Effect of radiation on the expression of osteoclast marker genes in RAW264.7 cells

Yang,Bing; Zhou,Hui; Zhang,Xiao-Dong; Liu,Zheng; Fan,Fei-Yue; Sun,Yuan-Ming

doi:10.3892/mmr.2012.765

April 2012 Volume 5 Issue 4

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April 2012 Volume 5 Issue 4

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Article

Effect of radiation on the expression of osteoclast marker genes in RAW264.7 cells

Authors:
- Bing Yang
- Hui Zhou
- Xiao-Dong Zhang
- Zheng Liu
- Fei-Yue Fan
- Yuan-Ming Sun
View Affiliations / Copyright

Affiliations: Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, P.R. China, School of Pharmacy, Tianjin Medical University, Tianjin 300070, P.R. China
Pages: 955-958
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Published online on: January 25, 2012

https://doi.org/10.3892/mmr.2012.765
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Abstract

Cancer radiation therapy can cause skeletal complications, such as osteopenia and osteoporosis. To understand the mechanism responsible for the skeletal complications, the expression profiles of osteoclast marker genes in RAW264.7 cells were observed. Osteoclast formation was established by RAW264.7 cells that were treated with the receptor activator of nuclear factor (NF)-κB ligand (RANKL) and detected using immunochemistry and morphological observations. Quantitative real-time polymerase chain reaction was used to assess the expression of a panel of osteoclast markers, including the receptor activator of NF-κB (RANK), tartrate-resistant acid phosphatase (TRAP), integrin β3 and the calcitonin receptor (CTR). RANKL-induced osteoclasts were TRAP-positive and multinucleated, and displayed a distinct morphology. RANKL-induced osteoclast precursor cells had increased TRAP and RANK expression and decreased CTR expression compared to the control cells not treated with RANKL. RAW264.7 cells irradiated with 2-Gy γ-rays had upregulated integrin β3 and RANK expression and downregulated CTR expression compared to the control RAW264.7 cells. The effect of radiation on RANKL-induced osteoclast differentiation enhanced the expression of CTR and inhibited the expression of RANK and TRAP. Therefore, radiation damage from 2-Gy γ-rays can promote the activities of osteoclast precursor cells, but not those of osteoclasts.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of radiation on the expression of osteoclast marker genes in RAW264.7 cells

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