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Article

Human endogenous retrovirus-H insertion screening

  • Authors:
    • Mehrab Guliyev
    • Sibel Yilmaz
    • Kaniye Sahin
    • Sevgi Marakli
    • Nermin Gozukirmizi
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, Istanbul 34134, Turkey
  • Pages: 1305-1309
    |
    Published online on: January 28, 2013
       https://doi.org/10.3892/mmr.2013.1295
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Abstract

Endogenous retroviruses (ERVs) and ERV-like sequences comprise 8% of the human genome. We aimed to analyze genome integration polymorphisms of human endogenous retrovirus (HERV)-H by the inter-retrotransposon amplified polymorphism (IRAP) technique using the sequences of LTR7A (450 bp), LTR7B (445 bp) and LTR7C (471 bp). Blood samples from 20 individuals (10 females and 10 males) of diverse ethnic origins were used for the determination of integration variations at the genomic level. Isolated genomic DNA was screened using 3 pairs of primers corresponding to LTR regions of the HERV-H gene. We observed insertion polymorphism patterns between 0-87% in all subjects. The findings obtained contribute to our understanding of the effects of HERV-H on variations within the human genome.
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Copy and paste a formatted citation
Spandidos Publications style
Guliyev M, Yilmaz S, Sahin K, Marakli S and Gozukirmizi N: Human endogenous retrovirus-H insertion screening. Mol Med Rep 7: 1305-1309, 2013.
APA
Guliyev, M., Yilmaz, S., Sahin, K., Marakli, S., & Gozukirmizi, N. (2013). Human endogenous retrovirus-H insertion screening. Molecular Medicine Reports, 7, 1305-1309. https://doi.org/10.3892/mmr.2013.1295
MLA
Guliyev, M., Yilmaz, S., Sahin, K., Marakli, S., Gozukirmizi, N."Human endogenous retrovirus-H insertion screening". Molecular Medicine Reports 7.4 (2013): 1305-1309.
Chicago
Guliyev, M., Yilmaz, S., Sahin, K., Marakli, S., Gozukirmizi, N."Human endogenous retrovirus-H insertion screening". Molecular Medicine Reports 7, no. 4 (2013): 1305-1309. https://doi.org/10.3892/mmr.2013.1295
Copy and paste a formatted citation
x
Spandidos Publications style
Guliyev M, Yilmaz S, Sahin K, Marakli S and Gozukirmizi N: Human endogenous retrovirus-H insertion screening. Mol Med Rep 7: 1305-1309, 2013.
APA
Guliyev, M., Yilmaz, S., Sahin, K., Marakli, S., & Gozukirmizi, N. (2013). Human endogenous retrovirus-H insertion screening. Molecular Medicine Reports, 7, 1305-1309. https://doi.org/10.3892/mmr.2013.1295
MLA
Guliyev, M., Yilmaz, S., Sahin, K., Marakli, S., Gozukirmizi, N."Human endogenous retrovirus-H insertion screening". Molecular Medicine Reports 7.4 (2013): 1305-1309.
Chicago
Guliyev, M., Yilmaz, S., Sahin, K., Marakli, S., Gozukirmizi, N."Human endogenous retrovirus-H insertion screening". Molecular Medicine Reports 7, no. 4 (2013): 1305-1309. https://doi.org/10.3892/mmr.2013.1295
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