Correlation between hammerhead ribozyme-mediated eggshell protein gene cleavage and reproduction inhibition of Schistosoma japonicum

Liang,Yu; Zhou,Yuelan; Yin,Weiguo; Li,Yingju; Yang,Qiulin; Gao,Yuan; Zhang,Yukuai; Yang,Yaofei; Peng,Li; Xiao,Jianhua

doi:10.3892/mmr.2012.752

April 2012 Volume 5 Issue 4

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April 2012 Volume 5 Issue 4

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Article

Correlation between hammerhead ribozyme-mediated eggshell protein gene cleavage and reproduction inhibition of Schistosoma japonicum

Authors:
- Yu Liang
- Yuelan Zhou
- Weiguo Yin
- Yingju Li
- Qiulin Yang
- Yuan Gao
- Yukuai Zhang
- Yaofei Yang
- Li Peng
- Jianhua Xiao
View Affiliations / Copyright

Affiliations: Institute of Pathogenic Biology, University of South China, Hengyang, Hunan 421001, P.R. China, The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China, Institute of Pathogenic Biology, University of South China, Hengyang, Hunan 421001, P.R. China
Pages: 1087-1091
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Published online on: January 12, 2012

https://doi.org/10.3892/mmr.2012.752
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Abstract

Schistosoma japonicum (S. japonicum) is an extremely harmful pathogen, which infects humans and causes severe public health problems. To date, no effective therapeutic drugs for this pathogen are available. In this study, we designed and constructed three hammerhead ribozymes targeting the eggshell protein gene of S. japonicum (SjESG). The cleavage activities of these three ribozymes were determined using cleavage experiments. The in vitro cleavage results showed that among the three synthesized ribozymes (Rz1, Rz2 and Rz3), Rz1 and Rz3 cleaved their target RNAs effectively. However, Rz2 did not cleave its target RNA detectably. The putative therapeutic roles of these three ribozymes to inhibit the reproduction of S. japonicum in mice were studied in vivo. Compared with the negative controls, Rz1 and Rz3 treatments resulted in increased levels of IFN-γ but decreased levels of IL-4 in mice. Rz2 affected levels of IFN-γ and IL-4 to degrees similar with those caused by the vector controls. In addition, Rz1 and Rz3 reduced the amounts of adult worms and eggs in the livers of mice more extensively than Rz2 and the vector controls. Altogether, these results suggest a correlation between the in vitro cleavage abilities of Rz1 and Rz3 and their roles in reproduction inhibition of S. japonicum.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Correlation between hammerhead ribozyme-mediated eggshell protein gene cleavage and reproduction inhibition of Schistosoma japonicum

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