Effect of chronic renal failure medium on the ubiquitin‑proteasome pathway of arterial muscle cells

Zhang,Yao‑Quan; Feng,Bing; Yuan,Fa‑Huan

doi:10.3892/mmr.2013.1269

March 2013 Volume 7 Issue 3

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March 2013 Volume 7 Issue 3

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Article

Effect of chronic renal failure medium on the ubiquitin‑proteasome pathway of arterial muscle cells

Authors:
- Yao‑Quan Zhang
- Bing Feng
- Fa‑Huan Yuan
View Affiliations / Copyright

Affiliations: Department of Nephrology, the Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin 214400, P.R. China, Department of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China
Pages: 1021-1025
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Published online on: January 11, 2013

https://doi.org/10.3892/mmr.2013.1269
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Abstract

The ubiquitin‑proteasome pathway (UPP) is involved in the occurrence and development of atherosclerosis through inhibitor of κB (IκB) degradation which activates nuclear factor-κB (NF‑κB). However, the correlation between UPP and vascular complications of uramia remains unknown. The aim of the present study was to determine whether the UPP is activated in aortic smooth muscle cells (ASMCs) when cultured with uremic serum and to examine the role of the UPP on the dysfunction of ASMCs in uremia. ASMCs were cultured with pooled normal sera or chronic renal failure sera. The mRNA expression levels for ubiquitin (Ub) and Ub-activating enzyme (E1) were analyzed using reverse transcription PCR and levels of the ubiquitinated proteins E1 and IκBα were measured using western blot analysis. The enzymatic activities of three 20S proteasomes were examined using specific fluorogenic peptide substrates. Compared with normal serum, chronic renal serum increased E1 mRNA and protein expression of rabbit ASMCs (both P<0.01). In addition, the mRNA expression of Ub also increased and the expression of IκBα was observed to decrease significantly (both P<0.01). Ubiquitinated proteins in the normal and chronic renal failure groups were not found to be significantly different, but the activity of proteasomes increased significantly (P<0.01). Chronic renal failure medium induced the activation of the UPP in ASMCs.

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1	Locatelli F, Pozzoni P, Tentori F and del Vecchio L: Epidemiology of cardiovascular risk in patients with chronic kidney disease. Nephrol Dial Transplant. 18:2–9. 2003. View Article : Google Scholar : PubMed/NCBI
2	Hou FF, Ma ZG, Mei CL, et al: Epidemiology of cardiovascular risk in Chinese chronic kidney disease patients. Zhonghua Yi Xue Za Zhi. 85:753–759. 2005.(In Chinese).
3	Mitch WE, Du J, Bailey JL and Price SR: Mechanisms causing muscle proteolysis in uremia: the influence of insulin and cytokines. Miner Electrolyte Metab. 25:216–219. 1999. View Article : Google Scholar : PubMed/NCBI
4	Price SR, Du JD, Bailey JL and Mitch WE: Molecular mechanisms regulating protein turnover in muscle. Am J Kidney Dis. 37(Suppl 2): 112–114. 2001. View Article : Google Scholar : PubMed/NCBI
5	Mitch WE: Robert H Herman Memorial Award in Clinical Nutrition Lecture, 1997. Mechanisms causing loss of lean body mass in kidney disease. Am J Clin Nutr. 67:359–366. 1998.PubMed/NCBI
6	Fukai T: Targeting proteasome worsens atherosclerosis. Circ Res. 101:859–861. 2007. View Article : Google Scholar : PubMed/NCBI
7	Herrmann J, Soares SM, Lerman LO and Lerman A: Potential role of the ubiquitin-proteasome system in atherosclerosis: aspects of a protein quality disease. J Am Coll Cardiol. 51:2003–2010. 2008. View Article : Google Scholar : PubMed/NCBI
8	Feng B, Zhang Y, Mu J, et al: Preventive effect of a proteasome inhibitor on the formation of accelerated atherosclerosis in rabbits with uremia. J Cardiovasc Pharmacol. 55:129–138. 2010. View Article : Google Scholar : PubMed/NCBI
9	Tao R, Lu L, Zhang R, Hu J, Ni J and Shen W: Triptolide inhibits rat vascular smooth muscle cell proliferation and cell cycle progression via attenuation of ERK1/2 and Rb phosphorylation. Exp Mol Pathol. 90:137–142. 2011. View Article : Google Scholar : PubMed/NCBI
10	Little PJ, Ivey ME and Osman N: Endothelin-1 actions on vascular smooth muscle cell functions as a target for the prevention of atherosclerosis. Curr Vasc Pharmacol. 6:195–203. 2008. View Article : Google Scholar : PubMed/NCBI
11	Chung SW, Park JW, Lee SA, Eo SK and Kim K: Thrombin promotes proinflammatory phenotype in human vascular smooth muscle cell. Biochem Biophys Res Commun. 396:748–754. 2010. View Article : Google Scholar : PubMed/NCBI
12	Newby AC: Matrix metalloproteinases regulate migration, proliferation and death of vascular smooth muscle cells by degrading matrix and non-matrix substrates. Cardiovasc Res. 69:614–624. 2006. View Article : Google Scholar
13	Murata T and Shimotohno K: Ubiquitination and proteasome-dependent degradation of human eukaryotic translation initiation factor 4E. J Biol Chem. 281:20788–20800. 2006. View Article : Google Scholar : PubMed/NCBI
14	Murray AW: Recycling the cell cycle: cyclins revisited. Cell. 116:221–234. 2004. View Article : Google Scholar : PubMed/NCBI
15	Ciechanover A and Iwai K: The ubiquitin proteolytic system: from an idea to the patient bed. Proc Am Thorac Soc. 3:21–31. 2006. View Article : Google Scholar : PubMed/NCBI
16	Gomes AV, Young GW, Wang Y, et al: Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues. Mol Cell Proteomics. 8:302–315. 2009. View Article : Google Scholar : PubMed/NCBI
17	Drews O, Wildgruber R and Zong C: Mammalian proteasome subpopulations with distinct molecular compositions and proteolytic activities. Mol Cell Proteomics. 6:2021–2031. 2007. View Article : Google Scholar : PubMed/NCBI
18	Groll M, Ditzel L, Löwe J, et al: Structure of the 20S proteasome from yeast at 2.4 A resolution. Nature. 386:463–471. 1997. View Article : Google Scholar : PubMed/NCBI
19	Carbó C, Arderiu G, Escolar G, et al: Differential expression of proteins from cultured endothelial cells exposed to uremic versus normal serum. Am J Kidney Dis. 51:603–612. 2008.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of chronic renal failure medium on the ubiquitin‑proteasome pathway of arterial muscle cells

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