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August 2012 Volume 6 Issue 2

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Article

Identification of a new protein biomarker for colorectal cancer diagnosis

  • Authors:
    • Xiao-Hui Zhai
    • Jie-Kai Yu
    • Fu-Quan Yang
    • Shu Zheng
  • View Affiliations / Copyright

    Affiliations: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, P.R. China, Proteomic Platform, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P.R. China
  • Pages: 444-448
    |
    Published online on: May 21, 2012
       https://doi.org/10.3892/mmr.2012.923
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Abstract

As one of the most common cancers, colorectal cancer (CRC) is a major public health issue worldwide. Thus, the identification of novel biomarkers to aid in the early diagnosis of CRC is crucial. The aim of the present study was to identify a novel protein biomarker for CRC, and to identify its structure. In this study, a total of 99 serum samples from 73 CRC patients and 26 healthy controls were collected and analyzed by SELDI-TOF-MS. The biomarkers were separated using HPLC and detected with MALDI-TOF-MS. The qualified peaks were ranked by p-value of non-parametric tests and the top 10 peaks displaying significant differences were selected. Among the 10 protein biomarkers, the concentration of a 3.9‑kDa protein in the serum of the CRC patients was much lower than that in the healthy controls. Therefore, the 3.9‑kDa protein was selected as a biomarker for CRC and its separation and purification were performed. The structure of the 3.9-kDa protein biomarker was determined by LC-MS/MS, and was confirmed to be a fragment of serine/theonine kinase 4 (MST1/STK4). The 3.9‑kDa protein biomarker had high sensitivity and specificity for CRC, and its potential clinical application warrants further investigation.
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Copy and paste a formatted citation
Spandidos Publications style
Zhai X, Yu J, Yang F and Zheng S: Identification of a new protein biomarker for colorectal cancer diagnosis. Mol Med Rep 6: 444-448, 2012.
APA
Zhai, X., Yu, J., Yang, F., & Zheng, S. (2012). Identification of a new protein biomarker for colorectal cancer diagnosis. Molecular Medicine Reports, 6, 444-448. https://doi.org/10.3892/mmr.2012.923
MLA
Zhai, X., Yu, J., Yang, F., Zheng, S."Identification of a new protein biomarker for colorectal cancer diagnosis". Molecular Medicine Reports 6.2 (2012): 444-448.
Chicago
Zhai, X., Yu, J., Yang, F., Zheng, S."Identification of a new protein biomarker for colorectal cancer diagnosis". Molecular Medicine Reports 6, no. 2 (2012): 444-448. https://doi.org/10.3892/mmr.2012.923
Copy and paste a formatted citation
x
Spandidos Publications style
Zhai X, Yu J, Yang F and Zheng S: Identification of a new protein biomarker for colorectal cancer diagnosis. Mol Med Rep 6: 444-448, 2012.
APA
Zhai, X., Yu, J., Yang, F., & Zheng, S. (2012). Identification of a new protein biomarker for colorectal cancer diagnosis. Molecular Medicine Reports, 6, 444-448. https://doi.org/10.3892/mmr.2012.923
MLA
Zhai, X., Yu, J., Yang, F., Zheng, S."Identification of a new protein biomarker for colorectal cancer diagnosis". Molecular Medicine Reports 6.2 (2012): 444-448.
Chicago
Zhai, X., Yu, J., Yang, F., Zheng, S."Identification of a new protein biomarker for colorectal cancer diagnosis". Molecular Medicine Reports 6, no. 2 (2012): 444-448. https://doi.org/10.3892/mmr.2012.923
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