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Molecular Medicine Reports
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April 2012 Volume 5 Issue 4

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International Journal of Molecular Medicine

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Article

Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells

  • Authors:
    • Xiang-Qi Chen
    • Sheng Yang
    • Zhi-Ying Li
    • Hui-Shan Lu
    • Ming-Qiang Kang
    • Ting-Yan Lin
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The Union Hospital of FuJian Medical University, Fuzhou 350001, P.R. China, Department of Oncology, The Union Hospital of FuJian Medical University, Fuzhou 350001, P.R. China, Department of Thoracic Surgery, The Union Hospital of FuJian Medical University, Fuzhou 350001, P.R. China
  • Pages: 917-922
    |
    Published online on: January 13, 2012
       https://doi.org/10.3892/mmr.2012.755
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Abstract

The survivin protein, a member of the inhibitors of apoptosis (IAP) family, has gained popularity as a therapeutic target for cancer due to its selective expression in tumor cells and its significant involvement in tumor cell viability. The aim of this study was to investigate the effect of the survivin-small interfering RNA (siRNA) plasmid on survivin expression in the human lung cancer cell line, A549, and to observe its effects on apoptosis and proliferation of A549 cells. A549 human lung cancer cells were transfected with survivin-targeting siRNA. The downregulation of survivin expression was determined by real-time polymerase chain reaction and western blotting. The proliferation of A549 cells was determined by MTT assay. The apoptotic rate and cell cycle distribution were analyzed by flow cytometry (FCM). Caspase-9 activity was also detected to study the apoptosis of lung cancer cells induced by siRNA against survivin. The sequence-specific siRNA efficiently and specifically downregulated the expression of survivin at both the mRNA and protein levels. Downregulation of survivin expression dramatically suppressed the proliferation of A549 cells and arrested the cells at the G (1)/G (0) phase. Caspase-9 activity was significantly increased in A549 cells transfected with siRNA against survivin. In this study, we found that survivin-specific siRNA can efficiently suppress the expression of survivin, increase apoptosis and inhibit A549 cell proliferation. Our findings further indicate the possibility that the antitumor effects of survivin-siRNA are mediated through the activation of caspase-9.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Yang S, Li Z, Lu H, Kang M and Lin T: Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells. Mol Med Rep 5: 917-922, 2012.
APA
Chen, X., Yang, S., Li, Z., Lu, H., Kang, M., & Lin, T. (2012). Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells. Molecular Medicine Reports, 5, 917-922. https://doi.org/10.3892/mmr.2012.755
MLA
Chen, X., Yang, S., Li, Z., Lu, H., Kang, M., Lin, T."Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells". Molecular Medicine Reports 5.4 (2012): 917-922.
Chicago
Chen, X., Yang, S., Li, Z., Lu, H., Kang, M., Lin, T."Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells". Molecular Medicine Reports 5, no. 4 (2012): 917-922. https://doi.org/10.3892/mmr.2012.755
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Yang S, Li Z, Lu H, Kang M and Lin T: Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells. Mol Med Rep 5: 917-922, 2012.
APA
Chen, X., Yang, S., Li, Z., Lu, H., Kang, M., & Lin, T. (2012). Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells. Molecular Medicine Reports, 5, 917-922. https://doi.org/10.3892/mmr.2012.755
MLA
Chen, X., Yang, S., Li, Z., Lu, H., Kang, M., Lin, T."Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells". Molecular Medicine Reports 5.4 (2012): 917-922.
Chicago
Chen, X., Yang, S., Li, Z., Lu, H., Kang, M., Lin, T."Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells". Molecular Medicine Reports 5, no. 4 (2012): 917-922. https://doi.org/10.3892/mmr.2012.755
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