Achyranthes bidentata polysaccharides induce chondrocyte proliferation via the promotion of the G1/S cell cycle transition

Yu,Fangrong; Li,Xihai; Cai,Liangliang; Li,Huiting; Chen,Jiashou; Wong,Xiaping; Xu,Huifeng; Zheng,Chunsong; Liu,Xianxiang; Ye,Hongzhi

doi:10.3892/mmr.2013.1286

March 2013 Volume 7 Issue 3

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March 2013 Volume 7 Issue 3

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Article

Achyranthes bidentata polysaccharides induce chondrocyte proliferation via the promotion of the G1/S cell cycle transition

Authors:
- Fangrong Yu
- Xihai Li
- Liangliang Cai
- Huiting Li
- Jiashou Chen
- Xiaping Wong
- Huifeng Xu
- Chunsong Zheng
- Xianxiang Liu
- Hongzhi Ye
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Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
Pages: 935-940
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Published online on: January 22, 2013

https://doi.org/10.3892/mmr.2013.1286
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Abstract

Achyranthes bidentata polysaccharides (ABPS) are the major bioactive constituents of Radix Achyranthes bidentata (AB), which has been widely used in traditional Chinese medicine for the treatment of osteoarthritis. However, the molecular mechanisms behind the therapeutic effect of ABPS remain unclear. In the present study, chondrocytes were isolated from Sprague-Dawley rats. The effects of ABPS on the G1/S cell cycle transition in primary chondrocytes were investigated. The chondrocytes treated with and without ABPS were analyzed and it was observed that ABPS treatment was able to enhance chondrocyte proliferation in a dose- and time-dependent manner and promote the progression of chondrocyte cell cycle proliferation via the promotion of the G1 to S phase transition. Furthermore, using RT-PCR and western blot analysis, ABPS were observed as significantly upregulating the expression of cyclin D1 and the cyclin-dependent kinases (CDKs) CDK4 and CDK6. These results suggest that ABPS are able to promote chondrocyte proliferation via the promotion of the G1/S cell cycle transition.

