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Article

Silencing of the polyamine catabolic key enzyme SSAT prevents CDK inhibitor-induced apoptosis in Caco-2 colon cancer cells

  • Authors:
    • A. Çoker
    • E. D. Arısan
    • N. Palavan-Ünsal
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology and Genetics, Science and Literature Faculty, Istanbul Kultur University, Atakoy Campus, 34156 Istanbul, Turkey
  • Pages: 1037-1042
    |
    Published online on: January 30, 2012
       https://doi.org/10.3892/mmr.2012.768
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Abstract

Roscovitine and purvalanol are purine derivative cyclin-dependent kinase (CDK) inhibitors that induce apoptosis in various types of cancer cells. However, their impact on the apoptotic cell death mechanism requires further elucidation. Natural polyamines putrescine, spermidine and spermine play essential roles in the regulation of cell growth and proliferation. Increased levels of polyamines in cells are considered to be involved in cancer progression. Intracellular polyamine levels are under the control of several catabolic enzymes, such as spermidine/spermine-N-acetyl transferase (SSAT), acetylpolyamine oxidase (APAO) and spermine oxidase (SMO), which could be altered by several therapeutic drugs. However, the possible role of polyamines in drug-induced apoptosis has yet to be clarified. In the present study, our aim was to determine the modulation of the polyamine catabolic pathway related to CDK inhibitor-induced apoptosis in Caco-2 cells. We found that roscovitine and purvalanol (each 20 µM) induced apoptosis by activating caspase-9 and -3, and inhibiting the mitochondrial membrane potential in Caco-2 cells. CDK inhibitors decreased the intracellular putrescine and spermine levels without affecting spermidine levels. Although both roscovitine and purvalanol induced SSAT expression, they did not exert a significant effect on the APAO expression profile. SSAT transient silencing prevented roscovitine-induced apoptosis compared to parental cells. Thus, we concluded that roscovitine and purvalanol significantly induce apoptosis in Caco-2 cells by modulating the polyamine catabolism, and that SSAT could be an important target in evaluating the potential role of polyamines in apoptotic cell death.
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Copy and paste a formatted citation
Spandidos Publications style
Çoker A, Arısan ED and Palavan-Ünsal N: Silencing of the polyamine catabolic key enzyme SSAT prevents CDK inhibitor-induced apoptosis in Caco-2 colon cancer cells. Mol Med Rep 5: 1037-1042, 2012.
APA
Çoker, A., Arısan, E.D., & Palavan-Ünsal, N. (2012). Silencing of the polyamine catabolic key enzyme SSAT prevents CDK inhibitor-induced apoptosis in Caco-2 colon cancer cells. Molecular Medicine Reports, 5, 1037-1042. https://doi.org/10.3892/mmr.2012.768
MLA
Çoker, A., Arısan, E. D., Palavan-Ünsal, N."Silencing of the polyamine catabolic key enzyme SSAT prevents CDK inhibitor-induced apoptosis in Caco-2 colon cancer cells". Molecular Medicine Reports 5.4 (2012): 1037-1042.
Chicago
Çoker, A., Arısan, E. D., Palavan-Ünsal, N."Silencing of the polyamine catabolic key enzyme SSAT prevents CDK inhibitor-induced apoptosis in Caco-2 colon cancer cells". Molecular Medicine Reports 5, no. 4 (2012): 1037-1042. https://doi.org/10.3892/mmr.2012.768
Copy and paste a formatted citation
x
Spandidos Publications style
Çoker A, Arısan ED and Palavan-Ünsal N: Silencing of the polyamine catabolic key enzyme SSAT prevents CDK inhibitor-induced apoptosis in Caco-2 colon cancer cells. Mol Med Rep 5: 1037-1042, 2012.
APA
Çoker, A., Arısan, E.D., & Palavan-Ünsal, N. (2012). Silencing of the polyamine catabolic key enzyme SSAT prevents CDK inhibitor-induced apoptosis in Caco-2 colon cancer cells. Molecular Medicine Reports, 5, 1037-1042. https://doi.org/10.3892/mmr.2012.768
MLA
Çoker, A., Arısan, E. D., Palavan-Ünsal, N."Silencing of the polyamine catabolic key enzyme SSAT prevents CDK inhibitor-induced apoptosis in Caco-2 colon cancer cells". Molecular Medicine Reports 5.4 (2012): 1037-1042.
Chicago
Çoker, A., Arısan, E. D., Palavan-Ünsal, N."Silencing of the polyamine catabolic key enzyme SSAT prevents CDK inhibitor-induced apoptosis in Caco-2 colon cancer cells". Molecular Medicine Reports 5, no. 4 (2012): 1037-1042. https://doi.org/10.3892/mmr.2012.768
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