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Article

ADAM15 is involved in MICB shedding and mediates the effects of gemcitabine on MICB shedding in PANC-1 pancreatic cancer cells

  • Authors:
    • Xiaohui Duan
    • Xianhai Mao
    • Weijia Sun
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha 410005, P.R. China, Department of Gerneral Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China
  • Pages: 991-997
    |
    Published online on: January 11, 2013
       https://doi.org/10.3892/mmr.2013.1272
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Abstract

The aim of this study was to investigate the role of ADAM15 in MHC class I polypeptide-related sequence B (MICB) protein ectodomain shedding and observe whether or not gemcitabine affects MICB shedding from PANC-1 cells. In this study, immunohistochemistry of MICB and ADAM15 were performed on tumor samples obtained from 93 patients with pancreatic ductal adenocarcinoma (PDAC). The expression of MICB and ADAM15 in the PDAC tissues was significantly higher compared with that in the normal tissues of the pancreas. Statistical analysis showed a significant correlation between the expression of MICB and certain classic clinicopathological characteristics (i.e., histological grade and TNM stage). ADAM15 expression was found to correlate with lymph node metastasis and TNM stage. The Spearman's rank test suggested that the expression of MICB was inversely correlated with that of ADAM15 in PDAC tissues. Knockdown of ADAM15 in PANC-1 cells clearly upregulated MICB expression on the cellular surface and downregulated soluble MICB (sMICB) levels in the culture supernatants. A non-toxic dose of 0.5 µmol/l gemcitabine suppresses ADAM15 expression leading, at the same time, to an increase in MICB expression and a decrease in sMICB production in PANC-1 cells. The mRNA levels of MICB did not change following PANC-1 exposure to gemcitabine. Further study suggests that the suppressive effect of gemcitabine on MICB shedding in PANC-1 cells is mediated by ADAM15 downregulation. In conclusion, the results of the present study support the hypothesis that ADAM15 is involved in MICB shedding of PANC-1 cells and that gemcitabine inhibits MICB ectodomain shedding through the suppression of ADAM15.
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Copy and paste a formatted citation
Spandidos Publications style
Duan X, Mao X and Sun W: ADAM15 is involved in MICB shedding and mediates the effects of gemcitabine on MICB shedding in PANC-1 pancreatic cancer cells. Mol Med Rep 7: 991-997, 2013.
APA
Duan, X., Mao, X., & Sun, W. (2013). ADAM15 is involved in MICB shedding and mediates the effects of gemcitabine on MICB shedding in PANC-1 pancreatic cancer cells. Molecular Medicine Reports, 7, 991-997. https://doi.org/10.3892/mmr.2013.1272
MLA
Duan, X., Mao, X., Sun, W."ADAM15 is involved in MICB shedding and mediates the effects of gemcitabine on MICB shedding in PANC-1 pancreatic cancer cells". Molecular Medicine Reports 7.3 (2013): 991-997.
Chicago
Duan, X., Mao, X., Sun, W."ADAM15 is involved in MICB shedding and mediates the effects of gemcitabine on MICB shedding in PANC-1 pancreatic cancer cells". Molecular Medicine Reports 7, no. 3 (2013): 991-997. https://doi.org/10.3892/mmr.2013.1272
Copy and paste a formatted citation
x
Spandidos Publications style
Duan X, Mao X and Sun W: ADAM15 is involved in MICB shedding and mediates the effects of gemcitabine on MICB shedding in PANC-1 pancreatic cancer cells. Mol Med Rep 7: 991-997, 2013.
APA
Duan, X., Mao, X., & Sun, W. (2013). ADAM15 is involved in MICB shedding and mediates the effects of gemcitabine on MICB shedding in PANC-1 pancreatic cancer cells. Molecular Medicine Reports, 7, 991-997. https://doi.org/10.3892/mmr.2013.1272
MLA
Duan, X., Mao, X., Sun, W."ADAM15 is involved in MICB shedding and mediates the effects of gemcitabine on MICB shedding in PANC-1 pancreatic cancer cells". Molecular Medicine Reports 7.3 (2013): 991-997.
Chicago
Duan, X., Mao, X., Sun, W."ADAM15 is involved in MICB shedding and mediates the effects of gemcitabine on MICB shedding in PANC-1 pancreatic cancer cells". Molecular Medicine Reports 7, no. 3 (2013): 991-997. https://doi.org/10.3892/mmr.2013.1272
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