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1	Iliopoulos D, Gkretsi V and Tsezou A: Proteomics of osteoarthritic chondrocytes and cartilage. Expert Rev Proteomics. 7:749–760. 2010. View Article : Google Scholar : PubMed/NCBI
2	Lotz MK and Caramés B: Autophagy and cartilage homeostasis mechanisms in joint health, aging and OA. Nat Rev Rheumatol. 7:579–587. 2011.PubMed/NCBI
3	Clouet J, Vinatier C, Merceron C, Pot-vaucel M, Maugars Y, Weiss P, Grimandi G and Guicheux J: From osteoarthritis treatments to future regenerative therapies for cartilage. Drug Discov Today. 14:913–925. 2009. View Article : Google Scholar : PubMed/NCBI
4	Schroeppel JP, Crist JD, Anderson HC and Wang J: Molecular regulation of articular chondrocyte function and its significance in osteoarthritis. Histol Histopathol. 26:377–394. 2011.PubMed/NCBI
5	Zhang M, Xie R, Hou W, Wang B, Shen R, Wang X, Wang Q, Zhu T, Jonason JH and Chen D: PTHrP prevents chondrocyte premature hypertrophy by inducing cyclin-D1-dependent Runx2 and Runx3 phosphorylation, ubiquitylation and proteasomal degradation. J Cell Sci. 122:1382–1389. 2009. View Article : Google Scholar : PubMed/NCBI
6	Golias CH, Charalabopoulos A and Charalabopoulos K: Cell proliferation and cell cycle control: a mini review. Int J Clin Pract. 58:1134–1141. 2004. View Article : Google Scholar : PubMed/NCBI
7	Blagosklonny MV and Pardee AB: The restriction point of the cell cycle. Cell Cycle. 1:103–110. 2002. View Article : Google Scholar : PubMed/NCBI
8	Li X, Ye H, Yu F, et al: Millimeter wave treatment promotes chondrocytes proliferation via G₁/S cell cycle transition. Int J Mol Med. 29:823–831. 2012.PubMed/NCBI
9	Chen Q, Liu Z and He J: Achyranthes bidentata polysaccharide enhances immune response in weaned piglets. Immunopharmacol Immunotoxicol. 31:253–260. 2009. View Article : Google Scholar
10	Xue JP and Shi JY: Primary study on suspension cell culture and polysaccharides content of Achyranthes bidentata. Zhongguo Zhong Yao Za Zhi. 33:2467–2469. 2008.(In Chinese).
11	Chen XM, Xu YJ and Tian GY: Physical-chemical properties and structure elucidation of abPS isolated from the root of Achyranthes bidentata. Yao Xue Xue Bao. 40:32–35. 2005.(In Chinese).
12	Peng ZG, Chen HS, Guo ZM, Dong B, Tian GY and Wang GQ: Anti-HIV activites of Achyranthes bidentata polysaccharide sulfate in vitro and in vivo. Yao Xue Xue Bao. 43:702–706. 2008.(In Chinese).
13	Zhu X, Pan Y, Zheng L, Cui L and Cao Y: Polysaccharides from the Chinese medicine herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice. Malar J. 11:492012.
14	Zou Y, Meng J, Chen W, Liu J, Li X, Li W, Lu C and Shan F: Modulation of phenotypic and functional maturation of murine dendritic cells (DCs) by purified Achyranthes bidentata polysaccharide (ABP). Int Immunopharmacol. 11:1103–1108. 2011. View Article : Google Scholar : PubMed/NCBI
15	Lin J, Zhang Z and Shan Y: Effect of Achyranthes bidentata polysaccharides on the expression of BCL-2 and bax in hepatic tissues after exhaustive exercise in rats. Afr J Tradit Complement Altern Med. 7:307–314. 2010.
16	Boddohi S and Kipper MJ: Engineering nanoassemblies of polysaccharides. Adv Mater. 22:2998–3016. 2010. View Article : Google Scholar : PubMed/NCBI
17	Harris JD, Siston RA, Pan X and Flanigan DC: Autologous chondrocyte implantation: a systematic review. J Bone Joint Surg Am. 92:2220–2233. 2010. View Article : Google Scholar : PubMed/NCBI
18	Chan BY, Fuller ES, Russell AK, et al: Increased chondrocyte sclerostin may protect against cartilage degradation in osteoarthritis. Osteoarthritis Cartilage. 19:874–885. 2011. View Article : Google Scholar : PubMed/NCBI
19	Onumah OE, Jules GE, Zhao Y, Zhou L, Yang H and Guo Z: Overexpression of catalase delays G₀/G₁-to S-phase transition during cell cycle progression in mouse aortic endothelial cells. Free Radic Biol Med. 46:1658–1667. 2009.PubMed/NCBI
20	Pietras EM, Warr MR and Passegué E: Cell cycle regulation in hematopoietic stem cells. J Cell Biol. 195:709–720. 2011. View Article : Google Scholar : PubMed/NCBI
21	Ogasawara T, Mori Y, Abe M, et al: Role of cyclin-dependent kinase (Cdk)6 in osteoblast, osteoclast, and chondrocyte differentiation and its potential as a target of bone regenerative medicine. Oral Science International. 8:2–6. 2011. View Article : Google Scholar
22	Echalier A, Endicott JA and Noble ME: Recent developments in cyclin-dependent kinase biochemical and structural studies. Biochim Biophys Acta. 1804:511–519. 2010. View Article : Google Scholar : PubMed/NCBI
23	Inagaki S and Umeda M: Cell-cycle control and plant development. Int Rev Cell Mol Biol. 291:227–261. 2011. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Achyranthes bidentata polysaccharides induce chondrocyte proliferation via the promotion of the G1/S cell cycle transition

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