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Article

The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase‑dependent and ‑independent pathways

  • Authors:
    • Giou‑Teng Yiang
    • Yen‑Hsieh Chen
    • Pei‑Lun Chou
    • Wei‑Jung Chang
    • Chyou‑Wei Wei
    • Yung‑Luen Yu
  • View Affiliations / Copyright

    Affiliations: Department of Emergency Medicine, Tzu Chi University, Hualien 970, Taiwan, R.O.C., Department of Life Science, Fu Jen Catholic University, New Taipei City 242, Taiwan, R.O.C., Department of Internal Medicine, Division of Allergy‑Immunology‑Rheumatology, Saint Mary's Hospital, Luodong 265, Taiwan, R.O.C., Graduate Institute of Cancer Biology  and Center for Molecular Medicine, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Nutrition, Hungkuang University, Taichung 433, Taiwan, R.O.C., Graduate Institute of Cancer Biology, China Medical University, 6 Hsueh‑Shih Road Taichung 404, Taiwan, R.O.C.
  • Pages: 826-830
    |
    Published online on: January 3, 2013
       https://doi.org/10.3892/mmr.2013.1261
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Abstract

Japanese encephalitis virus (JEV), a mosquito‑borne flavivirus, causes acute encephalitis and nervous damage. Previous studies have demonstrated that JEV induces apoptosis in infected cells. However, to date the mechanisms of JEV‑induced apoptosis are unclear. In order to identify the viral proteins associated with JEV‑induced apoptosis, pEGFP‑non‑structural protein 3 (NS3) 1‑619 (expressing the JEV NS3 intact protein, including the protease and helicase domains), pEGFP‑NS3 1‑180 (expressing the protease domain) and pEGFP‑NS3 163‑619 (expressing the helicase domain) were transfected into target cells to study cell death. Results demonstrate that the JEV NS3 intact protein and protease and helicase domains induce cell death. In addition, cell death was identified to be significantly higher in cells transfected with the NS3 protease domain compared with the intact protein and helicase domain. Caspase activation was also analyzed in the current study. NS3 intact protein and NS3 protease and helicase domains activated caspase‑9/‑3‑dependent and ‑independent pathways. However, caspase‑8 activity was not found to be significantly different in NS3‑transfected cells compared with control. In summary, the present study demonstrates that the NS3 helicase and protease domains of JEV activate caspase‑9/‑3‑dependent and ‑independent cascades and trigger cell death.
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Copy and paste a formatted citation
Spandidos Publications style
Yiang GT, Chen YH, Chou PL, Chang WJ, Wei CW and Yu YL: The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase‑dependent and ‑independent pathways. Mol Med Rep 7: 826-830, 2013.
APA
Yiang, G., Chen, Y., Chou, P., Chang, W., Wei, C., & Yu, Y. (2013). The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase‑dependent and ‑independent pathways. Molecular Medicine Reports, 7, 826-830. https://doi.org/10.3892/mmr.2013.1261
MLA
Yiang, G., Chen, Y., Chou, P., Chang, W., Wei, C., Yu, Y."The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase‑dependent and ‑independent pathways". Molecular Medicine Reports 7.3 (2013): 826-830.
Chicago
Yiang, G., Chen, Y., Chou, P., Chang, W., Wei, C., Yu, Y."The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase‑dependent and ‑independent pathways". Molecular Medicine Reports 7, no. 3 (2013): 826-830. https://doi.org/10.3892/mmr.2013.1261
Copy and paste a formatted citation
x
Spandidos Publications style
Yiang GT, Chen YH, Chou PL, Chang WJ, Wei CW and Yu YL: The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase‑dependent and ‑independent pathways. Mol Med Rep 7: 826-830, 2013.
APA
Yiang, G., Chen, Y., Chou, P., Chang, W., Wei, C., & Yu, Y. (2013). The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase‑dependent and ‑independent pathways. Molecular Medicine Reports, 7, 826-830. https://doi.org/10.3892/mmr.2013.1261
MLA
Yiang, G., Chen, Y., Chou, P., Chang, W., Wei, C., Yu, Y."The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase‑dependent and ‑independent pathways". Molecular Medicine Reports 7.3 (2013): 826-830.
Chicago
Yiang, G., Chen, Y., Chou, P., Chang, W., Wei, C., Yu, Y."The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase‑dependent and ‑independent pathways". Molecular Medicine Reports 7, no. 3 (2013): 826-830. https://doi.org/10.3892/mmr.2013.1261
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