Clinical evaluation of the levels of 12 cytokines in serum/plasma under various storage conditions using evidence biochip arrays

Guo,Guang‑Hong; Dong,Jin; Yuan,Xin‑Hong; Dong,Zhen‑Nan; Tian,Ya‑Ping

doi:10.3892/mmr.2013.1263

March 2013 Volume 7 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

March 2013 Volume 7 Issue 3

Full Size Image

Article

Clinical evaluation of the levels of 12 cytokines in serum/plasma under various storage conditions using evidence biochip arrays

Authors:
- Guang‑Hong Guo
- Jin Dong
- Xin‑Hong Yuan
- Zhen‑Nan Dong
- Ya‑Ping Tian
View Affiliations / Copyright

Affiliations: Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, P.R. China
Pages: 775-780
|
Published online on: January 4, 2013

https://doi.org/10.3892/mmr.2013.1263
Expand metrics +

Abstract

Cytokines are a group of peptides which form a sophisticated network to modulate multiple cellular events. Within such a network, and through complex feedback mechanisms, cytokine functions are largely interdependent and closely associated with a number of pathological processes. In the present study, the EVIDENCE 180 system was used to study the effects of storage temperature and repeated freeze/thaw cycles on the concentration of 12 cytokines in various sample types. Samples were collected from 9 healthy volunteers and stored by 3 methods: gel, glass and lithium heparin (LH) tubes. Immediately following collection, the concentration of each cytokine in the samples was measured. Cytokine concentrations of the 3 sample types that did not undergo repeated freeze/thaw cycles were compared with those subjected to 1‑10 freeze/thaw cycles. In addition, the dynamic changes of 6 sample types which were stored at 4˚C for 6 h to 6 days was analyzed. In addition, the within‑ and between‑run precision of 12 cytokines on the biochip array was evaluated. Interleukin (IL)‑8, vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) concentrations were lower in plasma compared with serum. Cytokine levels in serum and plasma were affected by several freeze/thaw cycles with IL‑1β, ‑4 and ‑10 increasing significantly following 1 freeze/thaw cycle and remaining at stable increased levels for the duration of the additional 9 cycles. In separated serum samples in gel and glass tubes stored at 4˚C for 6 days, no difference in concentration of the 12 cytokines was identified. In the other 4 sample types, IL‑8, VEGF, tumor necrosis factor α and EGF levels were altered when stored at 4˚C. Results indicate that the EVIDENCE 180 system is stable and plasma was observed as the best sample type to determine concentration of the 12 cytokines using this biochip array. Repeated freeze/thaw cycles and storage at 4˚C was identified to affect the concentration of the 12 cytokines. The current study demonstrates that repeated freeze/thaw cycles of samples must be avoid. In addition, results indicate that plasma or serum must be separated immediately following centrifugation and sample concentration should be measured as soon as possible.

