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Article

The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis

  • Authors:
    • Wen Gao
    • Wei Xu
    • Hongli Li
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Shanghai First People's Hospital, College of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China, Department of Interventional Radiology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
  • Pages: 862-868
    |
    Published online on: January 21, 2013
       https://doi.org/10.3892/mmr.2013.1282
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Abstract

Peroxisome proliferator-activated receptor α (PPAR-α) has been detected in the liver, kidney, heart and skeletal muscle. The expression and mechanism of PPAR-α in atherosclerosis remains unclear. The present study was undertaken in order to examine the expression and role of PPAR-α in programmed atherosclerosis induced by a high‑fat diet and balloon-injury in rabbits. Rabbits were randomly divided into 3 groups: control, high-fat diet and high-fat diet + balloon‑injury groups. The high-fat diet and high-fat diet + balloon-injury groups were further divided into 6-, 8- and 10-week groups. Real-time quantitative PCR analysis was used to detect PPAR-α mRNA and immunohistochemistry (IHC) and western blot analysis were used to examine PPAR-α protein expression. Tumor necrosis factor (TNF)-α, interleukin (IL)-10 and P-selectin levels in the rabbits were measured by enzyme-linked immunosorbent assay (ELISA). In the high-fat or high-fat diet + balloon-injury groups, the vascular thickness was markedly higher than in the control group (P<0.01). PPAR-α protein and mRNA were significantly increased in the high-fat diet group as compared with the control group (P<0.01). Furthermore, there were marked changes from 6 to 10 weeks in the high-fat diet group (P<0.01). Compared with the control group, PPAR-α protein and mRNA were increased in the high-fat diet + balloon-injury group (P<0.01). There were significant differences of PPAR-α protein and mRNA at various time points in the high-fat diet + balloon‑injury group, as shown by real-time quantitative PCR and IHC (P<0.01). As shown by western blotting, there were no differences between the high-fat diet + balloon-injury 8- and 10-week groups (P>0.05). In those arteries that were occluded by ≥60%, PPAR-α expression was lower than that in the arteries which were occluded <60% in the high‑fat diet + balloon-injury 10-week group. In the high-fat diet and high-fat diet + balloon-injury groups, the levels of IL-10, TNF-α and P-selectin were upregulated compared with the control group. However, from weeks 8 to 10, TNF-α and P-selectin were decreased and IL-10 was still increased in the high-fat diet + balloon-injury group. The results of this study demonstrate that PPAR-α has preventive effects on atherosclerosis, which may be related to the regulation of inflammation.
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Copy and paste a formatted citation
Spandidos Publications style
Gao W, Xu W and Li H: The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis. Mol Med Rep 7: 862-868, 2013.
APA
Gao, W., Xu, W., & Li, H. (2013). The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis. Molecular Medicine Reports, 7, 862-868. https://doi.org/10.3892/mmr.2013.1282
MLA
Gao, W., Xu, W., Li, H."The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis". Molecular Medicine Reports 7.3 (2013): 862-868.
Chicago
Gao, W., Xu, W., Li, H."The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis". Molecular Medicine Reports 7, no. 3 (2013): 862-868. https://doi.org/10.3892/mmr.2013.1282
Copy and paste a formatted citation
x
Spandidos Publications style
Gao W, Xu W and Li H: The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis. Mol Med Rep 7: 862-868, 2013.
APA
Gao, W., Xu, W., & Li, H. (2013). The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis. Molecular Medicine Reports, 7, 862-868. https://doi.org/10.3892/mmr.2013.1282
MLA
Gao, W., Xu, W., Li, H."The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis". Molecular Medicine Reports 7.3 (2013): 862-868.
Chicago
Gao, W., Xu, W., Li, H."The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis". Molecular Medicine Reports 7, no. 3 (2013): 862-868. https://doi.org/10.3892/mmr.2013.1282
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