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Article

Effects of curcumin on bleomycin‑induced oxidative stress in malignant testicular germ cell tumors

  • Authors:
    • Aysegul Cort
    • Evrim Ozdemir
    • Mujgan Timur
    • Tomris Ozben
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Faculty of Medicine, Akdeniz University, Antalya 07070, Turkey
  • Pages: 860-866
    |
    Published online on: July 17, 2012
       https://doi.org/10.3892/mmr.2012.991
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Abstract

Bleomycin is commonly used in the treatment of testicular cancer. Bleomycin generates oxygen radicals, induces the oxidative cleavage of DNA strands and induces cancer cell apoptosis. Curcumin (diferuloylmethane) is a potent antioxidant and chief component of the spice turmeric. No study investigating the effects of curcumin on intrinsic and bleomycin-induced oxidative stress in testicular germ cell tumors has been reported in the literature. For this reason, the present study aimed to examine the effects of curcumin on oxidative stress produced in wild-type NTera-2 and p53-mutant NCCIT testicular cancer cells incubated with bleomycin and the results were compared with cells treated with H2O2 which directly produces oxidative stress. The protein carbonyl content, thiobarbituric acid reactive substances (TBARS), glutathione (GSH), 8-isoprostane, lipid hydroperoxide (LPO) levels and total antioxidant capacity in the two testicular cancer cell lines were determined. Results showed that bleomycin and H2O2 significantly increased protein carbonyl, TBARS, 8-isoprostane and LPO levels in the NTera-2 and NCCIT cell lines. Bleomycin and H2O2 significantly decreased the antioxidant capacity and GSH levels in NTera-2 cells. Curcumin significantly decreased LPO, 8-isoprostane and protein carbonyl content, and TBARS levels increased in cells treated with bleomycin and H2O2. Curcumin enhanced GSH levels and the antioxidant capacity of NTera-2 cells. In conclusion, curcumin inhibits bleomycin and H2O2-induced oxidative stress in human testicular cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Cort A, Ozdemir E, Timur M and Ozben T: Effects of curcumin on bleomycin‑induced oxidative stress in malignant testicular germ cell tumors. Mol Med Rep 6: 860-866, 2012.
APA
Cort, A., Ozdemir, E., Timur, M., & Ozben, T. (2012). Effects of curcumin on bleomycin‑induced oxidative stress in malignant testicular germ cell tumors. Molecular Medicine Reports, 6, 860-866. https://doi.org/10.3892/mmr.2012.991
MLA
Cort, A., Ozdemir, E., Timur, M., Ozben, T."Effects of curcumin on bleomycin‑induced oxidative stress in malignant testicular germ cell tumors". Molecular Medicine Reports 6.4 (2012): 860-866.
Chicago
Cort, A., Ozdemir, E., Timur, M., Ozben, T."Effects of curcumin on bleomycin‑induced oxidative stress in malignant testicular germ cell tumors". Molecular Medicine Reports 6, no. 4 (2012): 860-866. https://doi.org/10.3892/mmr.2012.991
Copy and paste a formatted citation
x
Spandidos Publications style
Cort A, Ozdemir E, Timur M and Ozben T: Effects of curcumin on bleomycin‑induced oxidative stress in malignant testicular germ cell tumors. Mol Med Rep 6: 860-866, 2012.
APA
Cort, A., Ozdemir, E., Timur, M., & Ozben, T. (2012). Effects of curcumin on bleomycin‑induced oxidative stress in malignant testicular germ cell tumors. Molecular Medicine Reports, 6, 860-866. https://doi.org/10.3892/mmr.2012.991
MLA
Cort, A., Ozdemir, E., Timur, M., Ozben, T."Effects of curcumin on bleomycin‑induced oxidative stress in malignant testicular germ cell tumors". Molecular Medicine Reports 6.4 (2012): 860-866.
Chicago
Cort, A., Ozdemir, E., Timur, M., Ozben, T."Effects of curcumin on bleomycin‑induced oxidative stress in malignant testicular germ cell tumors". Molecular Medicine Reports 6, no. 4 (2012): 860-866. https://doi.org/10.3892/mmr.2012.991
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