View Figures

View References

1	Berrahmoune H, Lamont JV, Herbeth B, FitzGerald PS and Visvikis-Siest S: Biological determinants of and reference values for plasma interleukin-8, monocyte chemoattractant protein-1, epidermal growth factor and vascular endothelial growth factor: Results from the STANISLAS cohort. Clin Chem. 52:504–510. 2006. View Article : Google Scholar
2	Beamer GL, Flaherty DK, Assogba BD, Stromberg P, Gonzalez-Juarrero M, de Waal Malefyt R, Vesosky B and Turner J: Interleukin-10 promotes Mycobacterium tuberculosis disease progression in CBA/J mice. J Immunol. 181:5545–5550. 2008.PubMed/NCBI
3	Londono D, Carvajal J, Arguelles-Grande C, Marques A and Cadavid D: Interleukin 10 protects the brain microcirculation from spirochetal injury. J Neuropathol Exp Neurol. 67:976–983. 2008. View Article : Google Scholar : PubMed/NCBI
4	Rautert R, Schinkothe T, Franklin J, Weihrauch M, Boll B, Pogge E, Bredenfeld H, Engert A, Diehl V and Re D: Elevated pretreatment interleukin-10 serum level is an International Prognostic Score (IPS)-independent risk factor for early treatment failure in advanced stage Hodgkin lymphoma. Leuk Lymphoma. 49:2091–2098. 2008. View Article : Google Scholar
5	Karadag F, Karul AB, Cildag O, Yilmaz M and Ozcan H: Biomarkers of systemic inflammation in stable and exacerbation phases of COPD. Lung. 186:403–409. 2008. View Article : Google Scholar : PubMed/NCBI
6	Kosmala W, Derzhko R, Przewlocka-Kosmala M, Orda A and Mazurek W: Plasma levels of TNF-alpha, IL-6 and IL-10 and their relationship with left ventricular diastolic function in patients with stable angina pectoris and preserved left ventricular systolic performance. Coron Artery Dis. 19:375–382. 2008. View Article : Google Scholar
7	Jha HC, Srivastava P, Sarkar R, Prasad J and Mittal A: Chlamydia pneumoniae IgA and elevated level of IL-6 may synergize to accelerate coronary artery disease. J Cardiol. 52:140–145. 2008. View Article : Google Scholar
8	Moreno I, Mir A, Vicente R, Pajares A, Ramos F, Vicente JL and Barbera M: Analysis of interleukin-6 and interleukin-8 in lung transplantation: correlation with nitric oxide administration. Transplant Proc. 40:3082–3084. 2008. View Article : Google Scholar : PubMed/NCBI
9	Nee L, O'Connell S, Nolan S, Ryan MP and McMorrow T: Nitric oxide involvement in TNF-alpha and IL-1 beta-mediated changes in human mesangial cell MMP-9 and TIMP-1. Nephron Exp Nephrol. 110:e59–e66. 2008. View Article : Google Scholar : PubMed/NCBI
10	Cheung GT, Siow YL and OK: Homocysteine stimulates monocyte chemoattractant protein-1 expression in mesangial cells via NF-kappaB activation. Can J Physiol Pharmacol. 86:88–96. 2008. View Article : Google Scholar : PubMed/NCBI
11	Yasui T, Uemura H, Yamada M, Matsuzaki T, Tsuchiya N, Noguchi M, Yuzurihara M, Kase Y and Irahara M: Associations of interleukin-6 with interleukin-1beta, interleukin-8 and macrophage inflammatory protein-1beta in midlife women. Cytokine. 41:302–306. 2008. View Article : Google Scholar : PubMed/NCBI
12	Lee KY, Lee KS, Park SJ, Kim SR, Min KH, Choe YH and Lee YC: Clinical significance of plasma and serum vascular endothelial growth factor in asthma. J Asthma. 45:735–739. 2008. View Article : Google Scholar : PubMed/NCBI
13	Schoonenboom NS, Mulder C, Vanderstichele H, Van Elk EJ, Kok A, Van Kamp GJ, Scheltens P and Blankenstein MA: Effects of processing and storage conditions on amyloid beta (1–42) and tau concentrations in cerebrospinal fluid: implications for use in clinical practice. Clin Chem. 51:189–195. 2005.
14	Fitzgerald SP, Lamont JV, McConnell RI and Benchikhel O: Development of a high-throughput automated analyzer using biochip array technology. Clin Chem. 51:1165–1176. 2005. View Article : Google Scholar : PubMed/NCBI
15	Matei I and Matei L: Cytokine patterns and pathogenicity in autoimmune diseases. Rom J Intern Med. 40:27–41. 2002.PubMed/NCBI
16	Moser B and Willimann K: Chemokines: role in inflammation and immune surveillance. Ann Rheum Dis. 63(Suppl 2): ii84–ii89. 2004. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Clinical evaluation of the levels of 12 cytokines in serum/plasma under various storage conditions using evidence biochip arrays

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Related Articles