated a positive correlation between Id1 and phospho-Akt expression in SGC-7901 cells.

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