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Article

γ-tocotrienol attenuates TNF-α-induced changes in secretion and gene expression of MCP-1, IL-6 and adiponectin in 3T3-L1 adipocytes

  • Authors:
    • Tetsuro Matsunaga
    • Ayae Shoji
    • Ning Gu
    • Erina Joo
    • Shiho Li
    • Tetsuya Adachi
    • Hanae Yamazaki
    • Koichiro Yasuda
    • Takashi Kondoh
    • Kinsuke Tsuda
  • View Affiliations / Copyright

    Affiliations: Ajinomoto Integrative Research for Advanced Dieting, Graduate School of Agriculture, Kyoto University, Kyoto, Japan, Laboratory of Metabolism, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan, Department of Life Science and Engineering, Harbin Institute of Technology, Harbin, P.R. China, Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine, Kyoto, Japan, Saiseikai-Noe Hospital, Osaka, Japan
  • Pages: 905-909
    |
    Published online on: January 30, 2012
       https://doi.org/10.3892/mmr.2012.770
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Abstract

Tocotrienols, members of the vitamin E family, have been shown to possess anti-inflammatory properties and display activity against a variety of chronic diseases, such as cancer, cardiovascular and neurological diseases. However, whether tocotrienols contribute to the prevention of inflammatory responses in adipose tissue remains to be elucidated. In this study, we examined the effects of γ-tocotrienol, the most common tocotrienol isomer, on tumor necrosis factor-α (TNF-α)-induced inflammatory responses by measuring the expression of the adipokines, monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) and adiponectin in 3T3-L1 adipocytes. Exposure to TNF-α (10 ng/ml) for 24 h increased MCP-1 and IL-6 secretion, and decreased adiponectin secretion and peroxisome proliferator-activated receptor-γ (PPARγ) mRNA expression. γ-tocotrienol effectively improved the TNF-α-induced adverse changes in MCP-1, IL-6 and adiponectin secretion, and in MCP-1, IL-6, adiponectin and PPARγ mRNA expression. Furthermore, TNF-α-mediated IκB-α phosphorylation and nuclear factor-κB (NF-κB) activation were significantly suppressed by the γ-tocotrienol treatment. Our results suggest that γ-tocotrienol may improve obesity-related functional abnormalities in adipocytes by attenuating NF-κB activation and the expression of inflammatory adipokines.
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Copy and paste a formatted citation
Spandidos Publications style
Matsunaga T, Shoji A, Gu N, Joo E, Li S, Adachi T, Yamazaki H, Yasuda K, Kondoh T, Tsuda K, Tsuda K, et al: γ-tocotrienol attenuates TNF-α-induced changes in secretion and gene expression of MCP-1, IL-6 and adiponectin in 3T3-L1 adipocytes. Mol Med Rep 5: 905-909, 2012.
APA
Matsunaga, T., Shoji, A., Gu, N., Joo, E., Li, S., Adachi, T. ... Tsuda, K. (2012). γ-tocotrienol attenuates TNF-α-induced changes in secretion and gene expression of MCP-1, IL-6 and adiponectin in 3T3-L1 adipocytes. Molecular Medicine Reports, 5, 905-909. https://doi.org/10.3892/mmr.2012.770
MLA
Matsunaga, T., Shoji, A., Gu, N., Joo, E., Li, S., Adachi, T., Yamazaki, H., Yasuda, K., Kondoh, T., Tsuda, K."γ-tocotrienol attenuates TNF-α-induced changes in secretion and gene expression of MCP-1, IL-6 and adiponectin in 3T3-L1 adipocytes". Molecular Medicine Reports 5.4 (2012): 905-909.
Chicago
Matsunaga, T., Shoji, A., Gu, N., Joo, E., Li, S., Adachi, T., Yamazaki, H., Yasuda, K., Kondoh, T., Tsuda, K."γ-tocotrienol attenuates TNF-α-induced changes in secretion and gene expression of MCP-1, IL-6 and adiponectin in 3T3-L1 adipocytes". Molecular Medicine Reports 5, no. 4 (2012): 905-909. https://doi.org/10.3892/mmr.2012.770
Copy and paste a formatted citation
x
Spandidos Publications style
Matsunaga T, Shoji A, Gu N, Joo E, Li S, Adachi T, Yamazaki H, Yasuda K, Kondoh T, Tsuda K, Tsuda K, et al: γ-tocotrienol attenuates TNF-α-induced changes in secretion and gene expression of MCP-1, IL-6 and adiponectin in 3T3-L1 adipocytes. Mol Med Rep 5: 905-909, 2012.
APA
Matsunaga, T., Shoji, A., Gu, N., Joo, E., Li, S., Adachi, T. ... Tsuda, K. (2012). γ-tocotrienol attenuates TNF-α-induced changes in secretion and gene expression of MCP-1, IL-6 and adiponectin in 3T3-L1 adipocytes. Molecular Medicine Reports, 5, 905-909. https://doi.org/10.3892/mmr.2012.770
MLA
Matsunaga, T., Shoji, A., Gu, N., Joo, E., Li, S., Adachi, T., Yamazaki, H., Yasuda, K., Kondoh, T., Tsuda, K."γ-tocotrienol attenuates TNF-α-induced changes in secretion and gene expression of MCP-1, IL-6 and adiponectin in 3T3-L1 adipocytes". Molecular Medicine Reports 5.4 (2012): 905-909.
Chicago
Matsunaga, T., Shoji, A., Gu, N., Joo, E., Li, S., Adachi, T., Yamazaki, H., Yasuda, K., Kondoh, T., Tsuda, K."γ-tocotrienol attenuates TNF-α-induced changes in secretion and gene expression of MCP-1, IL-6 and adiponectin in 3T3-L1 adipocytes". Molecular Medicine Reports 5, no. 4 (2012): 905-909. https://doi.org/10.3892/mmr.2012.770
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