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Article

Inhibitory effect of nicardipine on rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells

  • Authors:
    • Hae Jeong Park
    • Hak‑Jae Kim
  • View Affiliations / Copyright

    Affiliations: Kohwang Medical Research Institute, School of Medicine, Kyung Hee University, Seoul, Republic of Korea, Department of Microbiology, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea
  • Pages: 941-946
    |
    Published online on: January 3, 2013
       https://doi.org/10.3892/mmr.2013.1260
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Abstract

Previous studies have demonstrated that calcium channel blockers have protective effects on damaged brains. In the present study, the protective effect of the calcium channel blocker nicardipine against rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells was investigated, focusing on mitogen‑activated protein kinases (MAPKs) and caspase (CASP)‑mediated apoptotic events. Nicardipine was found to decrease rotenone‑induced apoptosis through 4,6‑diamidino‑2‑phenylindole staining and the terminal deoxynucleotidyl transferase‑mediated dUTP nick end‑labeling assay. In addition, nicardipine was identified to inhibit rotenone‑induced elevation of intracellular Ca2+ concentration measured using the Fluo‑4 AM fluorescent dye. Rotenone increased phosphorylation of c‑Jun NH2‑terminal kinase (JNK) and p38 MAPK, whereas nicardipine blocked these increases. Nicardipine also prevented downregulation of B‑cell CLL/lymphoma 2 and upregulation of Bcl2‑associated X protein by rotenone. Furthermore, nicardipine abrogated cleavage of CASP9 and 3 and poly (ADP‑ribose) polymerase‑1 by rotenone and CASP3 enzyme activity in rotenone‑treated cells. These results indicate that nicardipine exerts a protective effect against rotenone‑induced apoptosis in SH‑SY5Y cells, inhibiting phosphorylation of JNK and p38 MAPK and activation of CASPs.
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Copy and paste a formatted citation
Spandidos Publications style
Park HJ and Kim HJ: Inhibitory effect of nicardipine on rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells. Mol Med Rep 7: 941-946, 2013.
APA
Park, H.J., & Kim, H. (2013). Inhibitory effect of nicardipine on rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells. Molecular Medicine Reports, 7, 941-946. https://doi.org/10.3892/mmr.2013.1260
MLA
Park, H. J., Kim, H."Inhibitory effect of nicardipine on rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells". Molecular Medicine Reports 7.3 (2013): 941-946.
Chicago
Park, H. J., Kim, H."Inhibitory effect of nicardipine on rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells". Molecular Medicine Reports 7, no. 3 (2013): 941-946. https://doi.org/10.3892/mmr.2013.1260
Copy and paste a formatted citation
x
Spandidos Publications style
Park HJ and Kim HJ: Inhibitory effect of nicardipine on rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells. Mol Med Rep 7: 941-946, 2013.
APA
Park, H.J., & Kim, H. (2013). Inhibitory effect of nicardipine on rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells. Molecular Medicine Reports, 7, 941-946. https://doi.org/10.3892/mmr.2013.1260
MLA
Park, H. J., Kim, H."Inhibitory effect of nicardipine on rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells". Molecular Medicine Reports 7.3 (2013): 941-946.
Chicago
Park, H. J., Kim, H."Inhibitory effect of nicardipine on rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells". Molecular Medicine Reports 7, no. 3 (2013): 941-946. https://doi.org/10.3892/mmr.2013.1260
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