Identification of a new protein biomarker for colorectal cancer diagnosis

  • Authors:
    • Xiao-Hui Zhai
    • Jie-Kai Yu
    • Fu-Quan Yang
    • Shu Zheng
  • View Affiliations

  • Published online on: May 21, 2012     https://doi.org/10.3892/mmr.2012.923
  • Pages: 444-448
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Abstract

As one of the most common cancers, colorectal cancer (CRC) is a major public health issue worldwide. Thus, the identification of novel biomarkers to aid in the early diagnosis of CRC is crucial. The aim of the present study was to identify a novel protein biomarker for CRC, and to identify its structure. In this study, a total of 99 serum samples from 73 CRC patients and 26 healthy controls were collected and analyzed by SELDI-TOF-MS. The biomarkers were separated using HPLC and detected with MALDI-TOF-MS. The qualified peaks were ranked by p-value of non-parametric tests and the top 10 peaks displaying significant differences were selected. Among the 10 protein biomarkers, the concentration of a 3.9‑kDa protein in the serum of the CRC patients was much lower than that in the healthy controls. Therefore, the 3.9‑kDa protein was selected as a biomarker for CRC and its separation and purification were performed. The structure of the 3.9-kDa protein biomarker was determined by LC-MS/MS, and was confirmed to be a fragment of serine/theonine kinase 4 (MST1/STK4). The 3.9‑kDa protein biomarker had high sensitivity and specificity for CRC, and its potential clinical application warrants further investigation.
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August 2012
Volume 6 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhai X, Yu J, Yang F and Zheng S: Identification of a new protein biomarker for colorectal cancer diagnosis. Mol Med Rep 6: 444-448, 2012
APA
Zhai, X., Yu, J., Yang, F., & Zheng, S. (2012). Identification of a new protein biomarker for colorectal cancer diagnosis. Molecular Medicine Reports, 6, 444-448. https://doi.org/10.3892/mmr.2012.923
MLA
Zhai, X., Yu, J., Yang, F., Zheng, S."Identification of a new protein biomarker for colorectal cancer diagnosis". Molecular Medicine Reports 6.2 (2012): 444-448.
Chicago
Zhai, X., Yu, J., Yang, F., Zheng, S."Identification of a new protein biomarker for colorectal cancer diagnosis". Molecular Medicine Reports 6, no. 2 (2012): 444-448. https://doi.org/10.3892/mmr.2012.923